Establishment of a trimodality analytical platform for tracing, imaging and quantification of gold nanoparticles in animals by radiotracer techniques

Chien Hung Chen, Fong Sian Lin, Wei Neng Liao, Sanching L. Liang, Min Hua Chen, Yo Wen Chen, Wan Yu Lin, Ming-Hua Hsu, Mei Ya Wang, Jinn Jer Peir, Fong In Chou, Ching Ya Chen, Sih Yu Chen, Su Chin Huang, Mo Hsiung Yang, Dueng Yuan Hueng, Yeukuang Hwu, Chung Shi Yang, Jen Kun Chen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study aims to establish a 198Au-radiotracer technique for in vivo tracing, rapid quantification, and ex vivo visualization of PEGylated gold nanoparticles (GNPs) in animals, organs and tissue dissections. The advantages of GNPs lie in its superior optical property, biocompatibility and versatile conjugation chemistry, which are promising to develop diagnostic probes and drug delivery systems. 198Au is used as a radiotracer because it simultaneously emits beta and gamma radiations with proper energy and half-life; therefore, 198Au can be used for bioanalytical purposes. The 198Au-tagged radioactive gold nanoparticles (198Au-GNPs) were prepared simply by irradiating the GNPs in a nuclear reactor through the 197Au(n,γ)198Au reaction and subsequently the 198Au-GNPs were subjected to surface modification with polyethylene glycol to form PEGylated 198Au-GNPs. The 198Au-GNPs retained physicochemical properties that were the same as those of GNP before neutron irradiation. Pharmacokinetic and biodisposition studies were performed by intravenously injecting three types of 198Au-GNPs with or without PEGylation into mice; the γ radiation in blood specimens and dissected organs was then measured. The 198Au-radiotracer technique enables rapid quantification freed from tedious sample preparation and shows more than 95% recovery of injected GNPs. Clinical gamma scintigraphy was proved feasible to explore spatial- and temporal-resolved biodisposition of 198Au-GNPs in living animals. Moreover, autoradiography, which recorded beta particles from 198Au, enabled visualizing the heterogeneous biodisposition of 198Au-GNPs in different microenvironments and tissues. In this study, the 198Au-radiotracer technique facilitated creating a trimodality analytical platform for tracing, quantifying and imaging GNPs in animals.

Original languageEnglish
Pages (from-to)601-608
Number of pages8
JournalAnalytical Chemistry
Volume87
Issue number1
DOIs
Publication statusPublished - 2015 Jan 6

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Gold
Animals
Nanoparticles
Imaging techniques
Tissue
Dissection
Pharmacokinetics
Neutron irradiation
Nuclear reactors
Biocompatibility
Gamma rays
Surface treatment
Blood
Visualization
Optical properties
Radiation
Recovery

