Exocytosis of a single bovine adrenal chromaffin cell: The electrical and morphological studies

Chia Chang Tsai, Chih Cheng Yang, Po Yuan Shin, Cen-Shawn Wu, Chii Dong Chen, Chien Yuan Pan, Yit Tsong Chen

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Exocytosis of a single bovine adrenal chromaffin cell, triggered by histamine stimulation, was investigated via the electric responses detected with single-walled carbon-nanotube field-effect transistors (SWCNT-FET) and the morphological changes acquired by atomic force microscopy (AFM). Secretion of chromogranin A (CgA), stored in the vesicles of a single chromaffin cell, can be monitored in situ by the antibody against CgA (CgA-antibody) functionalized on the SWCNT-FET devices. The SWCNT-FET can further discriminate the amount of released CgA with different levels of histamine stimulations. The AFM morphological studies on a chromaffin cell indicate that the depression structures on the cell surface, caused by the histamine-evoked exocytotic fusion pores, appeared much more frequently than those without histamine stimulation or with the pretreatment of mepyramine before histamine stimulation. The vesicle diameters are about 50 nm calculated from the obtained three-dimensional AFM images. In comparison, the fusion pores of chromaffin cells stimulated by high-K+ buffer solution were also investigated to have a wider-ranging distribution of vesicle diameters of 60-260 nm. This work demonstrates that the combination of novel techniques, SWCNTFET and AFM, can provide further insights into the fundamental properties of exocytosis in neuroendocrine cells.

Original languageEnglish
Pages (from-to)9165-9173
Number of pages9
JournalJournal of Physical Chemistry B
Volume112
Issue number30
DOIs
Publication statusPublished - 2008 Jul 31

Fingerprint

histamines
Carbon nanotube field effect transistors
Chromogranin A
Histamine
Atomic force microscopy
Single-walled carbon nanotubes (SWCN)
stimulation
cells
Antibodies
atomic force microscopy
Fusion reactions
field effect transistors
carbon nanotubes
antibodies
Pyrilamine
fusion
porosity
secretions
Buffers
pretreatment

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Tsai, Chia Chang ; Yang, Chih Cheng ; Shin, Po Yuan ; Wu, Cen-Shawn ; Chen, Chii Dong ; Pan, Chien Yuan ; Chen, Yit Tsong. / Exocytosis of a single bovine adrenal chromaffin cell : The electrical and morphological studies. In: Journal of Physical Chemistry B. 2008 ; Vol. 112, No. 30. pp. 9165-9173.
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Exocytosis of a single bovine adrenal chromaffin cell : The electrical and morphological studies. / Tsai, Chia Chang; Yang, Chih Cheng; Shin, Po Yuan; Wu, Cen-Shawn; Chen, Chii Dong; Pan, Chien Yuan; Chen, Yit Tsong.

In: Journal of Physical Chemistry B, Vol. 112, No. 30, 31.07.2008, p. 9165-9173.

Research output: Contribution to journalArticle

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