Fiber-optic multiphoton flow cytometry in whole blood and in vivo

Yu Chung Chang, Jing Yong Ye, Thommey P. Thomas, Zhengyi Cao, Alina Kotlyar, Eric R. Tkaczyk, James R. Baker

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Circulating tumor cells in the bloodstream are sensitive indicators for metastasis and disease prognosis. Circulating cells have usually been monitored via extraction from blood, and more recently in vivo using freespace optics; however, long-term intravital monitoring of rare circulating cells remains a major challenge. We demonstrate the application of a two-photon-fluorescence optical fiber probe for the detection of cells in whole blood and in vivo. A double-clad fiber was used to enhance the detection sensitivity. Two-channel detection was employed to enable simultaneous measurement of multiple fluorescent markers. Because the fiber probe circumvents scattering and absorption from whole blood, the detected signal strength from fluorescent cells was found to be similar in phosphate-buffered saline (PBS) and in whole blood. The detection efficiency of cells labeled with the membrane-binding dye 1,1(-dioctadecyl-3,3,3',3'- tetramethylindoldicarbocyanine, 4- chlorobenzenesulfonate (DiD) was demonstrated to be the same in PBS and in whole blood. A high detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was also demonstrated. To characterize in vivo detection, DiDlabeled untransfected and GFP-transfected cells were injected into live mice, and the cell circulation dynamics was monitored in real time. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed ex vivo in whole blood.

Original languageEnglish
Article number047004
JournalJournal of Biomedical Optics
Volume15
Issue number4
DOIs
Publication statusPublished - 2010 Jul 1

Fingerprint

cytometry
Flow cytometry
Fiber optics
blood
fiber optics
Blood
cells
Green Fluorescent Proteins
Proteins
Phosphates
proteins
phosphates
Fibers
fibers
prognosis
probes
Optical fibers
Tumors
Optics
metastasis

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Chang, Y. C., Ye, J. Y., Thomas, T. P., Cao, Z., Kotlyar, A., Tkaczyk, E. R., & Baker, J. R. (2010). Fiber-optic multiphoton flow cytometry in whole blood and in vivo. Journal of Biomedical Optics, 15(4), [047004]. https://doi.org/10.1117/1.3463481
Chang, Yu Chung ; Ye, Jing Yong ; Thomas, Thommey P. ; Cao, Zhengyi ; Kotlyar, Alina ; Tkaczyk, Eric R. ; Baker, James R. / Fiber-optic multiphoton flow cytometry in whole blood and in vivo. In: Journal of Biomedical Optics. 2010 ; Vol. 15, No. 4.
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Chang, YC, Ye, JY, Thomas, TP, Cao, Z, Kotlyar, A, Tkaczyk, ER & Baker, JR 2010, 'Fiber-optic multiphoton flow cytometry in whole blood and in vivo', Journal of Biomedical Optics, vol. 15, no. 4, 047004. https://doi.org/10.1117/1.3463481

Fiber-optic multiphoton flow cytometry in whole blood and in vivo. / Chang, Yu Chung; Ye, Jing Yong; Thomas, Thommey P.; Cao, Zhengyi; Kotlyar, Alina; Tkaczyk, Eric R.; Baker, James R.

In: Journal of Biomedical Optics, Vol. 15, No. 4, 047004, 01.07.2010.

Research output: Contribution to journalArticle

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