Fiber-optic two-photon fluorescence correlation spectroscopy for cell flow measurements

Research output: Contribution to journalArticle

Abstract

Quantification of sampled blood volume is important in rare circulating cell detection. A sensitive double-clad fiber probe is utilized for in situ blood flow velocity measurements and cell size analysis by means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure flow velocities of fluorescently labeled cells in whole blood has been demonstrated. By simultaneously monitoring the distinct fluorescence signals from the labeled cells and fluorescent microspheres with known size, the flow velocity can be calibrated in real time and the average cell size can be calculated. The measured flow velocities agree with the theoretical estimation. However, the measured cell size is larger than the typical size, which is due to the comparable size between the cell and the probe area. Translational FCS measurements on particles of different sizes are conducted to confirm the particle size effect on the measured transit times.

Original languageEnglish
Article number6205329
Pages (from-to)2750-2755
Number of pages6
JournalIEEE Sensors Journal
Volume12
Issue number9
DOIs
Publication statusPublished - 2012 Aug 9

Fingerprint

flow measurement
Flow measurement
Flow velocity
Fiber optics
fiber optics
Photons
Fluorescence
Spectroscopy
fluorescence
Blood
photons
cells
spectroscopy
flow velocity
Microspheres
Velocity measurement
Particle size
blood volume
Fibers
Monitoring

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Instrumentation

Cite this

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abstract = "Quantification of sampled blood volume is important in rare circulating cell detection. A sensitive double-clad fiber probe is utilized for in situ blood flow velocity measurements and cell size analysis by means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure flow velocities of fluorescently labeled cells in whole blood has been demonstrated. By simultaneously monitoring the distinct fluorescence signals from the labeled cells and fluorescent microspheres with known size, the flow velocity can be calibrated in real time and the average cell size can be calculated. The measured flow velocities agree with the theoretical estimation. However, the measured cell size is larger than the typical size, which is due to the comparable size between the cell and the probe area. Translational FCS measurements on particles of different sizes are conducted to confirm the particle size effect on the measured transit times.",
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Fiber-optic two-photon fluorescence correlation spectroscopy for cell flow measurements. / Chang, Yu-Chung.

In: IEEE Sensors Journal, Vol. 12, No. 9, 6205329, 09.08.2012, p. 2750-2755.

Research output: Contribution to journalArticle

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