Indole-3-acetic acid-producing yeasts in the phyllosphere of the carnivorous plant Drosera indica L

Pei Feng Sun, Wei Ta Fang, Li Ying Shin, Jyuan Yu Wei, Shih-Feng Fu, Jui-Yu Chou

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Yeasts are widely distributed in nature and exist in association with other microorganisms as normal inhabitants of soil, vegetation, and aqueous environments. In this study, 12 yeast strains were enriched and isolated from leaf samples of the carnivorous plant Drosera indica L., which is currently threatened because of restricted habitats and use in herbal industries. According to similarities in large subunit and small subunit ribosomal RNA gene sequences, we identified 2 yeast species in 2 genera of the phylum Ascomycota, and 5 yeast species in 5 genera of the phylum Basidiomycota. All of the isolated yeasts produced indole-3-acetic acid (IAA) when cultivated in YPD broth supplemented with 0.1% L-tryptophan. Growth conditions, such as the pH and temperature of the medium, influenced yeast IAA production. Our results also suggested the existence of a tryptophan-independent IAA biosynthetic pathway. We evaluated the effects of various concentrations of exogenous IAA on yeast growth and observed that IAA produced by wild yeasts modifies auxin-inducible gene expression in Arabidopsis. Our data suggest that yeasts can promote plant growth and support ongoing prospecting of yeast strains for inclusion into biofertilizer for sustainable agriculture.

Original languageEnglish
Article numbere114196
JournalPLoS One
Volume9
Issue number12
DOIs
Publication statusPublished - 2014 Dec 2

Fingerprint

Drosera
carnivorous plants
phyllosphere
Yeast
indole acetic acid
Yeasts
yeasts
Tryptophan
tryptophan
Growth
indoleacetic acid
Basidiomycota
Ascomycota
Indoleacetic Acids
Ribosomal RNA
biofertilizers
Biosynthetic Pathways
sustainable agriculture
Agriculture
rRNA Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sun, Pei Feng ; Fang, Wei Ta ; Shin, Li Ying ; Wei, Jyuan Yu ; Fu, Shih-Feng ; Chou, Jui-Yu. / Indole-3-acetic acid-producing yeasts in the phyllosphere of the carnivorous plant Drosera indica L. In: PLoS One. 2014 ; Vol. 9, No. 12.
@article{72013fca8cda4b3f86352810488e9518,
title = "Indole-3-acetic acid-producing yeasts in the phyllosphere of the carnivorous plant Drosera indica L",
abstract = "Yeasts are widely distributed in nature and exist in association with other microorganisms as normal inhabitants of soil, vegetation, and aqueous environments. In this study, 12 yeast strains were enriched and isolated from leaf samples of the carnivorous plant Drosera indica L., which is currently threatened because of restricted habitats and use in herbal industries. According to similarities in large subunit and small subunit ribosomal RNA gene sequences, we identified 2 yeast species in 2 genera of the phylum Ascomycota, and 5 yeast species in 5 genera of the phylum Basidiomycota. All of the isolated yeasts produced indole-3-acetic acid (IAA) when cultivated in YPD broth supplemented with 0.1{\%} L-tryptophan. Growth conditions, such as the pH and temperature of the medium, influenced yeast IAA production. Our results also suggested the existence of a tryptophan-independent IAA biosynthetic pathway. We evaluated the effects of various concentrations of exogenous IAA on yeast growth and observed that IAA produced by wild yeasts modifies auxin-inducible gene expression in Arabidopsis. Our data suggest that yeasts can promote plant growth and support ongoing prospecting of yeast strains for inclusion into biofertilizer for sustainable agriculture.",
author = "Sun, {Pei Feng} and Fang, {Wei Ta} and Shin, {Li Ying} and Wei, {Jyuan Yu} and Shih-Feng Fu and Jui-Yu Chou",
year = "2014",
month = "12",
day = "2",
doi = "10.1371/journal.pone.0114196",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

Indole-3-acetic acid-producing yeasts in the phyllosphere of the carnivorous plant Drosera indica L. / Sun, Pei Feng; Fang, Wei Ta; Shin, Li Ying; Wei, Jyuan Yu; Fu, Shih-Feng; Chou, Jui-Yu.

In: PLoS One, Vol. 9, No. 12, e114196, 02.12.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Indole-3-acetic acid-producing yeasts in the phyllosphere of the carnivorous plant Drosera indica L

AU - Sun, Pei Feng

AU - Fang, Wei Ta

AU - Shin, Li Ying

AU - Wei, Jyuan Yu

AU - Fu, Shih-Feng

AU - Chou, Jui-Yu

PY - 2014/12/2

Y1 - 2014/12/2

N2 - Yeasts are widely distributed in nature and exist in association with other microorganisms as normal inhabitants of soil, vegetation, and aqueous environments. In this study, 12 yeast strains were enriched and isolated from leaf samples of the carnivorous plant Drosera indica L., which is currently threatened because of restricted habitats and use in herbal industries. According to similarities in large subunit and small subunit ribosomal RNA gene sequences, we identified 2 yeast species in 2 genera of the phylum Ascomycota, and 5 yeast species in 5 genera of the phylum Basidiomycota. All of the isolated yeasts produced indole-3-acetic acid (IAA) when cultivated in YPD broth supplemented with 0.1% L-tryptophan. Growth conditions, such as the pH and temperature of the medium, influenced yeast IAA production. Our results also suggested the existence of a tryptophan-independent IAA biosynthetic pathway. We evaluated the effects of various concentrations of exogenous IAA on yeast growth and observed that IAA produced by wild yeasts modifies auxin-inducible gene expression in Arabidopsis. Our data suggest that yeasts can promote plant growth and support ongoing prospecting of yeast strains for inclusion into biofertilizer for sustainable agriculture.

AB - Yeasts are widely distributed in nature and exist in association with other microorganisms as normal inhabitants of soil, vegetation, and aqueous environments. In this study, 12 yeast strains were enriched and isolated from leaf samples of the carnivorous plant Drosera indica L., which is currently threatened because of restricted habitats and use in herbal industries. According to similarities in large subunit and small subunit ribosomal RNA gene sequences, we identified 2 yeast species in 2 genera of the phylum Ascomycota, and 5 yeast species in 5 genera of the phylum Basidiomycota. All of the isolated yeasts produced indole-3-acetic acid (IAA) when cultivated in YPD broth supplemented with 0.1% L-tryptophan. Growth conditions, such as the pH and temperature of the medium, influenced yeast IAA production. Our results also suggested the existence of a tryptophan-independent IAA biosynthetic pathway. We evaluated the effects of various concentrations of exogenous IAA on yeast growth and observed that IAA produced by wild yeasts modifies auxin-inducible gene expression in Arabidopsis. Our data suggest that yeasts can promote plant growth and support ongoing prospecting of yeast strains for inclusion into biofertilizer for sustainable agriculture.

UR - http://www.scopus.com/inward/record.url?scp=84914689046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84914689046&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0114196

DO - 10.1371/journal.pone.0114196

M3 - Article

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e114196

ER -