Fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates

Yang-Wei Lin, Chi Wei Liu, Huan Tsung Chang

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Abstract

We have developed a fluorescence technique for the detection of Hg 2+ and Pb2+ ions using polythymine (T33)/ benzothiazolium-4-quinolinium dimer derivative (TOTO-3) and polyguanine (G 33)/terbium ions (Tb3+) conjugates, respectively. Hg 2+ ions induce T33 to form folded structures, leading to increased fluorescence of the T33/TOTO-3 conjugates. Because Pb 2+ ions compete with Tb3+ ions to form complexes with G33, the extent of formation of the G33-Tb3+ complexes decreases upon increasing the Pb2+ concentration, leading to decreased fluorescence at 545 nm when excited at 290 nm. To minimize interference from Hg2+ ions during the detection of Pb2+ ions, we conducted two-step fluorescence measurements; prior to addition of the G33/Tb3+ probe, we recorded the fluorescence of a mixture of the T33/TOTO-3 conjugates and Hg2+ ions. The fluorescence signal obtained was linear with respect to the Hg2+ concentration over the range 25.0-500 nM (R2 = 0.99); for Pb 2+ ions, it was linear over the range 3.0-50 nM (R2 = 0.98). The limits of detection (at a signal-to-noise ratio of 3) for Hg 2+ and Pb2+ ions were 10.0 and 1.0 nM, respectively. Relative to other techniques for the detection of Hg2+ and Pb 2+ ions in soil and water samples, our present approach is simpler, faster, and more cost-effective.

Original languageEnglish
Pages (from-to)324-329
Number of pages6
JournalTalanta
Volume84
Issue number2
DOIs
Publication statusPublished - 2011 Apr 15

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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