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Chen, Chien Hung ; Lin, Fong Sian ; Liao, Wei Neng ; Liang, Sanching L. ; Chen, Min Hua ; Chen, Yo Wen ; Lin, Wan Yu ; Hsu, Ming-Hua ; Wang, Mei Ya ; Peir, Jinn Jer ; Chou, Fong In ; Chen, Ching Ya ; Chen, Sih Yu ; Huang, Su Chin ; Yang, Mo Hsiung ; Hueng, Dueng Yuan ; Hwu, Yeukuang ; Yang, Chung Shi ; Chen, Jen Kun. / Establishment of a trimodality analytical platform for tracing, imaging and quantification of gold nanoparticles in animals by radiotracer techniques. In: Analytical Chemistry. 2015 ; Vol. 87, No. 1. pp. 601-608.
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title = "Establishment of a trimodality analytical platform for tracing, imaging and quantification of gold nanoparticles in animals by radiotracer techniques",
abstract = "This study aims to establish a 198Au-radiotracer technique for in vivo tracing, rapid quantification, and ex vivo visualization of PEGylated gold nanoparticles (GNPs) in animals, organs and tissue dissections. The advantages of GNPs lie in its superior optical property, biocompatibility and versatile conjugation chemistry, which are promising to develop diagnostic probes and drug delivery systems. 198Au is used as a radiotracer because it simultaneously emits beta and gamma radiations with proper energy and half-life; therefore, 198Au can be used for bioanalytical purposes. The 198Au-tagged radioactive gold nanoparticles (198Au-GNPs) were prepared simply by irradiating the GNPs in a nuclear reactor through the 197Au(n,γ)198Au reaction and subsequently the 198Au-GNPs were subjected to surface modification with polyethylene glycol to form PEGylated 198Au-GNPs. The 198Au-GNPs retained physicochemical properties that were the same as those of GNP before neutron irradiation. Pharmacokinetic and biodisposition studies were performed by intravenously injecting three types of 198Au-GNPs with or without PEGylation into mice; the γ radiation in blood specimens and dissected organs was then measured. The 198Au-radiotracer technique enables rapid quantification freed from tedious sample preparation and shows more than 95{\%} recovery of injected GNPs. Clinical gamma scintigraphy was proved feasible to explore spatial- and temporal-resolved biodisposition of 198Au-GNPs in living animals. Moreover, autoradiography, which recorded beta particles from 198Au, enabled visualizing the heterogeneous biodisposition of 198Au-GNPs in different microenvironments and tissues. In this study, the 198Au-radiotracer technique facilitated creating a trimodality analytical platform for tracing, quantifying and imaging GNPs in animals.",
author = "Chen, {Chien Hung} and Lin, {Fong Sian} and Liao, {Wei Neng} and Liang, {Sanching L.} and Chen, {Min Hua} and Chen, {Yo Wen} and Lin, {Wan Yu} and Ming-Hua Hsu and Wang, {Mei Ya} and Peir, {Jinn Jer} and Chou, {Fong In} and Chen, {Ching Ya} and Chen, {Sih Yu} and Huang, {Su Chin} and Yang, {Mo Hsiung} and Hueng, {Dueng Yuan} and Yeukuang Hwu and Yang, {Chung Shi} and Chen, {Jen Kun}",
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Chen, CH, Lin, FS, Liao, WN, Liang, SL, Chen, MH, Chen, YW, Lin, WY, Hsu, M-H, Wang, MY, Peir, JJ, Chou, FI, Chen, CY, Chen, SY, Huang, SC, Yang, MH, Hueng, DY, Hwu, Y, Yang, CS & Chen, JK 2015, 'Establishment of a trimodality analytical platform for tracing, imaging and quantification of gold nanoparticles in animals by radiotracer techniques', Analytical Chemistry, vol. 87, no. 1, pp. 601-608. https://doi.org/10.1021/ac503260f

Establishment of a trimodality analytical platform for tracing, imaging and quantification of gold nanoparticles in animals by radiotracer techniques. / Chen, Chien Hung; Lin, Fong Sian; Liao, Wei Neng; Liang, Sanching L.; Chen, Min Hua; Chen, Yo Wen; Lin, Wan Yu; Hsu, Ming-Hua; Wang, Mei Ya; Peir, Jinn Jer; Chou, Fong In; Chen, Ching Ya; Chen, Sih Yu; Huang, Su Chin; Yang, Mo Hsiung; Hueng, Dueng Yuan; Hwu, Yeukuang; Yang, Chung Shi; Chen, Jen Kun.

In: Analytical Chemistry, Vol. 87, No. 1, 06.01.2015, p. 601-608.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Establishment of a trimodality analytical platform for tracing, imaging and quantification of gold nanoparticles in animals by radiotracer techniques

AU - Chen, Chien Hung

AU - Lin, Fong Sian

AU - Liao, Wei Neng

AU - Liang, Sanching L.

