Laser-induced fluorescence technique for DNA and proteins separated by capillary electrophoresis

Yang Wei Lin, Tai Chia Chiu, Huan Tsung Chang

Research output: Contribution to journalReview article

55 Citations (Scopus)

Abstract

Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength>500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE-LIF using He-Ne lasers. These systems are benefited from low background, low costs, easy maintenance, and compactness. Derivatizations of DNA and proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, and high efficiency, the applications of CE-LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, and single cell analysis have been presented.

Original languageEnglish
Pages (from-to)37-48
Number of pages12
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume793
Issue number1
DOIs
Publication statusPublished - 2003 Aug 5

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Lasers
Fluorescence
DNA
Proteins
Coloring Agents
Single-Cell Analysis
Trace analysis
Wavelength
Biopolymers
Polymerase chain reaction
Reaction products
DNA Sequence Analysis
Maintenance
Costs and Cost Analysis
Polymerase Chain Reaction
Costs

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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abstract = "Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength>500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE-LIF using He-Ne lasers. These systems are benefited from low background, low costs, easy maintenance, and compactness. Derivatizations of DNA and proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, and high efficiency, the applications of CE-LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, and single cell analysis have been presented.",
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Laser-induced fluorescence technique for DNA and proteins separated by capillary electrophoresis. / Lin, Yang Wei; Chiu, Tai Chia; Chang, Huan Tsung.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 793, No. 1, 05.08.2003, p. 37-48.

Research output: Contribution to journalReview article

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