Induction of glutathione S-transferases activities in Drosophila melanogaster exposed to phenol

S. Shen, Yi-Chih Chien, C. Chien

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Studying the toxic effects of long-term exposing fruit flies to phenol is the object of this study. The induction of the glutathione S-transferases enzymatic activities, the change in the amount of mRNA related to phenol exposure, the change in survival rate of adult fruit flies, and the chemical interaction between phenol and benzene were the problems to be investigated. Glutathione S-transferases were separated by affinity chromatography and the mRNAs levels were quantified by reverse-transcription polymerase chain reaction. Long-term feeding phenol to wild type fruit flies had caused some toxic effects included increasing the resistance to phenol toxicity, lowering the benzene toxicity, and induction of glutathione S-transferases enzymatic activities. But no significant change in the amount of glutathione S-transferases GstD1 and GstD5 mRNAs had occurred. From these results, we concluded that fruit flies could develop resistance to phenol by decreasing its toxicity; phenol was a inducer of glutathione S-transferases; phenol could increase the glutathione S-transferases enzymatic activities by increasing the amount of proteins; phenol exposure could decrease the benzene toxicity; no new glutathione S-transferase isozyme subunit was induced; and the level of GstD1 and GstD5 mRNAs did not significantly increase in phenol-treated strain.

Original languageEnglish
Pages (from-to)80-91
Number of pages12
JournalArchives of Insect Biochemistry and Physiology
Volume53
Issue number2
DOIs
Publication statusPublished - 2003 Jun 1

Fingerprint

Phenol
Glutathione Transferase
Drosophila melanogaster
glutathione transferase
phenol
fruit flies
Fruits
Diptera
Toxicity
Fruit
benzene
Benzene
toxicity
Messenger RNA
Poisons
Affinity chromatography
chemical interactions
Polymerase chain reaction
Transcription
affinity chromatography

All Science Journal Classification (ASJC) codes

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Physiology (medical)

Cite this

@article{2c70ab4657294906ae45467992134141,
title = "Induction of glutathione S-transferases activities in Drosophila melanogaster exposed to phenol",
abstract = "Studying the toxic effects of long-term exposing fruit flies to phenol is the object of this study. The induction of the glutathione S-transferases enzymatic activities, the change in the amount of mRNA related to phenol exposure, the change in survival rate of adult fruit flies, and the chemical interaction between phenol and benzene were the problems to be investigated. Glutathione S-transferases were separated by affinity chromatography and the mRNAs levels were quantified by reverse-transcription polymerase chain reaction. Long-term feeding phenol to wild type fruit flies had caused some toxic effects included increasing the resistance to phenol toxicity, lowering the benzene toxicity, and induction of glutathione S-transferases enzymatic activities. But no significant change in the amount of glutathione S-transferases GstD1 and GstD5 mRNAs had occurred. From these results, we concluded that fruit flies could develop resistance to phenol by decreasing its toxicity; phenol was a inducer of glutathione S-transferases; phenol could increase the glutathione S-transferases enzymatic activities by increasing the amount of proteins; phenol exposure could decrease the benzene toxicity; no new glutathione S-transferase isozyme subunit was induced; and the level of GstD1 and GstD5 mRNAs did not significantly increase in phenol-treated strain.",
author = "S. Shen and Yi-Chih Chien and C. Chien",
year = "2003",
month = "6",
day = "1",
doi = "10.1002/arch.10087",
language = "English",
volume = "53",
pages = "80--91",
journal = "Archives of Insect Biochemistry and Physiology",
issn = "0739-4462",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

Induction of glutathione S-transferases activities in Drosophila melanogaster exposed to phenol. / Shen, S.; Chien, Yi-Chih; Chien, C.

In: Archives of Insect Biochemistry and Physiology, Vol. 53, No. 2, 01.06.2003, p. 80-91.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Induction of glutathione S-transferases activities in Drosophila melanogaster exposed to phenol

AU - Shen, S.

AU - Chien, Yi-Chih

AU - Chien, C.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - Studying the toxic effects of long-term exposing fruit flies to phenol is the object of this study. The induction of the glutathione S-transferases enzymatic activities, the change in the amount of mRNA related to phenol exposure, the change in survival rate of adult fruit flies, and the chemical interaction between phenol and benzene were the problems to be investigated. Glutathione S-transferases were separated by affinity chromatography and the mRNAs levels were quantified by reverse-transcription polymerase chain reaction. Long-term feeding phenol to wild type fruit flies had caused some toxic effects included increasing the resistance to phenol toxicity, lowering the benzene toxicity, and induction of glutathione S-transferases enzymatic activities. But no significant change in the amount of glutathione S-transferases GstD1 and GstD5 mRNAs had occurred. From these results, we concluded that fruit flies could develop resistance to phenol by decreasing its toxicity; phenol was a inducer of glutathione S-transferases; phenol could increase the glutathione S-transferases enzymatic activities by increasing the amount of proteins; phenol exposure could decrease the benzene toxicity; no new glutathione S-transferase isozyme subunit was induced; and the level of GstD1 and GstD5 mRNAs did not significantly increase in phenol-treated strain.

AB - Studying the toxic effects of long-term exposing fruit flies to phenol is the object of this study. The induction of the glutathione S-transferases enzymatic activities, the change in the amount of mRNA related to phenol exposure, the change in survival rate of adult fruit flies, and the chemical interaction between phenol and benzene were the problems to be investigated. Glutathione S-transferases were separated by affinity chromatography and the mRNAs levels were quantified by reverse-transcription polymerase chain reaction. Long-term feeding phenol to wild type fruit flies had caused some toxic effects included increasing the resistance to phenol toxicity, lowering the benzene toxicity, and induction of glutathione S-transferases enzymatic activities. But no significant change in the amount of glutathione S-transferases GstD1 and GstD5 mRNAs had occurred. From these results, we concluded that fruit flies could develop resistance to phenol by decreasing its toxicity; phenol was a inducer of glutathione S-transferases; phenol could increase the glutathione S-transferases enzymatic activities by increasing the amount of proteins; phenol exposure could decrease the benzene toxicity; no new glutathione S-transferase isozyme subunit was induced; and the level of GstD1 and GstD5 mRNAs did not significantly increase in phenol-treated strain.

UR - http://www.scopus.com/inward/record.url?scp=0037591126&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037591126&partnerID=8YFLogxK

U2 - 10.1002/arch.10087

DO - 10.1002/arch.10087

M3 - Article

C2 - 12761875

AN - SCOPUS:0037591126

VL - 53

SP - 80

EP - 91

JO - Archives of Insect Biochemistry and Physiology

JF - Archives of Insect Biochemistry and Physiology

SN - 0739-4462

IS - 2

ER -