Identification of a novel septin 4 protein binding to human herpesvirus 8 kaposin A protein using a phage display cDNA library

Cheng Wen Lin, Ping Feng Tu, Nai-Wan Hsiao, Ching Yao Chang, Lei Wan, Yu Tsun Lin, Hsueh Wei Chang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Human herpesvirus 8 (HHV-8) is associated with the development of Kaposi's sarcoma and several other human malignancies. Kaposin A protein of HHV-8 has been demonstrated as inducing tumorigenic transformation, being responsible for nuclear receptor coactivators in the transforming activity. In this study, a kaposin A-interacting septin 4 variant that contained the unique GDR at the N-terminus and AAALE at the C-terminus was identified using affinity selection of a phage display library. Co-immunoprecipitation and confocal imaging revealed in vitro binding specificity and in vivo co-localization of HHV-8 kaposin A protein to the septin 4 variant. The kaposin A-interacting septin 4 variant induced cell rounding up, activated caspase-3, and up-regulated transcriptional factor NF-κB. By contrast, kaposin A protein showed an antagonistic effect on the biological functions of the septin 4 variant. Therefore, the interaction of kaposin A protein and the septin 4 variant was suggested as playing a possible role in the development of HHV-8-associated malignancies. This study provides insights into the mechanism of the kaposin A protein pathology, in which the interactions of kaposin A protein with cellular proteins might allow alteration of fundamental cellular processes.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalJournal of Virological Methods
Volume143
Issue number1
DOIs
Publication statusPublished - 2007 Jul 1

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Septins
Gene Library
Protein Binding
Bacteriophages
Human Herpesvirus 8
Proteins
Nuclear Receptor Coactivators
Neoplastic Cell Transformation
Kaposi's Sarcoma
Immunoprecipitation
Caspase 3
Libraries
Neoplasms
Human herpesvirus 8 kaposin B protein
Pathology

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Lin, Cheng Wen ; Tu, Ping Feng ; Hsiao, Nai-Wan ; Chang, Ching Yao ; Wan, Lei ; Lin, Yu Tsun ; Chang, Hsueh Wei. / Identification of a novel septin 4 protein binding to human herpesvirus 8 kaposin A protein using a phage display cDNA library. In: Journal of Virological Methods. 2007 ; Vol. 143, No. 1. pp. 65-72.
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Identification of a novel septin 4 protein binding to human herpesvirus 8 kaposin A protein using a phage display cDNA library. / Lin, Cheng Wen; Tu, Ping Feng; Hsiao, Nai-Wan; Chang, Ching Yao; Wan, Lei; Lin, Yu Tsun; Chang, Hsueh Wei.

In: Journal of Virological Methods, Vol. 143, No. 1, 01.07.2007, p. 65-72.

Research output: Contribution to journalArticle

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