Abstract
DNA methylation is essential for normal developmental processes and genome stability. DNA methyltransferases are key enzymes catalyzing DNA methylation. Chromomethylase (CMT) genes are specific to the plant kingdom and encode chromodomain-containing methyltransferases. However, the function of CMT genes in plants remains elusive. In this study, we isolated and characterized a CMT gene from Nicotiana benthamiana, designated NbCMT3. Alignment of the NbCMT3 amino acid sequence with other plant CMT3s showed conservation of bromo-adjacent-homology and methyltransferase catalytic domains. We investigated the expression patterns of NbCMT3 and its function in developmental programs. NbCMT3 was expressed predominately in proliferating tissues such as apical shoots and young leaves. NbCMT3 protein showed a nuclear location, which could be related to its putative cellular functions. Knocking down NbCMT3 expression by virus-induced gene silencing revealed its vital role(s) in leaf morphogenesis. The formation of palisade cells was defective in NbCMT3-silenced plants as compared with controls. NbCMT3 has a role in developmental programs.
| Original language | English |
|---|---|
| Pages (from-to) | 119-132 |
| Number of pages | 14 |
| Journal | Physiologia Plantarum |
| Volume | 150 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Genetics
- Plant Science
- Cell Biology
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Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus-induced gene silencing. / Hou, Pin Quan; Lee, Yung I.; Hsu, Kuei Ting; Lin, Yu Ting; Wu, Wei Zhi; Lin, John Yee; Nam, Trinh Ngoc; Fu, Shih Feng.
In: Physiologia Plantarum, Vol. 150, No. 1, 01.01.2014, p. 119-132.Research output: Contribution to journal › Article
TY - JOUR
T1 - Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus-induced gene silencing
AU - Hou, Pin Quan
AU - Lee, Yung I.
AU - Hsu, Kuei Ting
AU - Lin, Yu Ting
AU - Wu, Wei Zhi
AU - Lin, John Yee
AU - Nam, Trinh Ngoc
AU - Fu, Shih Feng
PY - 2014/1/1
Y1 - 2014/1/1
N2 - DNA methylation is essential for normal developmental processes and genome stability. DNA methyltransferases are key enzymes catalyzing DNA methylation. Chromomethylase (CMT) genes are specific to the plant kingdom and encode chromodomain-containing methyltransferases. However, the function of CMT genes in plants remains elusive. In this study, we isolated and characterized a CMT gene from Nicotiana benthamiana, designated NbCMT3. Alignment of the NbCMT3 amino acid sequence with other plant CMT3s showed conservation of bromo-adjacent-homology and methyltransferase catalytic domains. We investigated the expression patterns of NbCMT3 and its function in developmental programs. NbCMT3 was expressed predominately in proliferating tissues such as apical shoots and young leaves. NbCMT3 protein showed a nuclear location, which could be related to its putative cellular functions. Knocking down NbCMT3 expression by virus-induced gene silencing revealed its vital role(s) in leaf morphogenesis. The formation of palisade cells was defective in NbCMT3-silenced plants as compared with controls. NbCMT3 has a role in developmental programs.
AB - DNA methylation is essential for normal developmental processes and genome stability. DNA methyltransferases are key enzymes catalyzing DNA methylation. Chromomethylase (CMT) genes are specific to the plant kingdom and encode chromodomain-containing methyltransferases. However, the function of CMT genes in plants remains elusive. In this study, we isolated and characterized a CMT gene from Nicotiana benthamiana, designated NbCMT3. Alignment of the NbCMT3 amino acid sequence with other plant CMT3s showed conservation of bromo-adjacent-homology and methyltransferase catalytic domains. We investigated the expression patterns of NbCMT3 and its function in developmental programs. NbCMT3 was expressed predominately in proliferating tissues such as apical shoots and young leaves. NbCMT3 protein showed a nuclear location, which could be related to its putative cellular functions. Knocking down NbCMT3 expression by virus-induced gene silencing revealed its vital role(s) in leaf morphogenesis. The formation of palisade cells was defective in NbCMT3-silenced plants as compared with controls. NbCMT3 has a role in developmental programs.
UR - http://www.scopus.com/inward/record.url?scp=84890433614&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890433614&partnerID=8YFLogxK
U2 - 10.1111/ppl.12071
DO - 10.1111/ppl.12071
M3 - Article
C2 - 23683172
AN - SCOPUS:84890433614
VL - 150
SP - 119
EP - 132
JO - Physiologia Plantarum
JF - Physiologia Plantarum
SN - 0031-9317
IS - 1
ER -