Inhibitory effect of carnosine and anserine on DNA oxidative damage induced by Fe 2+, Cu 2+ and H 2O 2 in lymphocytes

Chiu-Lan Hsieh, Yi Chun Ho, Hsi Huai Lai, Gow Chin Yen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effects of carnosine and anserine on oxidative DNA damage in human lymphocytes induced by Fe 2+, Cu 2+ or H 2O 2 were investigated using single cell gel electrophoresis (comet assay). Carnosine and anserine induced a slight DNA damage in human lymphocytes after incubation with cells for 30 min. Carnosine and anserine caused a tail DNA % from 4.7% to 7.3% and 6.1% to 10.0% respectively, at a concentration of 5-100 mM. The cell viability of carnosine and anserine to human lymphocytes was more than 85%. Fe 2+ and Cu 2+ caused a marked cytotoxicity and DNA damage in human lymphocytes with a concentration dependent manner. At a concentration of 100 μM, carnosine and anserine possessed 60-70% inhibitory effect on DNA damage in human lymphocytes when they reacted with Fe 2+ or Cu 2+ for 30 min before incubated with human lymphocytes. Fe 2+, Cu 2+, and H 2O 2-induced DNA damage in human lymphocytes were inhibited by carnosine and anserine in a concentration dependent manner (5-100 μM). However, the inhibitory effect of carnosine and anserine on oxidative DNA damage in human lymphocyte was 46.4% and 49.3%, respectively, when reacted simultaneously with H 2O 2. Carnosine and anserine possessed more marked inhibitory effect on oxidative DNA damage in human lymphocyte induced by Fe 2+ and Cu 2+ than that induced by H 2O 2. This result might be due to carnosine and anserine did not possess significantly scavenging effect on H 2O 2, thus, H 2O 2 entered the cell and caused DNA damage.

Original languageEnglish
Pages (from-to)47-54
Number of pages8
JournalJournal of Food and Drug Analysis
Volume10
Issue number1
Publication statusPublished - 2002 Jun 11

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Anserine
anserine
Carnosine
carnosine
DNA Damage
DNA damage
lymphocytes
Lymphocytes
DNA
Comet Assay
cells
gel electrophoresis
cell viability
cytotoxicity
Tail
Cell Survival
tail

All Science Journal Classification (ASJC) codes

  • Food Science
  • Pharmacology

Cite this

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title = "Inhibitory effect of carnosine and anserine on DNA oxidative damage induced by Fe 2+, Cu 2+ and H 2O 2 in lymphocytes",
abstract = "The effects of carnosine and anserine on oxidative DNA damage in human lymphocytes induced by Fe 2+, Cu 2+ or H 2O 2 were investigated using single cell gel electrophoresis (comet assay). Carnosine and anserine induced a slight DNA damage in human lymphocytes after incubation with cells for 30 min. Carnosine and anserine caused a tail DNA {\%} from 4.7{\%} to 7.3{\%} and 6.1{\%} to 10.0{\%} respectively, at a concentration of 5-100 mM. The cell viability of carnosine and anserine to human lymphocytes was more than 85{\%}. Fe 2+ and Cu 2+ caused a marked cytotoxicity and DNA damage in human lymphocytes with a concentration dependent manner. At a concentration of 100 μM, carnosine and anserine possessed 60-70{\%} inhibitory effect on DNA damage in human lymphocytes when they reacted with Fe 2+ or Cu 2+ for 30 min before incubated with human lymphocytes. Fe 2+, Cu 2+, and H 2O 2-induced DNA damage in human lymphocytes were inhibited by carnosine and anserine in a concentration dependent manner (5-100 μM). However, the inhibitory effect of carnosine and anserine on oxidative DNA damage in human lymphocyte was 46.4{\%} and 49.3{\%}, respectively, when reacted simultaneously with H 2O 2. Carnosine and anserine possessed more marked inhibitory effect on oxidative DNA damage in human lymphocyte induced by Fe 2+ and Cu 2+ than that induced by H 2O 2. This result might be due to carnosine and anserine did not possess significantly scavenging effect on H 2O 2, thus, H 2O 2 entered the cell and caused DNA damage.",
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Inhibitory effect of carnosine and anserine on DNA oxidative damage induced by Fe 2+, Cu 2+ and H 2O 2 in lymphocytes. / Hsieh, Chiu-Lan; Ho, Yi Chun; Lai, Hsi Huai; Yen, Gow Chin.

