Fluorescent detection of uric acid in biological samples through the inhibition of cobalt(II) catalyzed Amplex UltraRed

Chieh Yi Wang, Chang Wei Huang, Tzu Ting Wei, Mei Yao Wu, Yang-Wei Lin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A novel fluorescent probe consisting of cobalt(II)/hydrogen peroxide–Amplex®UltraRed (Co2+/H2O2–AUR) for determining uric acid (UA) concentration is reported. In the presence of H2O2, Co2+ions underwent oxidation to produce Co3+ions and hydroxyl radicals, which then reacted with the fluorescence reagent, AUR, forming a fluorescence product at a pH of 9.0. If UA was present, it underwent oxidation to form urea, parabanic acid, and allantoin. This inhibited the Co2+ions-catalyzed oxidation of AUR and resulted in a substantial decreasing in fluorescence intensity that was dependent on UA concentration. The buffer systems, pH values, and the concentrations of Co2+ions, AUR, and H2O2were crucial parameters in determining the sensitivity and selectivity of the method for sensing UA. Under optimal conditions, the fluorescence intensity decreased linearly with UA concentration in the 0.05–1.0 μmol L−1range (R2 = 0.94) with a detection limit of 20 nmol L−1at a signal-to-noise ratio of 3. The analytical recoveries in urine and human serum samples were 90%–100.7% and 90%–95% respectively. This method is simpler and more cost effective than are other optical methods for the detection of UA in biological samples.

Original languageEnglish
Pages (from-to)357-364
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume244
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

uric acid
Cobalt
Uric Acid
cobalt
Acids
Fluorescence
fluorescence
Ions
Oxidation
oxidation
ions
Allantoin
urine
hydroxyl radicals
Fluorescent Dyes
ureas
Hydroxyl Radical
serums
reagents
Urea

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

@article{72532624760f47f797be2b579acf4aa3,
title = "Fluorescent detection of uric acid in biological samples through the inhibition of cobalt(II) catalyzed Amplex UltraRed",
abstract = "A novel fluorescent probe consisting of cobalt(II)/hydrogen peroxide–Amplex{\circledR}UltraRed (Co2+/H2O2–AUR) for determining uric acid (UA) concentration is reported. In the presence of H2O2, Co2+ions underwent oxidation to produce Co3+ions and hydroxyl radicals, which then reacted with the fluorescence reagent, AUR, forming a fluorescence product at a pH of 9.0. If UA was present, it underwent oxidation to form urea, parabanic acid, and allantoin. This inhibited the Co2+ions-catalyzed oxidation of AUR and resulted in a substantial decreasing in fluorescence intensity that was dependent on UA concentration. The buffer systems, pH values, and the concentrations of Co2+ions, AUR, and H2O2were crucial parameters in determining the sensitivity and selectivity of the method for sensing UA. Under optimal conditions, the fluorescence intensity decreased linearly with UA concentration in the 0.05–1.0 μmol L−1range (R2 = 0.94) with a detection limit of 20 nmol L−1at a signal-to-noise ratio of 3. The analytical recoveries in urine and human serum samples were 90{\%}–100.7{\%} and 90{\%}–95{\%} respectively. This method is simpler and more cost effective than are other optical methods for the detection of UA in biological samples.",
author = "Wang, {Chieh Yi} and Huang, {Chang Wei} and Wei, {Tzu Ting} and Wu, {Mei Yao} and Yang-Wei Lin",
year = "2017",
month = "1",
day = "1",
doi = "10.1016/j.snb.2017.01.007",
language = "English",
volume = "244",
pages = "357--364",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

Fluorescent detection of uric acid in biological samples through the inhibition of cobalt(II) catalyzed Amplex UltraRed. / Wang, Chieh Yi; Huang, Chang Wei; Wei, Tzu Ting; Wu, Mei Yao; Lin, Yang-Wei.

In: Sensors and Actuators, B: Chemical, Vol. 244, 01.01.2017, p. 357-364.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fluorescent detection of uric acid in biological samples through the inhibition of cobalt(II) catalyzed Amplex UltraRed

AU - Wang, Chieh Yi

AU - Huang, Chang Wei

AU - Wei, Tzu Ting

AU - Wu, Mei Yao

AU - Lin, Yang-Wei

PY - 2017/1/1

Y1 - 2017/1/1

N2 - A novel fluorescent probe consisting of cobalt(II)/hydrogen peroxide–Amplex®UltraRed (Co2+/H2O2–AUR) for determining uric acid (UA) concentration is reported. In the presence of H2O2, Co2+ions underwent oxidation to produce Co3+ions and hydroxyl radicals, which then reacted with the fluorescence reagent, AUR, forming a fluorescence product at a pH of 9.0. If UA was present, it underwent oxidation to form urea, parabanic acid, and allantoin. This inhibited the Co2+ions-catalyzed oxidation of AUR and resulted in a substantial decreasing in fluorescence intensity that was dependent on UA concentration. The buffer systems, pH values, and the concentrations of Co2+ions, AUR, and H2O2were crucial parameters in determining the sensitivity and selectivity of the method for sensing UA. Under optimal conditions, the fluorescence intensity decreased linearly with UA concentration in the 0.05–1.0 μmol L−1range (R2 = 0.94) with a detection limit of 20 nmol L−1at a signal-to-noise ratio of 3. The analytical recoveries in urine and human serum samples were 90%–100.7% and 90%–95% respectively. This method is simpler and more cost effective than are other optical methods for the detection of UA in biological samples.

AB - A novel fluorescent probe consisting of cobalt(II)/hydrogen peroxide–Amplex®UltraRed (Co2+/H2O2–AUR) for determining uric acid (UA) concentration is reported. In the presence of H2O2, Co2+ions underwent oxidation to produce Co3+ions and hydroxyl radicals, which then reacted with the fluorescence reagent, AUR, forming a fluorescence product at a pH of 9.0. If UA was present, it underwent oxidation to form urea, parabanic acid, and allantoin. This inhibited the Co2+ions-catalyzed oxidation of AUR and resulted in a substantial decreasing in fluorescence intensity that was dependent on UA concentration. The buffer systems, pH values, and the concentrations of Co2+ions, AUR, and H2O2were crucial parameters in determining the sensitivity and selectivity of the method for sensing UA. Under optimal conditions, the fluorescence intensity decreased linearly with UA concentration in the 0.05–1.0 μmol L−1range (R2 = 0.94) with a detection limit of 20 nmol L−1at a signal-to-noise ratio of 3. The analytical recoveries in urine and human serum samples were 90%–100.7% and 90%–95% respectively. This method is simpler and more cost effective than are other optical methods for the detection of UA in biological samples.

UR - http://www.scopus.com/inward/record.url?scp=85008352826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008352826&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2017.01.007

DO - 10.1016/j.snb.2017.01.007

M3 - Article

AN - SCOPUS:85008352826

VL - 244

SP - 357

EP - 364

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -