Ethanol production from lignocelluloses by native strain Klebsiella oxytoca THLC0409

Dang Thuan Tran, Chi Wen Lin, Chi Yung Lai, Chih Hung Wu

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10 Citations (Scopus)


The native strain Klebsiella oxytoca THLC0409 was isolated from a lignocelluloses-degrading microflora. Preliminary investigations were conducted to obtain the optimal growth conditions of the strain. Batch operations were applied for producing ethanol from various lignocelluloses via direct microbial conversion (DMC). The strain THLC0409 was a naturally-occurring cellulolytic microorganism having high capability of utilizing celluloses (avicel, α-cellulose) and natural lignocelluloses (corncob, Napiergrass, purified bamboo, raw bamboo, rice straw). However, THLC0409 is an enteric bacterium lacking cellulase and xylanase activity. The ethanol productions varied depending on the compositions of substrate lignocelluloses. Under the optimal conditions, ethanol yields on corncob and Napiergrass reached 0.0623 and 0.0475 g g -1, respectively, which were far higher than those on avicel (0.019 g g -1), α-cellulose (0.02 g g -1), purified bamboo (0.02 g g -1), raw bamboo (0.018 g g -1), and rice straw (0.016 g g -1). Moreover, ethanol yields on oat-extracted xylan and corncob-extracted xylan were 0.15 and 0.04 g g -1, respectively.

Original languageEnglish
Pages (from-to)389-396
Number of pages8
JournalWaste and Biomass Valorization
Issue number4
Publication statusPublished - 2011 Nov

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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