Effect of silymarin on curcumin-induced mortality in zebrafish (Danio rerio) embryos and larvae

Rong Jen Shiau, Pei Chun Shih, Yu Der Wen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In presence of 7.5 μM of curcumin, no embryos or larva of zebrafish survived 3 days of incubation; however, co-incubation with 144 μg/ml silymarin increased the survival rates of curcumin-treated embryos and larvae to about 70%. Moreover, in presence of 12.5 μM curcumin, all embryos died after 2 days of incubation; however, co-treatment with 144 μg/ml silymarin increased the survival rates of curcumin-treated embryos and larvae up to 60 and 50%, respectively. This protective effect was not found in the other phenolic compounds viz., ferulic acid, naringin, or crocin, tested. Finally, using a fluorescence microscope, accumulation of less curcumin has observed in the edema sac area of the larvae co-treated with curcumin and silymarin than in the larvae treated with curcumin only. The result suggests that the protective effects of silymarin may be due to a decreased accumulation of curcumin in the fish body.

Original languageEnglish
Pages (from-to)491-497
Number of pages7
JournalIndian Journal of Experimental Biology
Volume49
Issue number7
Publication statusPublished - 2011 Jul 1

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Silymarin
Curcumin
Zebrafish
Larva
Embryonic Structures
Mortality
ferulic acid
Survival Rate
Edema
Fishes
Fluorescence

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "In presence of 7.5 μM of curcumin, no embryos or larva of zebrafish survived 3 days of incubation; however, co-incubation with 144 μg/ml silymarin increased the survival rates of curcumin-treated embryos and larvae to about 70{\%}. Moreover, in presence of 12.5 μM curcumin, all embryos died after 2 days of incubation; however, co-treatment with 144 μg/ml silymarin increased the survival rates of curcumin-treated embryos and larvae up to 60 and 50{\%}, respectively. This protective effect was not found in the other phenolic compounds viz., ferulic acid, naringin, or crocin, tested. Finally, using a fluorescence microscope, accumulation of less curcumin has observed in the edema sac area of the larvae co-treated with curcumin and silymarin than in the larvae treated with curcumin only. The result suggests that the protective effects of silymarin may be due to a decreased accumulation of curcumin in the fish body.",
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Effect of silymarin on curcumin-induced mortality in zebrafish (Danio rerio) embryos and larvae. / Shiau, Rong Jen; Shih, Pei Chun; Wen, Yu Der.

In: Indian Journal of Experimental Biology, Vol. 49, No. 7, 01.07.2011, p. 491-497.

Research output: Contribution to journalArticle

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AU - Shiau, Rong Jen

AU - Shih, Pei Chun

AU - Wen, Yu Der

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