Fiber-optic two-photon fluorescence correlation spectroscopy for cell flow measurements

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Quantification of sampled blood volume is important in rare circulating cell detection. A sensitive double-clad fiber probe is utilized for in situ blood flow velocity measurements and cell size analysis by means of two-photon excited fluorescence correlation spectroscopy (FCS). The ability to measure flow velocities of fluorescently labeled cells in whole blood has been demonstrated. By simultaneously monitoring the distinct fluorescence signals from the labeled cells and fluorescent microspheres with known size, the flow velocity can be calibrated in real time and the average cell size can be calculated. The measured flow velocities agree with the theoretical estimation. However, the measured cell size is larger than the typical size, which is due to the comparable size between the cell and the probe area. Translational FCS measurements on particles of different sizes are conducted to confirm the particle size effect on the measured transit times.

Original languageEnglish
Article number6205329
Pages (from-to)2750-2755
Number of pages6
JournalIEEE Sensors Journal
Issue number9
Publication statusPublished - 2012 Aug 9

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering

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