Fluorescence detection of lead(II) ions through their induced catalytic activity of DNAzymes

Chi Lin Li, Kung Tien Liu, Yang Wei Lin, Huan Tsung Chang

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

We have developed a fluorescence approach for the highly selective and sensitive detection of Pb2+ ions using AGRO100, a G-quadruplex DNAzyme. The sensing strategy is based on Pb2+ ions inducing increased DNAzyme activity of AGRO100 in the presence of hemin, which acts as a cofactor to catalyze H2O2-mediated oxidation of Amplex UltraRed (AUR). A test of eight aptamers of various sequences for the detection of Pb2+ ions revealed that AGRO100 performed the best in terms of sensitivity. The AGRO100-AUR probe exhibited high selectivity (>100-fold) toward Pb2+ ions over other tested metal ions. The fluorescence intensity (excitation/emission maxima, ca. 561/592 nm) of the AUR product was proportional to the concentration of Pb2+ ions over the range 0-1000 nM, with a linear correlation (R2 = 0.98). For 5 mM Tris-acetate (pH 7.4) solutions in the presence and absence of 100 mM NaCl, the AGRO100-AUR probe provided limits of detection (signal-to-noise ratio = 3) for Pb2+ ions of 1.0 and 0.4 nM, respectively. We validated the practicality of the use of the AGRO100-AUR probe for the determination of the concentrations of Pb 2+ ions in soil samples. This approach allows the determination of the concentrations of Pb2+ ions with simplicity, selectivity, and sensitivity.

Original languageEnglish
Pages (from-to)225-230
Number of pages6
JournalAnalytical Chemistry
Volume83
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

Fingerprint

Catalytic DNA
Catalyst activity
Fluorescence
Ions
Hemin
Lead
Metal ions
AGRO 100
Signal to noise ratio
Acetates
Soils
Oxidation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Li, Chi Lin ; Liu, Kung Tien ; Lin, Yang Wei ; Chang, Huan Tsung. / Fluorescence detection of lead(II) ions through their induced catalytic activity of DNAzymes. In: Analytical Chemistry. 2011 ; Vol. 83, No. 1. pp. 225-230.
@article{27f033ec8a124d55913811c4f32d0e89,
title = "Fluorescence detection of lead(II) ions through their induced catalytic activity of DNAzymes",
abstract = "We have developed a fluorescence approach for the highly selective and sensitive detection of Pb2+ ions using AGRO100, a G-quadruplex DNAzyme. The sensing strategy is based on Pb2+ ions inducing increased DNAzyme activity of AGRO100 in the presence of hemin, which acts as a cofactor to catalyze H2O2-mediated oxidation of Amplex UltraRed (AUR). A test of eight aptamers of various sequences for the detection of Pb2+ ions revealed that AGRO100 performed the best in terms of sensitivity. The AGRO100-AUR probe exhibited high selectivity (>100-fold) toward Pb2+ ions over other tested metal ions. The fluorescence intensity (excitation/emission maxima, ca. 561/592 nm) of the AUR product was proportional to the concentration of Pb2+ ions over the range 0-1000 nM, with a linear correlation (R2 = 0.98). For 5 mM Tris-acetate (pH 7.4) solutions in the presence and absence of 100 mM NaCl, the AGRO100-AUR probe provided limits of detection (signal-to-noise ratio = 3) for Pb2+ ions of 1.0 and 0.4 nM, respectively. We validated the practicality of the use of the AGRO100-AUR probe for the determination of the concentrations of Pb 2+ ions in soil samples. This approach allows the determination of the concentrations of Pb2+ ions with simplicity, selectivity, and sensitivity.",
author = "Li, {Chi Lin} and Liu, {Kung Tien} and Lin, {Yang Wei} and Chang, {Huan Tsung}",
year = "2011",
month = "1",
day = "1",
doi = "10.1021/ac1028787",
language = "English",
volume = "83",
pages = "225--230",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "1",

}

Fluorescence detection of lead(II) ions through their induced catalytic activity of DNAzymes. / Li, Chi Lin; Liu, Kung Tien; Lin, Yang Wei; Chang, Huan Tsung.

In: Analytical Chemistry, Vol. 83, No. 1, 01.01.2011, p. 225-230.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fluorescence detection of lead(II) ions through their induced catalytic activity of DNAzymes

AU - Li, Chi Lin

AU - Liu, Kung Tien

AU - Lin, Yang Wei

AU - Chang, Huan Tsung

PY - 2011/1/1

Y1 - 2011/1/1

N2 - We have developed a fluorescence approach for the highly selective and sensitive detection of Pb2+ ions using AGRO100, a G-quadruplex DNAzyme. The sensing strategy is based on Pb2+ ions inducing increased DNAzyme activity of AGRO100 in the presence of hemin, which acts as a cofactor to catalyze H2O2-mediated oxidation of Amplex UltraRed (AUR). A test of eight aptamers of various sequences for the detection of Pb2+ ions revealed that AGRO100 performed the best in terms of sensitivity. The AGRO100-AUR probe exhibited high selectivity (>100-fold) toward Pb2+ ions over other tested metal ions. The fluorescence intensity (excitation/emission maxima, ca. 561/592 nm) of the AUR product was proportional to the concentration of Pb2+ ions over the range 0-1000 nM, with a linear correlation (R2 = 0.98). For 5 mM Tris-acetate (pH 7.4) solutions in the presence and absence of 100 mM NaCl, the AGRO100-AUR probe provided limits of detection (signal-to-noise ratio = 3) for Pb2+ ions of 1.0 and 0.4 nM, respectively. We validated the practicality of the use of the AGRO100-AUR probe for the determination of the concentrations of Pb 2+ ions in soil samples. This approach allows the determination of the concentrations of Pb2+ ions with simplicity, selectivity, and sensitivity.

AB - We have developed a fluorescence approach for the highly selective and sensitive detection of Pb2+ ions using AGRO100, a G-quadruplex DNAzyme. The sensing strategy is based on Pb2+ ions inducing increased DNAzyme activity of AGRO100 in the presence of hemin, which acts as a cofactor to catalyze H2O2-mediated oxidation of Amplex UltraRed (AUR). A test of eight aptamers of various sequences for the detection of Pb2+ ions revealed that AGRO100 performed the best in terms of sensitivity. The AGRO100-AUR probe exhibited high selectivity (>100-fold) toward Pb2+ ions over other tested metal ions. The fluorescence intensity (excitation/emission maxima, ca. 561/592 nm) of the AUR product was proportional to the concentration of Pb2+ ions over the range 0-1000 nM, with a linear correlation (R2 = 0.98). For 5 mM Tris-acetate (pH 7.4) solutions in the presence and absence of 100 mM NaCl, the AGRO100-AUR probe provided limits of detection (signal-to-noise ratio = 3) for Pb2+ ions of 1.0 and 0.4 nM, respectively. We validated the practicality of the use of the AGRO100-AUR probe for the determination of the concentrations of Pb 2+ ions in soil samples. This approach allows the determination of the concentrations of Pb2+ ions with simplicity, selectivity, and sensitivity.

UR - http://www.scopus.com/inward/record.url?scp=78650767968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650767968&partnerID=8YFLogxK

U2 - 10.1021/ac1028787

DO - 10.1021/ac1028787

M3 - Article

C2 - 21141923

AN - SCOPUS:78650767968

VL - 83

SP - 225

EP - 230

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 1

ER -