Functional analysis of the domains in Cox11

Heather S. Carr, Andrew B. Maxfield, Yih Chern Horng, Dennis R. Winge

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Cox11 is an intrinsic mitochondrial membrane protein essential for the assembly of an active cytochrome c oxidase complex. Cox11 is tethered to the mitochondrial inner membrane by a single transmembrane helix. Domain mapping was carried out to determine the functional segments of the Cox11 protein. The C-terminal 189 residue Cu(I)-binding domain is shown to be exposed within the mitochondrial intermembrane space. This orientation was demonstrated by the proteolytic susceptibility of a C-terminal Myc epitope tag in mitoplasts but not intact mitochondria. Fusion of the N terminus of Cox11 to the matrix ribosomal protein Rsm22 results in a functional protein capable of suppressing the respiratory defect of both Δcox11 cells and Δrsm22 cells. The functionality of the fusion protein suggests that the Cox11 N terminus projects into the matrix. The fusion of the C-terminal segment of Cox11 to Rsm22 resembles a naturally occurring fusion of Cox11 in Schizosaccharomyces pombe to a sequence homologous to the Saccharomyces cerevisiae Rsm22. Studies on a series of SCO1/COX11 chimeras reveal that the matrix domain of Cox11 lacks a specific function, whereas the Cu(I) binding/donating function requires the yeast Cox11 sequence. The Cu(I)-binding domain from human Cox11 cannot functionally replace the yeast sequence. The copper domain of Cox11 may be an important docking motif for Cox1 or a Cox1-associated protein.

Original languageEnglish
Pages (from-to)22664-22669
Number of pages6
JournalJournal of Biological Chemistry
Volume280
Issue number24
DOIs
Publication statusPublished - 2005 Jun 17

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Functional analysis
Fusion reactions
Yeast
Mitochondrial Membranes
Proteins
Yeasts
Mitochondria
Schizosaccharomyces
Ribosomal Proteins
Mitochondrial Proteins
Electron Transport Complex IV
Sequence Homology
Saccharomyces cerevisiae
Copper
Epitopes
Membrane Proteins
Membranes
Defects

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Carr, Heather S. ; Maxfield, Andrew B. ; Horng, Yih Chern ; Winge, Dennis R. / Functional analysis of the domains in Cox11. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 24. pp. 22664-22669.
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Carr, HS, Maxfield, AB, Horng, YC & Winge, DR 2005, 'Functional analysis of the domains in Cox11', Journal of Biological Chemistry, vol. 280, no. 24, pp. 22664-22669. https://doi.org/10.1074/jbc.M414077200

Functional analysis of the domains in Cox11. / Carr, Heather S.; Maxfield, Andrew B.; Horng, Yih Chern; Winge, Dennis R.

In: Journal of Biological Chemistry, Vol. 280, No. 24, 17.06.2005, p. 22664-22669.

Research output: Contribution to journalArticle

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