Leptin enhances cell migration in human chondrosarcoma cells through OBRl leptin receptor

Shu Ning Yang, Hsien Te Chen, Hsi Kai Tsou, Chun Yin Huang, Wei Hung Yang, Chen Ming Su, Yi Chin Fong, Wen Pei Tseng, Chih Hsin Tang

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36 Citations (Scopus)

Abstract

Leptin, an adipocyte-derived cytokine that is closely associated with obesity, has recently been shown to be involved in carcinogenesis and cancer progression. Integrins are the major adhesive molecules in mammalian cells and have been associated with metastasis of cancer cells. In this study, we found that leptin increased the migration and the expression of αvβ3 integrin in human chondrosarcoma cells. We also found that human chondrosarcoma tissues and chondrosarcoma cell lines had significant expression of the long form (OBRl) leptin receptor, which was higher than that in normal cartilage and human primary chondrocyte. Leptin-mediated migration and integrin upregulation were attenuated by OBRl receptor antisense oligonucleotide. Activations of insulin receptor substrate (IRS)-1, phosphatidylinositol 3-kinase (PI3K), Akt and nuclear factor-κB (NF-κB) pathways after leptin treatment were demonstrated, and leptin-induced expression of integrin and migration activity was inhibited by the specific inhibitor, small-interfering RNA and mutant of IRS-1, PI3K, Akt and NF-κB cascades. Taken together, our results indicated that leptin enhances the migration of chondrosarcoma cells by increasing αvβ3 integrin expression through the OBR1/IRS-1/PI3K/Akt/ NF-κB signal transduction pathway.

Original languageEnglish
Pages (from-to)566-574
Number of pages9
JournalCarcinogenesis
Volume30
Issue number4
DOIs
Publication statusPublished - 2009 Apr 17

All Science Journal Classification (ASJC) codes

  • Cancer Research

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    Yang, S. N., Chen, H. T., Tsou, H. K., Huang, C. Y., Yang, W. H., Su, C. M., Fong, Y. C., Tseng, W. P., & Tang, C. H. (2009). Leptin enhances cell migration in human chondrosarcoma cells through OBRl leptin receptor. Carcinogenesis, 30(4), 566-574. https://doi.org/10.1093/carcin/bgp023