Abstract
We report the use of a sensitive double-clad fiber (DCF) probe for remote cell flow velocity measurement by the means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. A flow velocity of as high as 40 cm/s has been measured. The theoretical lower limit is the self-diffusion of the cell, which is almost zero compared to typical body fluid flow. Owing to the unique feature of two-photon excitation, we can monitor multiple fluorescent markers simultaneously. Therefore, using high brightness nanoparticles to generate the reference signal, we can calibrate the real time flow velocity or even calculate the average size of the cells under measurement. The ability to conduct in vivo flow velocity measurement with single cell resolution using the fiber probe would provide a unique way for disease diagnosis or surveillance after treatment.
| Original language | English |
|---|---|
| Title of host publication | IEEE Sensors 2010 Conference, SENSORS 2010 |
| Pages | 1671-1674 |
| Number of pages | 4 |
| DOIs | |
| Publication status | Published - 2010 Dec 1 |
| Event | 9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States Duration: 2010 Nov 1 → 2010 Nov 4 |
Publication series
| Name | Proceedings of IEEE Sensors |
|---|
Other
| Other | 9th IEEE Sensors Conference 2010, SENSORS 2010 |
|---|---|
| Country | United States |
| City | Waikoloa, HI |
| Period | 10-11-01 → 10-11-04 |
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All Science Journal Classification (ASJC) codes
- Electrical and Electronic Engineering
Cite this
}
Fiber-optic two-photon fluorescence correlation spectroscopy for remote cell flow velocity measurements. / Chang, Yu Chung; Thomas, Thommey P.; Baker, James R.; Norris, Theodore B.; Ye, Jing Yong.
IEEE Sensors 2010 Conference, SENSORS 2010. 2010. p. 1671-1674 5689934 (Proceedings of IEEE Sensors).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Fiber-optic two-photon fluorescence correlation spectroscopy for remote cell flow velocity measurements
AU - Chang, Yu Chung
AU - Thomas, Thommey P.
AU - Baker, James R.
AU - Norris, Theodore B.
AU - Ye, Jing Yong
PY - 2010/12/1
Y1 - 2010/12/1
N2 - We report the use of a sensitive double-clad fiber (DCF) probe for remote cell flow velocity measurement by the means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. A flow velocity of as high as 40 cm/s has been measured. The theoretical lower limit is the self-diffusion of the cell, which is almost zero compared to typical body fluid flow. Owing to the unique feature of two-photon excitation, we can monitor multiple fluorescent markers simultaneously. Therefore, using high brightness nanoparticles to generate the reference signal, we can calibrate the real time flow velocity or even calculate the average size of the cells under measurement. The ability to conduct in vivo flow velocity measurement with single cell resolution using the fiber probe would provide a unique way for disease diagnosis or surveillance after treatment.
AB - We report the use of a sensitive double-clad fiber (DCF) probe for remote cell flow velocity measurement by the means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. A flow velocity of as high as 40 cm/s has been measured. The theoretical lower limit is the self-diffusion of the cell, which is almost zero compared to typical body fluid flow. Owing to the unique feature of two-photon excitation, we can monitor multiple fluorescent markers simultaneously. Therefore, using high brightness nanoparticles to generate the reference signal, we can calibrate the real time flow velocity or even calculate the average size of the cells under measurement. The ability to conduct in vivo flow velocity measurement with single cell resolution using the fiber probe would provide a unique way for disease diagnosis or surveillance after treatment.
UR - http://www.scopus.com/inward/record.url?scp=79951887439&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951887439&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2010.5689934
DO - 10.1109/ICSENS.2010.5689934
M3 - Conference contribution
AN - SCOPUS:79951887439
SN - 9781424481682
T3 - Proceedings of IEEE Sensors
SP - 1671
EP - 1674
BT - IEEE Sensors 2010 Conference, SENSORS 2010
ER -