Functional assessment of residues in the amino-and carboxyl-termini of crustacean hyperglycemic hormone (CHH) in the mud crab scylla olivacea using point-mutated peptides

Chun Jing Liu, Shiau Shan Huang, Jean Yves Toullec, Cheng Yen Chang, Yun Ru Chen, Wen San Huang, Chi Ying Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To assess functional importance of the residues in the amino-and carboxyl-termini of crustacean hyperglycemic hormone in the mud crab Scylla olivacea (Sco-CHH), both wild-type and point-mutated CHH peptides were produced with an amidated C-terminal end. Spectral analyses of circular dichroism, chromatographic retention time, and mass spectrometric analysis of the recombinant peptides indicate that they were close in conformation to native CHH and were produced with the intended substitutions. The recombinant peptides were subsequently used for an in vivo hyperglycemic assay. Two mutants (R13A and I69A rSco-CHH) completely lacked hyperglycemic activity, with temporal profiles similar to that of vehicle control. Temporal profiles of hyperglycemic responses elicited by 4 mutants (I2A, F3A, D12A, and D60A Sco-CHH) were different from that elicited by wild-type Sco-CHH; I2A was unique in that it exhibited significantly higher hyperglycemic activity, whereas the remaining 3 mutants showed lower activity. Four mutants (D4A, Q51A, E54A, and V72A rSco-CHH) elicited hyperglycemic responses with temporal profiles similar to those evoked by wild-type Sco-CHH. In contrast, the glycine-extended version of V72A rSco-CHH (V72A rSco-CHHGly) completely lost hyperglycemic activity. By comparing our study with previous ones of ion-transport peptide (ITP) and molt-inhibiting hormone (MIH) using deleted or point-mutated mutants, detail discussion is made regarding functionally important residues that are shared by both CHH and ITP (members of Group I of the CHH family), and those that discriminate CHH from ITP, and Group-I from Group-II peptides. Conclusions summarized in the present study provide insights into understanding of how functional diversification occurred within a peptide family of multifunctional members.

Original languageEnglish
Article numbere0134983
JournalPLoS ONE
Volume10
Issue number8
DOIs
Publication statusPublished - 2015 Aug 11

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Scylla olivacea
Functional assessment
crabs
Crustacea
hormones
peptides
Peptides
glycemic effect
Ion Transport
ion transport
mutants
Ions
crustacean hyperglycemic hormone
circular dichroism spectroscopy
peptide hormones

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Liu, Chun Jing ; Huang, Shiau Shan ; Toullec, Jean Yves ; Chang, Cheng Yen ; Chen, Yun Ru ; Huang, Wen San ; Lee, Chi Ying. / Functional assessment of residues in the amino-and carboxyl-termini of crustacean hyperglycemic hormone (CHH) in the mud crab scylla olivacea using point-mutated peptides. In: PLoS ONE. 2015 ; Vol. 10, No. 8.
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abstract = "To assess functional importance of the residues in the amino-and carboxyl-termini of crustacean hyperglycemic hormone in the mud crab Scylla olivacea (Sco-CHH), both wild-type and point-mutated CHH peptides were produced with an amidated C-terminal end. Spectral analyses of circular dichroism, chromatographic retention time, and mass spectrometric analysis of the recombinant peptides indicate that they were close in conformation to native CHH and were produced with the intended substitutions. The recombinant peptides were subsequently used for an in vivo hyperglycemic assay. Two mutants (R13A and I69A rSco-CHH) completely lacked hyperglycemic activity, with temporal profiles similar to that of vehicle control. Temporal profiles of hyperglycemic responses elicited by 4 mutants (I2A, F3A, D12A, and D60A Sco-CHH) were different from that elicited by wild-type Sco-CHH; I2A was unique in that it exhibited significantly higher hyperglycemic activity, whereas the remaining 3 mutants showed lower activity. Four mutants (D4A, Q51A, E54A, and V72A rSco-CHH) elicited hyperglycemic responses with temporal profiles similar to those evoked by wild-type Sco-CHH. In contrast, the glycine-extended version of V72A rSco-CHH (V72A rSco-CHHGly) completely lost hyperglycemic activity. By comparing our study with previous ones of ion-transport peptide (ITP) and molt-inhibiting hormone (MIH) using deleted or point-mutated mutants, detail discussion is made regarding functionally important residues that are shared by both CHH and ITP (members of Group I of the CHH family), and those that discriminate CHH from ITP, and Group-I from Group-II peptides. Conclusions summarized in the present study provide insights into understanding of how functional diversification occurred within a peptide family of multifunctional members.",
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Functional assessment of residues in the amino-and carboxyl-termini of crustacean hyperglycemic hormone (CHH) in the mud crab scylla olivacea using point-mutated peptides. / Liu, Chun Jing; Huang, Shiau Shan; Toullec, Jean Yves; Chang, Cheng Yen; Chen, Yun Ru; Huang, Wen San; Lee, Chi Ying.

In: PLoS ONE, Vol. 10, No. 8, e0134983, 11.08.2015.

Research output: Contribution to journalArticle

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T1 - Functional assessment of residues in the amino-and carboxyl-termini of crustacean hyperglycemic hormone (CHH) in the mud crab scylla olivacea using point-mutated peptides

AU - Liu, Chun Jing

AU - Huang, Shiau Shan

AU - Toullec, Jean Yves

AU - Chang, Cheng Yen

AU - Chen, Yun Ru

AU - Huang, Wen San

AU - Lee, Chi Ying

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