Abstract
A facile, cost-effective, and sensitive fluorescent method for Pb 2+ ion detection had been developed using catechin synthesized gold nanoparticles (C-Au NPs). The Pb-catechin complexes and Pb-Au alloys that formed on the C-Au NPs surfaces allowed NPs to exhibit peroxidase-mimicking catalytic activity in the H2O2-mediated oxidation of Amplex UltraRed (AUR). In 5 mM Tris-acetate buffers at pH 7.0, the H2O 2-AUR-C-Au NP probe was highly selective (>100-fold) for Pb 2+ ions in the presence of other tested metal ions (K+, Ag+, Na+, Cd2+, Ni2+, Ca 2+, Hg2+, Sr2+, Co2+, Cu 2+, Ba2+, Fe2+, Mg2+, Cr 3+, and Fe3+ ions). The fluorescence intensity (excitation/emission maxima ∼540/588 nm) of the AUR product was proportional to the concentration of Pb2+ ions in the range of 10 nM-1.0 μM with a linear correlation (R2 = 0.99). The H2O 2-AUR-C-Au NP probe detected Pb2+ ions with a limit of detection (signal-to-noise ratio: 3) of 1.5 nM. The practicality of the H 2O2-AUR-C-Au NP probe was validated for the determination of Pb2+ ion concentration in environmental water and urine samples, demonstrating its advantages of simplicity, selectivity, and sensitivity.
| Original language | English |
|---|---|
| Pages (from-to) | 1503-1509 |
| Number of pages | 7 |
| Journal | ACS Applied Materials and Interfaces |
| Volume | 5 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2013 Mar 11 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Materials Science(all)
Cite this
}
Fluorescent detection of lead in environmental water and urine samples using enzyme mimics of catechin-synthesized Au nanoparticles. / Wu, Yan Shiuan; Huang, Fan Feng; Lin, Yang Wei.
In: ACS Applied Materials and Interfaces, Vol. 5, No. 4, 11.03.2013, p. 1503-1509.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fluorescent detection of lead in environmental water and urine samples using enzyme mimics of catechin-synthesized Au nanoparticles
AU - Wu, Yan Shiuan
AU - Huang, Fan Feng
AU - Lin, Yang Wei
PY - 2013/3/11
Y1 - 2013/3/11
N2 - A facile, cost-effective, and sensitive fluorescent method for Pb 2+ ion detection had been developed using catechin synthesized gold nanoparticles (C-Au NPs). The Pb-catechin complexes and Pb-Au alloys that formed on the C-Au NPs surfaces allowed NPs to exhibit peroxidase-mimicking catalytic activity in the H2O2-mediated oxidation of Amplex UltraRed (AUR). In 5 mM Tris-acetate buffers at pH 7.0, the H2O 2-AUR-C-Au NP probe was highly selective (>100-fold) for Pb 2+ ions in the presence of other tested metal ions (K+, Ag+, Na+, Cd2+, Ni2+, Ca 2+, Hg2+, Sr2+, Co2+, Cu 2+, Ba2+, Fe2+, Mg2+, Cr 3+, and Fe3+ ions). The fluorescence intensity (excitation/emission maxima ∼540/588 nm) of the AUR product was proportional to the concentration of Pb2+ ions in the range of 10 nM-1.0 μM with a linear correlation (R2 = 0.99). The H2O 2-AUR-C-Au NP probe detected Pb2+ ions with a limit of detection (signal-to-noise ratio: 3) of 1.5 nM. The practicality of the H 2O2-AUR-C-Au NP probe was validated for the determination of Pb2+ ion concentration in environmental water and urine samples, demonstrating its advantages of simplicity, selectivity, and sensitivity.
AB - A facile, cost-effective, and sensitive fluorescent method for Pb 2+ ion detection had been developed using catechin synthesized gold nanoparticles (C-Au NPs). The Pb-catechin complexes and Pb-Au alloys that formed on the C-Au NPs surfaces allowed NPs to exhibit peroxidase-mimicking catalytic activity in the H2O2-mediated oxidation of Amplex UltraRed (AUR). In 5 mM Tris-acetate buffers at pH 7.0, the H2O 2-AUR-C-Au NP probe was highly selective (>100-fold) for Pb 2+ ions in the presence of other tested metal ions (K+, Ag+, Na+, Cd2+, Ni2+, Ca 2+, Hg2+, Sr2+, Co2+, Cu 2+, Ba2+, Fe2+, Mg2+, Cr 3+, and Fe3+ ions). The fluorescence intensity (excitation/emission maxima ∼540/588 nm) of the AUR product was proportional to the concentration of Pb2+ ions in the range of 10 nM-1.0 μM with a linear correlation (R2 = 0.99). The H2O 2-AUR-C-Au NP probe detected Pb2+ ions with a limit of detection (signal-to-noise ratio: 3) of 1.5 nM. The practicality of the H 2O2-AUR-C-Au NP probe was validated for the determination of Pb2+ ion concentration in environmental water and urine samples, demonstrating its advantages of simplicity, selectivity, and sensitivity.
UR - http://www.scopus.com/inward/record.url?scp=84874583790&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874583790&partnerID=8YFLogxK
U2 - 10.1021/am3030454
DO - 10.1021/am3030454
M3 - Article
C2 - 23369297
AN - SCOPUS:84874583790
VL - 5
SP - 1503
EP - 1509
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
SN - 1944-8244
IS - 4
ER -