TY - GEN
T1 - Fiber-optic two-photon fluorescence correlation spectroscopy for remote cell flow velocity measurements
AU - Chang, Yu Chung
AU - Thomas, Thommey P.
AU - Baker, James R.
AU - Norris, Theodore B.
AU - Ye, Jing Yong
PY - 2010/12/1
Y1 - 2010/12/1
N2 - We report the use of a sensitive double-clad fiber (DCF) probe for remote cell flow velocity measurement by the means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. A flow velocity of as high as 40 cm/s has been measured. The theoretical lower limit is the self-diffusion of the cell, which is almost zero compared to typical body fluid flow. Owing to the unique feature of two-photon excitation, we can monitor multiple fluorescent markers simultaneously. Therefore, using high brightness nanoparticles to generate the reference signal, we can calibrate the real time flow velocity or even calculate the average size of the cells under measurement. The ability to conduct in vivo flow velocity measurement with single cell resolution using the fiber probe would provide a unique way for disease diagnosis or surveillance after treatment.
AB - We report the use of a sensitive double-clad fiber (DCF) probe for remote cell flow velocity measurement by the means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. A flow velocity of as high as 40 cm/s has been measured. The theoretical lower limit is the self-diffusion of the cell, which is almost zero compared to typical body fluid flow. Owing to the unique feature of two-photon excitation, we can monitor multiple fluorescent markers simultaneously. Therefore, using high brightness nanoparticles to generate the reference signal, we can calibrate the real time flow velocity or even calculate the average size of the cells under measurement. The ability to conduct in vivo flow velocity measurement with single cell resolution using the fiber probe would provide a unique way for disease diagnosis or surveillance after treatment.
UR - http://www.scopus.com/inward/record.url?scp=79951887439&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951887439&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2010.5689934
DO - 10.1109/ICSENS.2010.5689934
M3 - Conference contribution
AN - SCOPUS:79951887439
SN - 9781424481682
T3 - Proceedings of IEEE Sensors
SP - 1671
EP - 1674
BT - IEEE Sensors 2010 Conference, SENSORS 2010
T2 - 9th IEEE Sensors Conference 2010, SENSORS 2010
Y2 - 1 November 2010 through 4 November 2010
ER -