Incompatibility of Nuclear and Mitochondrial Genomes Causes Hybrid Sterility between Two Yeast Species

Hsin Yi Lee, Jui-Yu Chou, Liplee Cheong, Nai Hsin Chang, Shi Yow Yang, Jun Yi Leu

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Hybrids between species are usually unviable or sterile. One possible mechanism causing reproductive isolation is incompatibility between genes from different species. These "speciation" genes are interacting components that cannot function properly when mixed with alleles from other species. To test whether such genes exist in two closely related yeast species, we constructed hybrid lines in which one or two chromosomes were derived from Saccharomyces bayanus, and the rest were from Saccharomyces cerevisiae. We found that the hybrid line with Chromosome 13 substitution was completely sterile and identified Aep2, a mitochondrial protein encoded on Chromosome 13, to cause the sporulation defect as S. bayanus AEP2 is incompatible with S. cerevisiae mitochondria. This is caused by the inability of S. bayanus Aep2 protein to regulate the translation of the S. cerevisiae OLI1 mRNA. We speculate that AEP2 and OLI1 have evolved during the adaptation of S. bayanus to nonfermentable carbon sources, thereby driving speciation.

Original languageEnglish
Pages (from-to)1065-1073
Number of pages9
JournalCell
Volume135
Issue number6
DOIs
Publication statusPublished - 2008 Dec 12

Fingerprint

Mitochondrial Genome
Yeast
Infertility
Saccharomyces cerevisiae
Chromosomes, Human, Pair 13
Chromosomes
Genes
Yeasts
Reproductive Isolation
Gene Components
Saccharomyces
Mitochondrial Proteins
Mitochondria
Carbon
Alleles
Messenger RNA
Substitution reactions
Defects
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lee, Hsin Yi ; Chou, Jui-Yu ; Cheong, Liplee ; Chang, Nai Hsin ; Yang, Shi Yow ; Leu, Jun Yi. / Incompatibility of Nuclear and Mitochondrial Genomes Causes Hybrid Sterility between Two Yeast Species. In: Cell. 2008 ; Vol. 135, No. 6. pp. 1065-1073.
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Incompatibility of Nuclear and Mitochondrial Genomes Causes Hybrid Sterility between Two Yeast Species. / Lee, Hsin Yi; Chou, Jui-Yu; Cheong, Liplee; Chang, Nai Hsin; Yang, Shi Yow; Leu, Jun Yi.

In: Cell, Vol. 135, No. 6, 12.12.2008, p. 1065-1073.

Research output: Contribution to journalArticle

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