Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe

Thommey P. Thomas, Jing Yong Ye, Yu Chung Chang, Alina Kotlyar, Zhengyi Cao, Istvan J. Majoros, Theodore B. Norris, James R. Baker

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Fluorescence quantification in tissues using conventional techniques can be difficult due to the absorption and scattering of light in tissues. Our previous studies have shown that a single-mode optical fiber (SMF)-based, two-photon optical fiber fluorescence (TPOFF) probe could be effective as a minimally invasive, real-time technique for quantifying fluorescence in solid tumors. We report improved results with this technique using a solid, double-clad optical fiber (DCF). The DCF can maintain a high excitation rate by propagating ultrashort laser pulses down an inner single-mode core, while demonstrating improved collection efficiency by using a high-numerical aperture multimode outer core confined with a second clad. We have compared the TPOFF detection efficiency of the DCF versus the SMF with standard solutions of the generation 5 poly(amidoamine) dendrimer (G5) nanoparticles G5-6TAMRA (G5-6T) and G5-6TAMRA-folic acid (G5-6T-FA). The DCF probe showed three- to five-fold increases in the detection efficiency of these conjugates, in comparison to the SMF. We also demonstrate the applicability of the DCF to quantify the targeted uptake of G5-6T-FA in mouse tumors expressing the FA receptor. These results indicate that the TPOFF technique using the DCF probe is an appropriate tool to quantify low nanomolar concentrations of targeted fluorescent probes from deep tissue.

Original languageEnglish
Article number014024
JournalJournal of Biomedical Optics
Volume13
Issue number1
DOIs
Publication statusPublished - 2008 Dec 1

Fingerprint

Dendrimers
dendrimers
Optical fibers
Tumors
tumors
optical fibers
Cells
Nanoparticles
nanoparticles
probes
Fluorescence
Single mode fibers
fluorescence
folic acid
Photons
Tissue
Folic Acid
photons
Acids
numerical aperture

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Thomas, Thommey P. ; Ye, Jing Yong ; Chang, Yu Chung ; Kotlyar, Alina ; Cao, Zhengyi ; Majoros, Istvan J. ; Norris, Theodore B. ; Baker, James R. / Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. In: Journal of Biomedical Optics. 2008 ; Vol. 13, No. 1.
@article{bc866e5ce9e7420da7203c83f8b86d10,
title = "Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe",
abstract = "Fluorescence quantification in tissues using conventional techniques can be difficult due to the absorption and scattering of light in tissues. Our previous studies have shown that a single-mode optical fiber (SMF)-based, two-photon optical fiber fluorescence (TPOFF) probe could be effective as a minimally invasive, real-time technique for quantifying fluorescence in solid tumors. We report improved results with this technique using a solid, double-clad optical fiber (DCF). The DCF can maintain a high excitation rate by propagating ultrashort laser pulses down an inner single-mode core, while demonstrating improved collection efficiency by using a high-numerical aperture multimode outer core confined with a second clad. We have compared the TPOFF detection efficiency of the DCF versus the SMF with standard solutions of the generation 5 poly(amidoamine) dendrimer (G5) nanoparticles G5-6TAMRA (G5-6T) and G5-6TAMRA-folic acid (G5-6T-FA). The DCF probe showed three- to five-fold increases in the detection efficiency of these conjugates, in comparison to the SMF. We also demonstrate the applicability of the DCF to quantify the targeted uptake of G5-6T-FA in mouse tumors expressing the FA receptor. These results indicate that the TPOFF technique using the DCF probe is an appropriate tool to quantify low nanomolar concentrations of targeted fluorescent probes from deep tissue.",
author = "Thomas, {Thommey P.} and Ye, {Jing Yong} and Chang, {Yu Chung} and Alina Kotlyar and Zhengyi Cao and Majoros, {Istvan J.} and Norris, {Theodore B.} and Baker, {James R.}",
year = "2008",
month = "12",
day = "1",
doi = "10.1117/1.2870105",
language = "English",
volume = "13",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "1",

}

Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. / Thomas, Thommey P.; Ye, Jing Yong; Chang, Yu Chung; Kotlyar, Alina; Cao, Zhengyi; Majoros, Istvan J.; Norris, Theodore B.; Baker, James R.

In: Journal of Biomedical Optics, Vol. 13, No. 1, 014024, 01.12.2008.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe

AU - Thomas, Thommey P.

AU - Ye, Jing Yong

AU - Chang, Yu Chung

AU - Kotlyar, Alina

AU - Cao, Zhengyi

AU - Majoros, Istvan J.

AU - Norris, Theodore B.

AU - Baker, James R.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Fluorescence quantification in tissues using conventional techniques can be difficult due to the absorption and scattering of light in tissues. Our previous studies have shown that a single-mode optical fiber (SMF)-based, two-photon optical fiber fluorescence (TPOFF) probe could be effective as a minimally invasive, real-time technique for quantifying fluorescence in solid tumors. We report improved results with this technique using a solid, double-clad optical fiber (DCF). The DCF can maintain a high excitation rate by propagating ultrashort laser pulses down an inner single-mode core, while demonstrating improved collection efficiency by using a high-numerical aperture multimode outer core confined with a second clad. We have compared the TPOFF detection efficiency of the DCF versus the SMF with standard solutions of the generation 5 poly(amidoamine) dendrimer (G5) nanoparticles G5-6TAMRA (G5-6T) and G5-6TAMRA-folic acid (G5-6T-FA). The DCF probe showed three- to five-fold increases in the detection efficiency of these conjugates, in comparison to the SMF. We also demonstrate the applicability of the DCF to quantify the targeted uptake of G5-6T-FA in mouse tumors expressing the FA receptor. These results indicate that the TPOFF technique using the DCF probe is an appropriate tool to quantify low nanomolar concentrations of targeted fluorescent probes from deep tissue.

AB - Fluorescence quantification in tissues using conventional techniques can be difficult due to the absorption and scattering of light in tissues. Our previous studies have shown that a single-mode optical fiber (SMF)-based, two-photon optical fiber fluorescence (TPOFF) probe could be effective as a minimally invasive, real-time technique for quantifying fluorescence in solid tumors. We report improved results with this technique using a solid, double-clad optical fiber (DCF). The DCF can maintain a high excitation rate by propagating ultrashort laser pulses down an inner single-mode core, while demonstrating improved collection efficiency by using a high-numerical aperture multimode outer core confined with a second clad. We have compared the TPOFF detection efficiency of the DCF versus the SMF with standard solutions of the generation 5 poly(amidoamine) dendrimer (G5) nanoparticles G5-6TAMRA (G5-6T) and G5-6TAMRA-folic acid (G5-6T-FA). The DCF probe showed three- to five-fold increases in the detection efficiency of these conjugates, in comparison to the SMF. We also demonstrate the applicability of the DCF to quantify the targeted uptake of G5-6T-FA in mouse tumors expressing the FA receptor. These results indicate that the TPOFF technique using the DCF probe is an appropriate tool to quantify low nanomolar concentrations of targeted fluorescent probes from deep tissue.

UR - http://www.scopus.com/inward/record.url?scp=44849127014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44849127014&partnerID=8YFLogxK

U2 - 10.1117/1.2870105

DO - 10.1117/1.2870105

M3 - Article

C2 - 18315382

AN - SCOPUS:44849127014

VL - 13

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 1

M1 - 014024

ER -