DNA stabilized silver nanoclusters as the fluorescent probe for studying the structural fluctuations and the solvation dynamics of human telomeric DNA

Hung Chi Hsu, Meng Chieh Ho, Kai Hung Wang, Ying Feng Hsu, Chih-Wei Chang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have prepared fluorescent silver nanoclusters (AgNCs) in the human telomeric sequence (d[AG3(T2AG3)3], Hum 22). There are two types of AgNCs that can be formed in Hum 22. The red emissive AgNCs (λem = 620 nm) are originated from the AgNCs attached onto the guanine bases, while the green emissive AgNCs (λem = 520 nm) are due to the AgNCs located between the adenine and guanine bases. We found that the fluorescence of the AgNCs is significantly quenched when Hum 22 forms a G-quadruplex structure. The fluorescence anisotropy decay dynamics and the time-resolved emission spectra of the AgNCs reveal the structural fluctuation and the solvation dynamics of Hum 22. The results indicate that the silver nanoclusters are an ideal fluorescent probe for human telomeric DNA and might be used as an important tool for studying the G-quadruplex structure. This journal is

Original languageEnglish
Pages (from-to)2140-2145
Number of pages6
JournalNew Journal of Chemistry
Volume39
Issue number3
DOIs
Publication statusPublished - 2015 Mar 1

Fingerprint

Nanoclusters
Solvation
Guanine
Fluorescent Dyes
Silver
DNA
Fluorescence
Adenine
Anisotropy

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Materials Chemistry

Cite this

@article{331a76f21308400ea5ef725990a182e0,
title = "DNA stabilized silver nanoclusters as the fluorescent probe for studying the structural fluctuations and the solvation dynamics of human telomeric DNA",
abstract = "We have prepared fluorescent silver nanoclusters (AgNCs) in the human telomeric sequence (d[AG3(T2AG3)3], Hum 22). There are two types of AgNCs that can be formed in Hum 22. The red emissive AgNCs (λem = 620 nm) are originated from the AgNCs attached onto the guanine bases, while the green emissive AgNCs (λem = 520 nm) are due to the AgNCs located between the adenine and guanine bases. We found that the fluorescence of the AgNCs is significantly quenched when Hum 22 forms a G-quadruplex structure. The fluorescence anisotropy decay dynamics and the time-resolved emission spectra of the AgNCs reveal the structural fluctuation and the solvation dynamics of Hum 22. The results indicate that the silver nanoclusters are an ideal fluorescent probe for human telomeric DNA and might be used as an important tool for studying the G-quadruplex structure. This journal is",
author = "Hsu, {Hung Chi} and Ho, {Meng Chieh} and Wang, {Kai Hung} and Hsu, {Ying Feng} and Chih-Wei Chang",
year = "2015",
month = "3",
day = "1",
doi = "10.1039/c4nj02065k",
language = "English",
volume = "39",
pages = "2140--2145",
journal = "New Journal of Chemistry",
issn = "1144-0546",
publisher = "Royal Society of Chemistry",
number = "3",

}

DNA stabilized silver nanoclusters as the fluorescent probe for studying the structural fluctuations and the solvation dynamics of human telomeric DNA. / Hsu, Hung Chi; Ho, Meng Chieh; Wang, Kai Hung; Hsu, Ying Feng; Chang, Chih-Wei.

In: New Journal of Chemistry, Vol. 39, No. 3, 01.03.2015, p. 2140-2145.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA stabilized silver nanoclusters as the fluorescent probe for studying the structural fluctuations and the solvation dynamics of human telomeric DNA

AU - Hsu, Hung Chi

AU - Ho, Meng Chieh

AU - Wang, Kai Hung

AU - Hsu, Ying Feng

AU - Chang, Chih-Wei

PY - 2015/3/1

Y1 - 2015/3/1

N2 - We have prepared fluorescent silver nanoclusters (AgNCs) in the human telomeric sequence (d[AG3(T2AG3)3], Hum 22). There are two types of AgNCs that can be formed in Hum 22. The red emissive AgNCs (λem = 620 nm) are originated from the AgNCs attached onto the guanine bases, while the green emissive AgNCs (λem = 520 nm) are due to the AgNCs located between the adenine and guanine bases. We found that the fluorescence of the AgNCs is significantly quenched when Hum 22 forms a G-quadruplex structure. The fluorescence anisotropy decay dynamics and the time-resolved emission spectra of the AgNCs reveal the structural fluctuation and the solvation dynamics of Hum 22. The results indicate that the silver nanoclusters are an ideal fluorescent probe for human telomeric DNA and might be used as an important tool for studying the G-quadruplex structure. This journal is

AB - We have prepared fluorescent silver nanoclusters (AgNCs) in the human telomeric sequence (d[AG3(T2AG3)3], Hum 22). There are two types of AgNCs that can be formed in Hum 22. The red emissive AgNCs (λem = 620 nm) are originated from the AgNCs attached onto the guanine bases, while the green emissive AgNCs (λem = 520 nm) are due to the AgNCs located between the adenine and guanine bases. We found that the fluorescence of the AgNCs is significantly quenched when Hum 22 forms a G-quadruplex structure. The fluorescence anisotropy decay dynamics and the time-resolved emission spectra of the AgNCs reveal the structural fluctuation and the solvation dynamics of Hum 22. The results indicate that the silver nanoclusters are an ideal fluorescent probe for human telomeric DNA and might be used as an important tool for studying the G-quadruplex structure. This journal is

UR - http://www.scopus.com/inward/record.url?scp=84924530490&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924530490&partnerID=8YFLogxK

U2 - 10.1039/c4nj02065k

DO - 10.1039/c4nj02065k

M3 - Article

AN - SCOPUS:84924530490

VL - 39

SP - 2140

EP - 2145

JO - New Journal of Chemistry

JF - New Journal of Chemistry

SN - 1144-0546

IS - 3

ER -