Fiber-optic two-photon fluorescence correlation spectroscopy for remote cell flow velocity measurements

Yu Chung Chang; Thommey P. Thomas; James R. Baker; Theodore B. Norris; Jing Yong Ye

doi:10.1109/ICSENS.2010.5689934

Fiber-optic two-photon fluorescence correlation spectroscopy for remote cell flow velocity measurements

Yu Chung Chang, Thommey P. Thomas, James R. Baker, Theodore B. Norris, Jing Yong Ye

Department of Electrical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report the use of a sensitive double-clad fiber (DCF) probe for remote cell flow velocity measurement by the means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. A flow velocity of as high as 40 cm/s has been measured. The theoretical lower limit is the self-diffusion of the cell, which is almost zero compared to typical body fluid flow. Owing to the unique feature of two-photon excitation, we can monitor multiple fluorescent markers simultaneously. Therefore, using high brightness nanoparticles to generate the reference signal, we can calibrate the real time flow velocity or even calculate the average size of the cells under measurement. The ability to conduct in vivo flow velocity measurement with single cell resolution using the fiber probe would provide a unique way for disease diagnosis or surveillance after treatment.

Original language	English
Title of host publication	IEEE Sensors 2010 Conference, SENSORS 2010
Pages	1671-1674
Number of pages	4
DOIs	https://doi.org/10.1109/ICSENS.2010.5689934
Publication status	Published - 2010 Dec 1
Event	9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States Duration: 2010 Nov 1 → 2010 Nov 4

Publication series

Name	Proceedings of IEEE Sensors

Other

Other	9th IEEE Sensors Conference 2010, SENSORS 2010
Country	United States
City	Waikoloa, HI
Period	10-11-01 → 10-11-04

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering

Access to Document

10.1109/ICSENS.2010.5689934

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Chang, Y. C., Thomas, T. P., Baker, J. R., Norris, T. B., & Ye, J. Y. (2010). Fiber-optic two-photon fluorescence correlation spectroscopy for remote cell flow velocity measurements. In IEEE Sensors 2010 Conference, SENSORS 2010 (pp. 1671-1674). [5689934] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2010.5689934

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title = "Fiber-optic two-photon fluorescence correlation spectroscopy for remote cell flow velocity measurements",

abstract = "We report the use of a sensitive double-clad fiber (DCF) probe for remote cell flow velocity measurement by the means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. A flow velocity of as high as 40 cm/s has been measured. The theoretical lower limit is the self-diffusion of the cell, which is almost zero compared to typical body fluid flow. Owing to the unique feature of two-photon excitation, we can monitor multiple fluorescent markers simultaneously. Therefore, using high brightness nanoparticles to generate the reference signal, we can calibrate the real time flow velocity or even calculate the average size of the cells under measurement. The ability to conduct in vivo flow velocity measurement with single cell resolution using the fiber probe would provide a unique way for disease diagnosis or surveillance after treatment.",

author = "Chang, {Yu Chung} and Thomas, {Thommey P.} and Baker, {James R.} and Norris, {Theodore B.} and Ye, {Jing Yong}",

year = "2010",

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doi = "10.1109/ICSENS.2010.5689934",

language = "English",

isbn = "9781424481682",

series = "Proceedings of IEEE Sensors",

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Chang, YC, Thomas, TP, Baker, JR, Norris, TB & Ye, JY 2010, Fiber-optic two-photon fluorescence correlation spectroscopy for remote cell flow velocity measurements. in IEEE Sensors 2010 Conference, SENSORS 2010., 5689934, Proceedings of IEEE Sensors, pp. 1671-1674, 9th IEEE Sensors Conference 2010, SENSORS 2010, Waikoloa, HI, United States, 10-11-01. https://doi.org/10.1109/ICSENS.2010.5689934

Fiber-optic two-photon fluorescence correlation spectroscopy for remote cell flow velocity measurements. / Chang, Yu Chung; Thomas, Thommey P.; Baker, James R.; Norris, Theodore B.; Ye, Jing Yong.

IEEE Sensors 2010 Conference, SENSORS 2010. 2010. p. 1671-1674 5689934 (Proceedings of IEEE Sensors).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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N2 - We report the use of a sensitive double-clad fiber (DCF) probe for remote cell flow velocity measurement by the means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. A flow velocity of as high as 40 cm/s has been measured. The theoretical lower limit is the self-diffusion of the cell, which is almost zero compared to typical body fluid flow. Owing to the unique feature of two-photon excitation, we can monitor multiple fluorescent markers simultaneously. Therefore, using high brightness nanoparticles to generate the reference signal, we can calibrate the real time flow velocity or even calculate the average size of the cells under measurement. The ability to conduct in vivo flow velocity measurement with single cell resolution using the fiber probe would provide a unique way for disease diagnosis or surveillance after treatment.

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