Effect of Ethanol Extracts of Three Chinese Medicinal Plants with Anti-diarrheal Properties on Ion Transport of the Rat Intestinal Epithelia

Jong-Chang Tsai, Shuli Tsai, Weng Cheng Chang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Effects of ethanol extracts of three Chinese medicinal plants, namely, Qinpi (Fraxini cortex), Kushen (Sophora flavescens, AITON), and Huanglian (Coptis teeta, WALLICH), on ion transport of the rat intestinal epithelia were determined in this study. Rat intestinal epithelia mounted in an Ussing chamber attached to a voltage/current clamp were used for measuring changes in the short circuit current across the epithelia. Activation of the intestinal epithelia by serosal administration of 5 μM forskolin resulted in an increase in basal short circuit current. The ethanol extracts of each of the three plants partially reduced the current stimulated by forskolin. In the following experiments, ouabain and bumetanide were added prior to adding the ethanol extract of these plants for revealing their effect on Na+ and Cl- movement. The results suggest that the ethanol extract of the Qinpi would affect Cl- transport. On the contrary, the ethanol extract of Kushen would affect Na+ transport rather than Cl - movement. This study provides evidences that reveal the pharmacological mechanism of the Chinese plants with anti-diarrheal properties.

Original languageEnglish
Pages (from-to)60-66
Number of pages7
JournalJournal of Pharmacological Sciences
Volume94
Issue number1
DOIs
Publication statusPublished - 2004 Jan 1

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Ion Transport
Intestinal Mucosa
Medicinal Plants
Ethanol
Colforsin
Coptis
Sophora
Bumetanide
Plant Extracts
Ouabain
Epithelium
Pharmacology
kushen
qinpi

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Cite this

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abstract = "Effects of ethanol extracts of three Chinese medicinal plants, namely, Qinpi (Fraxini cortex), Kushen (Sophora flavescens, AITON), and Huanglian (Coptis teeta, WALLICH), on ion transport of the rat intestinal epithelia were determined in this study. Rat intestinal epithelia mounted in an Ussing chamber attached to a voltage/current clamp were used for measuring changes in the short circuit current across the epithelia. Activation of the intestinal epithelia by serosal administration of 5 μM forskolin resulted in an increase in basal short circuit current. The ethanol extracts of each of the three plants partially reduced the current stimulated by forskolin. In the following experiments, ouabain and bumetanide were added prior to adding the ethanol extract of these plants for revealing their effect on Na+ and Cl- movement. The results suggest that the ethanol extract of the Qinpi would affect Cl- transport. On the contrary, the ethanol extract of Kushen would affect Na+ transport rather than Cl - movement. This study provides evidences that reveal the pharmacological mechanism of the Chinese plants with anti-diarrheal properties.",
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Effect of Ethanol Extracts of Three Chinese Medicinal Plants with Anti-diarrheal Properties on Ion Transport of the Rat Intestinal Epithelia. / Tsai, Jong-Chang; Tsai, Shuli; Chang, Weng Cheng.

In: Journal of Pharmacological Sciences, Vol. 94, No. 1, 01.01.2004, p. 60-66.

Research output: Contribution to journalArticle

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