In: Journal of Food and Drug Analysis, Vol. 10, No. 1, 11.06.2002, p. 47-54.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inhibitory effect of carnosine and anserine on DNA oxidative damage induced by Fe 2+, Cu 2+ and H 2O 2 in lymphocytes

AU - Hsieh, Chiu-Lan

AU - Ho, Yi Chun

AU - Lai, Hsi Huai

AU - Yen, Gow Chin

PY - 2002/6/11

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N2 - The effects of carnosine and anserine on oxidative DNA damage in human lymphocytes induced by Fe 2+, Cu 2+ or H 2O 2 were investigated using single cell gel electrophoresis (comet assay). Carnosine and anserine induced a slight DNA damage in human lymphocytes after incubation with cells for 30 min. Carnosine and anserine caused a tail DNA % from 4.7% to 7.3% and 6.1% to 10.0% respectively, at a concentration of 5-100 mM. The cell viability of carnosine and anserine to human lymphocytes was more than 85%. Fe 2+ and Cu 2+ caused a marked cytotoxicity and DNA damage in human lymphocytes with a concentration dependent manner. At a concentration of 100 μM, carnosine and anserine possessed 60-70% inhibitory effect on DNA damage in human lymphocytes when they reacted with Fe 2+ or Cu 2+ for 30 min before incubated with human lymphocytes. Fe 2+, Cu 2+, and H 2O 2-induced DNA damage in human lymphocytes were inhibited by carnosine and anserine in a concentration dependent manner (5-100 μM). However, the inhibitory effect of carnosine and anserine on oxidative DNA damage in human lymphocyte was 46.4% and 49.3%, respectively, when reacted simultaneously with H 2O 2. Carnosine and anserine possessed more marked inhibitory effect on oxidative DNA damage in human lymphocyte induced by Fe 2+ and Cu 2+ than that induced by H 2O 2. This result might be due to carnosine and anserine did not possess significantly scavenging effect on H 2O 2, thus, H 2O 2 entered the cell and caused DNA damage.

AB - The effects of carnosine and anserine on oxidative DNA damage in human lymphocytes induced by Fe 2+, Cu 2+ or H 2O 2 were investigated using single cell gel electrophoresis (comet assay). Carnosine and anserine induced a slight DNA damage in human lymphocytes after incubation with cells for 30 min. Carnosine and anserine caused a tail DNA % from 4.7% to 7.3% and 6.1% to 10.0% respectively, at a concentration of 5-100 mM. The cell viability of carnosine and anserine to human lymphocytes was more than 85%. Fe 2+ and Cu 2+ caused a marked cytotoxicity and DNA damage in human lymphocytes with a concentration dependent manner. At a concentration of 100 μM, carnosine and anserine possessed 60-70% inhibitory effect on DNA damage in human lymphocytes when they reacted with Fe 2+ or Cu 2+ for 30 min before incubated with human lymphocytes. Fe 2+, Cu 2+, and H 2O 2-induced DNA damage in human lymphocytes were inhibited by carnosine and anserine in a concentration dependent manner (5-100 μM). However, the inhibitory effect of carnosine and anserine on oxidative DNA damage in human lymphocyte was 46.4% and 49.3%, respectively, when reacted simultaneously with H 2O 2. Carnosine and anserine possessed more marked inhibitory effect on oxidative DNA damage in human lymphocyte induced by Fe 2+ and Cu 2+ than that induced by H 2O 2. This result might be due to carnosine and anserine did not possess significantly scavenging effect on H 2O 2, thus, H 2O 2 entered the cell and caused DNA damage.

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