Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de Novo Approach: Size-Selective Sheltering of Catalase in Metal-Organic Framework Microcrystals

Fa Kuen Shieh, Shao Chun Wang, Chia I. Yen, Chang Cheng Wu, Saikat Dutta, Lien Yang Chou, Joseph V. Morabito, Pan Hu, Ming-Hua Hsu, Kevin C.W. Wu, Chia Kuang Tsung

Research output: Contribution to journalArticle

313 Citations (Scopus)

Abstract

We develop a new concept to impart new functions to biocatalysts by combining enzymes and metal-organic frameworks (MOFs). The proof-of-concept design is demonstrated by embedding catalase molecules into uniformly sized ZIF-90 crystals via a de novo approach. We have carried out electron microscopy, X-ray diffraction, nitrogen sorption, electrophoresis, thermogravimetric analysis, and confocal microscopy to confirm that the ∼10 nm catalase molecules are embedded in 2 μm single-crystalline ZIF-90 crystals with ∼5 wt % loading. Because catalase is immobilized and sheltered by the ZIF-90 crystals, the composites show activity in hydrogen peroxide degradation even in the presence of protease proteinase K.

Original languageEnglish
Pages (from-to)4276-4279
Number of pages4
JournalJournal of the American Chemical Society
Volume137
Issue number13
DOIs
Publication statusPublished - 2015 Apr 8

Fingerprint

Microcrystals
Biocatalysts
Catalase
Endopeptidase K
Enzymes
Metals
Crystals
Molecules
Confocal microscopy
Electrophoresis
Confocal Microscopy
Hydrogen peroxide
X-Ray Diffraction
Hydrogen Peroxide
Electron microscopy
Thermogravimetric analysis
Sorption
Electron Microscopy
Peptide Hydrolases
Nitrogen

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Shieh, Fa Kuen ; Wang, Shao Chun ; Yen, Chia I. ; Wu, Chang Cheng ; Dutta, Saikat ; Chou, Lien Yang ; Morabito, Joseph V. ; Hu, Pan ; Hsu, Ming-Hua ; Wu, Kevin C.W. ; Tsung, Chia Kuang. / Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de Novo Approach : Size-Selective Sheltering of Catalase in Metal-Organic Framework Microcrystals. In: Journal of the American Chemical Society. 2015 ; Vol. 137, No. 13. pp. 4276-4279.
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Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de Novo Approach : Size-Selective Sheltering of Catalase in Metal-Organic Framework Microcrystals. / Shieh, Fa Kuen; Wang, Shao Chun; Yen, Chia I.; Wu, Chang Cheng; Dutta, Saikat; Chou, Lien Yang; Morabito, Joseph V.; Hu, Pan; Hsu, Ming-Hua; Wu, Kevin C.W.; Tsung, Chia Kuang.

In: Journal of the American Chemical Society, Vol. 137, No. 13, 08.04.2015, p. 4276-4279.

Research output: Contribution to journalArticle

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AU - Morabito, Joseph V.

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AU - Tsung, Chia Kuang

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