Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de Novo Approach: Size-Selective Sheltering of Catalase in Metal-Organic Framework Microcrystals

Fa Kuen Shieh, Shao Chun Wang, Chia I. Yen, Chang Cheng Wu, Saikat Dutta, Lien Yang Chou, Joseph V. Morabito, Pan Hu, Ming Hua Hsu, Kevin C.W. Wu, Chia Kuang Tsung

Research output: Contribution to journalArticle

368 Citations (Scopus)


We develop a new concept to impart new functions to biocatalysts by combining enzymes and metal-organic frameworks (MOFs). The proof-of-concept design is demonstrated by embedding catalase molecules into uniformly sized ZIF-90 crystals via a de novo approach. We have carried out electron microscopy, X-ray diffraction, nitrogen sorption, electrophoresis, thermogravimetric analysis, and confocal microscopy to confirm that the ∼10 nm catalase molecules are embedded in 2 μm single-crystalline ZIF-90 crystals with ∼5 wt % loading. Because catalase is immobilized and sheltered by the ZIF-90 crystals, the composites show activity in hydrogen peroxide degradation even in the presence of protease proteinase K.

Original languageEnglish
Pages (from-to)4276-4279
Number of pages4
JournalJournal of the American Chemical Society
Issue number13
Publication statusPublished - 2015 Apr 8


All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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