Influence of ions, hydration, and the transcriptional inhibitor P4N on the conformations of the Sp1 binding site

Julie A. Dohm, Ming-Hua Hsu, Jih Ru Hwu, Ru Chih C. Huang, Evangelos N. Moudrianakis, Eaton E. Lattman, Apostolos G. Gittis

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Three crystal structures containing the entire Sp1 consensus sequence d(GGGGCGGGG) with two or three additional base-pairs on either the 5′ or 3′ ends and overhangs have been determined. Despite the different lengths of DNA in the pseudo-dodecamers and pseudo-tridecamer, all three structures form A-DNA duplexes that share a common set of crystal contacts, including a T*(G·C) base triplet and a 5′-overhang that flips out and away from the helical axes to form a Hoogsteen base-pair with the 3′-overhang of a symmetry mate. The global conformations of the three structures differ, however, in the widths of their respective major grooves, the lengths of the molecules, and the extent of crystal packing. The structures were determined from crystals grown in an unusual precipitant for A-DNA, polyethylene glycol (PEG) 400, in combination with polyamines or ions; cobalt hexamine for the pseudo-tridecamer, and spermidine for the pseudo-dodecamers. As the Sp1 binding site is a target for antiviral and anticancer drugs, pseudo-dodecamer crystals were soaked with one such antiviral and anticancer compound, P4N. Although P4N was not visualized unambiguously in the electron density maps, the effect of the drug is evident from significant differences in the lattice constants, crystal packing, and overall conformation of the structure.

Original languageEnglish
Pages (from-to)731-744
Number of pages14
JournalJournal of Molecular Biology
Volume349
Issue number4
DOIs
Publication statusPublished - 2005 Jun 17

Fingerprint

Base Pairing
Antiviral Agents
A-Form DNA
Binding Sites
Methenamine
Ions
Spermidine
DNA
Consensus Sequence
Polyamines
Cobalt
Electrons
Pharmaceutical Preparations
polyethylene glycol 400

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Dohm, Julie A. ; Hsu, Ming-Hua ; Hwu, Jih Ru ; Huang, Ru Chih C. ; Moudrianakis, Evangelos N. ; Lattman, Eaton E. ; Gittis, Apostolos G. / Influence of ions, hydration, and the transcriptional inhibitor P4N on the conformations of the Sp1 binding site. In: Journal of Molecular Biology. 2005 ; Vol. 349, No. 4. pp. 731-744.
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Influence of ions, hydration, and the transcriptional inhibitor P4N on the conformations of the Sp1 binding site. / Dohm, Julie A.; Hsu, Ming-Hua; Hwu, Jih Ru; Huang, Ru Chih C.; Moudrianakis, Evangelos N.; Lattman, Eaton E.; Gittis, Apostolos G.

In: Journal of Molecular Biology, Vol. 349, No. 4, 17.06.2005, p. 731-744.

Research output: Contribution to journalArticle

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