Fiber-optic multiphoton in vivo flow cytometry

Yu-Chung Chang, Jing Yong Ye, Thommey Thomas, Zhengyi Cao, Alina Kotlyar, James R. Baker, Theodore B. Norris

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in a live mouse. High detection efficiency of GFP-expressing cells is demonstrated, and the initial dynamics of injected circulating cells is observed.

Original languageEnglish
Title of host publicationConference on Lasers and Electro-Optics, CLEO 2009
Publication statusPublished - 2009 Dec 1
EventConference on Lasers and Electro-Optics, CLEO 2009 - Baltimore, MD, United States
Duration: 2009 May 312009 Jun 5

Other

OtherConference on Lasers and Electro-Optics, CLEO 2009
CountryUnited States
CityBaltimore, MD
Period09-05-3109-06-05

Fingerprint

cytometry
Flow cytometry
Fiber optics
fiber optics
Photons
Fibers
cells
mice
fibers
probes
photons

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Chang, Y-C., Ye, J. Y., Thomas, T., Cao, Z., Kotlyar, A., Baker, J. R., & Norris, T. B. (2009). Fiber-optic multiphoton in vivo flow cytometry. In Conference on Lasers and Electro-Optics, CLEO 2009
Chang, Yu-Chung ; Ye, Jing Yong ; Thomas, Thommey ; Cao, Zhengyi ; Kotlyar, Alina ; Baker, James R. ; Norris, Theodore B. / Fiber-optic multiphoton in vivo flow cytometry. Conference on Lasers and Electro-Optics, CLEO 2009. 2009.
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Chang, Y-C, Ye, JY, Thomas, T, Cao, Z, Kotlyar, A, Baker, JR & Norris, TB 2009, Fiber-optic multiphoton in vivo flow cytometry. in Conference on Lasers and Electro-Optics, CLEO 2009. Conference on Lasers and Electro-Optics, CLEO 2009, Baltimore, MD, United States, 09-05-31.

Fiber-optic multiphoton in vivo flow cytometry. / Chang, Yu-Chung; Ye, Jing Yong; Thomas, Thommey; Cao, Zhengyi; Kotlyar, Alina; Baker, James R.; Norris, Theodore B.

Conference on Lasers and Electro-Optics, CLEO 2009. 2009.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Fiber-optic multiphoton in vivo flow cytometry

AU - Chang, Yu-Chung

AU - Ye, Jing Yong

AU - Thomas, Thommey

AU - Cao, Zhengyi

AU - Kotlyar, Alina

AU - Baker, James R.

AU - Norris, Theodore B.

PY - 2009/12/1

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N2 - We demonstrate the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in a live mouse. High detection efficiency of GFP-expressing cells is demonstrated, and the initial dynamics of injected circulating cells is observed.

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M3 - Conference contribution

SN - 9781557528698

BT - Conference on Lasers and Electro-Optics, CLEO 2009

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Chang Y-C, Ye JY, Thomas T, Cao Z, Kotlyar A, Baker JR et al. Fiber-optic multiphoton in vivo flow cytometry. In Conference on Lasers and Electro-Optics, CLEO 2009. 2009