In vitro oxidized and glycated human low-density lipoprotein particles characterized by capillary zone electrophoresis

Jing Huei Chiu, Yu Nong Peng, Ying Ling Yang, Ming Hua Tsai, Yu Ling Ho, Chung Yu Wu, Mine-Yine Liu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A simple capillary zone electrophoresis (CZE) method was used to determine native, in vitro Cu2+ and glucose modified low-density lipoprotein (LDL) particles for four healthy subjects. The LDL electropherograms are highly reproducible with good precisions of effective mobility and peak area. The native LDL capillary electrophoresis (CE) profile shows a major peak with lower mobility and two minor peaks with higher mobilities. For three-hour Cu2+ oxidation, one major peak with mobility close to that of the native major peak, and one minor peak with mobility extending to -47 × 10-5 cm2 V-1 s-1 appear. For eighteen-hour Cu2+ oxidation, one major peak with mobility much higher than that of the native major peak appears. As the reaction time for LDL and Cu2+ increases from 0 to 24 h, effective mobility of the LDL major peak increases, suggesting that LDL particles become more negatively charged and oxidized as the time increases. The in vitro glycated LDL particles are characterized by a major peak and two minor peaks. Mobility of the major peak is close to that of native major peak, but the second minor peak is much more negatively charged with mobility extending to -53 × 10-5 cm2 V-1 s-1. Native, oxidized and glycated LDL particles show distinctive differences in their CZE profiles. Agarose electrophoresis shows that the charge to mass ratios of native, three-hour Cu2+ and glucose modified LDL particles are similar, but that of eighteen-hour Cu2+ oxidized LDL particles is higher.

Original languageEnglish
Pages (from-to)383-391
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume875
Issue number2
DOIs
Publication statusPublished - 2008 Nov 15

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Capillary Electrophoresis
Electrophoresis
LDL Lipoproteins
Glucose
In Vitro Techniques
Capillary electrophoresis
Oxidation
Sepharose
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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title = "In vitro oxidized and glycated human low-density lipoprotein particles characterized by capillary zone electrophoresis",
abstract = "A simple capillary zone electrophoresis (CZE) method was used to determine native, in vitro Cu2+ and glucose modified low-density lipoprotein (LDL) particles for four healthy subjects. The LDL electropherograms are highly reproducible with good precisions of effective mobility and peak area. The native LDL capillary electrophoresis (CE) profile shows a major peak with lower mobility and two minor peaks with higher mobilities. For three-hour Cu2+ oxidation, one major peak with mobility close to that of the native major peak, and one minor peak with mobility extending to -47 × 10-5 cm2 V-1 s-1 appear. For eighteen-hour Cu2+ oxidation, one major peak with mobility much higher than that of the native major peak appears. As the reaction time for LDL and Cu2+ increases from 0 to 24 h, effective mobility of the LDL major peak increases, suggesting that LDL particles become more negatively charged and oxidized as the time increases. The in vitro glycated LDL particles are characterized by a major peak and two minor peaks. Mobility of the major peak is close to that of native major peak, but the second minor peak is much more negatively charged with mobility extending to -53 × 10-5 cm2 V-1 s-1. Native, oxidized and glycated LDL particles show distinctive differences in their CZE profiles. Agarose electrophoresis shows that the charge to mass ratios of native, three-hour Cu2+ and glucose modified LDL particles are similar, but that of eighteen-hour Cu2+ oxidized LDL particles is higher.",
author = "Chiu, {Jing Huei} and Peng, {Yu Nong} and Yang, {Ying Ling} and Tsai, {Ming Hua} and Ho, {Yu Ling} and Wu, {Chung Yu} and Mine-Yine Liu",
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In vitro oxidized and glycated human low-density lipoprotein particles characterized by capillary zone electrophoresis. / Chiu, Jing Huei; Peng, Yu Nong; Yang, Ying Ling; Tsai, Ming Hua; Ho, Yu Ling; Wu, Chung Yu; Liu, Mine-Yine.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 875, No. 2, 15.11.2008, p. 383-391.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vitro oxidized and glycated human low-density lipoprotein particles characterized by capillary zone electrophoresis

AU - Chiu, Jing Huei

AU - Peng, Yu Nong

AU - Yang, Ying Ling

AU - Tsai, Ming Hua

AU - Ho, Yu Ling

AU - Wu, Chung Yu

AU - Liu, Mine-Yine

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