AU - Chen, Min Hua

AU - Chen, Yo Wen

AU - Lin, Wan Yu

AU - Hsu, Ming-Hua

AU - Wang, Mei Ya

AU - Peir, Jinn Jer

AU - Chou, Fong In

AU - Chen, Ching Ya

AU - Chen, Sih Yu

AU - Huang, Su Chin

AU - Yang, Mo Hsiung

AU - Hueng, Dueng Yuan

AU - Hwu, Yeukuang

AU - Yang, Chung Shi

AU - Chen, Jen Kun

PY - 2015/1/6

Y1 - 2015/1/6

N2 - This study aims to establish a 198Au-radiotracer technique for in vivo tracing, rapid quantification, and ex vivo visualization of PEGylated gold nanoparticles (GNPs) in animals, organs and tissue dissections. The advantages of GNPs lie in its superior optical property, biocompatibility and versatile conjugation chemistry, which are promising to develop diagnostic probes and drug delivery systems. 198Au is used as a radiotracer because it simultaneously emits beta and gamma radiations with proper energy and half-life; therefore, 198Au can be used for bioanalytical purposes. The 198Au-tagged radioactive gold nanoparticles (198Au-GNPs) were prepared simply by irradiating the GNPs in a nuclear reactor through the 197Au(n,γ)198Au reaction and subsequently the 198Au-GNPs were subjected to surface modification with polyethylene glycol to form PEGylated 198Au-GNPs. The 198Au-GNPs retained physicochemical properties that were the same as those of GNP before neutron irradiation. Pharmacokinetic and biodisposition studies were performed by intravenously injecting three types of 198Au-GNPs with or without PEGylation into mice; the γ radiation in blood specimens and dissected organs was then measured. The 198Au-radiotracer technique enables rapid quantification freed from tedious sample preparation and shows more than 95% recovery of injected GNPs. Clinical gamma scintigraphy was proved feasible to explore spatial- and temporal-resolved biodisposition of 198Au-GNPs in living animals. Moreover, autoradiography, which recorded beta particles from 198Au, enabled visualizing the heterogeneous biodisposition of 198Au-GNPs in different microenvironments and tissues. In this study, the 198Au-radiotracer technique facilitated creating a trimodality analytical platform for tracing, quantifying and imaging GNPs in animals.

AB - This study aims to establish a 198Au-radiotracer technique for in vivo tracing, rapid quantification, and ex vivo visualization of PEGylated gold nanoparticles (GNPs) in animals, organs and tissue dissections. The advantages of GNPs lie in its superior optical property, biocompatibility and versatile conjugation chemistry, which are promising to develop diagnostic probes and drug delivery systems. 198Au is used as a radiotracer because it simultaneously emits beta and gamma radiations with proper energy and half-life; therefore, 198Au can be used for bioanalytical purposes. The 198Au-tagged radioactive gold nanoparticles (198Au-GNPs) were prepared simply by irradiating the GNPs in a nuclear reactor through the 197Au(n,γ)198Au reaction and subsequently the 198Au-GNPs were subjected to surface modification with polyethylene glycol to form PEGylated 198Au-GNPs. The 198Au-GNPs retained physicochemical properties that were the same as those of GNP before neutron irradiation. Pharmacokinetic and biodisposition studies were performed by intravenously injecting three types of 198Au-GNPs with or without PEGylation into mice; the γ radiation in blood specimens and dissected organs was then measured. The 198Au-radiotracer technique enables rapid quantification freed from tedious sample preparation and shows more than 95% recovery of injected GNPs. Clinical gamma scintigraphy was proved feasible to explore spatial- and temporal-resolved biodisposition of 198Au-GNPs in living animals. Moreover, autoradiography, which recorded beta particles from 198Au, enabled visualizing the heterogeneous biodisposition of 198Au-GNPs in different microenvironments and tissues. In this study, the 198Au-radiotracer technique facilitated creating a trimodality analytical platform for tracing, quantifying and imaging GNPs in animals.

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