DNA engineered copper oxide-based nanocomposites with multiple enzyme-like activities for specific detection of mercury species in environmental and biological samples

Chia Wen Lien, Po Hsiung Yu, Huan Tsung Chang, Pang Hung Hsu, Tsunghsueh Wu, Yang Wei Lin, Chih Ching Huang, Jui Yang Lai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this paper, we report the synthesis and application of enzyme-like DNA-copper oxide/platinum nanoparticles for the separate quantification of inorganic and organomercury species in various real samples. We synthesized a series of poly(thymine) (T60)–copper oxide/metal nanocomposites (T60–CuxO/M NCs; M = Au, Ag or Pt) that exhibited enzyme-like activities [oxidase (OX), peroxidase (POX), and catalase (CAT)]. The enzyme-like activities are tunable due to the incorporation of various metals into the NCs. Among a series of synthesized CuxO/M NCs, T60–copper oxide-platinum nanocomposites (T60–CuxO/Pt NCs) exhibited the highest OX-like activity via the O2-mediated oxidation of substrates, such as Amplex Red (AR), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), o-phenylenediamine (OPD), and 3,3′,5,5′-tetramethylbenzidine (TMB), to form fluorescent or colored products. Interestingly, inorganic mercury ions (Hg2+) and organomercury species, such as methylmercury (MeHg+), ethylmercury (EtHg+), and phenylmercury (PhHg+), significantly inhibited the OX-like activity of T60–CuxO/Pt NCs. For the selective detection of mercury species, we used ABTS in the T60–CuxO/Pt NCs system, and the ABTS/T60–CuxO/Pt NCs-based assay allowed for the detection of mercury ions at nanomolar concentrations based on the decrease in the catalytic activity caused by the mercury ions. To separately quantify the inorganic and organomercury species in a sample, we employed selenium nanoparticles (Se NPs) as a masking agent, as they preferentially bind with inorganic mercury species. The ABTS/T60–CuxO/Pt NCs-based assay with the masking agent of Se NPs further provided specificity for the detection of organomercury species in environmental water samples (tap water, river water, and seawater) and fish muscle samples (dogfish muscle DORM-II).

Original languageEnglish
Pages (from-to)106-115
Number of pages10
JournalAnalytica Chimica Acta
Volume1084
DOIs
Publication statusPublished - 2019 Nov 25

Fingerprint

Nanocomposites
Copper oxides
Mercury
Oxides
Copper
oxide
enzyme
copper
DNA
Nanoparticles
Enzymes
Oxidoreductases
Ions
Selenium
Muscle
Water
Assays
selenium
platinum
Metals

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Lien, Chia Wen ; Yu, Po Hsiung ; Chang, Huan Tsung ; Hsu, Pang Hung ; Wu, Tsunghsueh ; Lin, Yang Wei ; Huang, Chih Ching ; Lai, Jui Yang. / DNA engineered copper oxide-based nanocomposites with multiple enzyme-like activities for specific detection of mercury species in environmental and biological samples. In: Analytica Chimica Acta. 2019 ; Vol. 1084. pp. 106-115.
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abstract = "In this paper, we report the synthesis and application of enzyme-like DNA-copper oxide/platinum nanoparticles for the separate quantification of inorganic and organomercury species in various real samples. We synthesized a series of poly(thymine) (T60)–copper oxide/metal nanocomposites (T60–CuxO/M NCs; M = Au, Ag or Pt) that exhibited enzyme-like activities [oxidase (OX), peroxidase (POX), and catalase (CAT)]. The enzyme-like activities are tunable due to the incorporation of various metals into the NCs. Among a series of synthesized CuxO/M NCs, T60–copper oxide-platinum nanocomposites (T60–CuxO/Pt NCs) exhibited the highest OX-like activity via the O2-mediated oxidation of substrates, such as Amplex Red (AR), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), o-phenylenediamine (OPD), and 3,3′,5,5′-tetramethylbenzidine (TMB), to form fluorescent or colored products. Interestingly, inorganic mercury ions (Hg2+) and organomercury species, such as methylmercury (MeHg+), ethylmercury (EtHg+), and phenylmercury (PhHg+), significantly inhibited the OX-like activity of T60–CuxO/Pt NCs. For the selective detection of mercury species, we used ABTS in the T60–CuxO/Pt NCs system, and the ABTS/T60–CuxO/Pt NCs-based assay allowed for the detection of mercury ions at nanomolar concentrations based on the decrease in the catalytic activity caused by the mercury ions. To separately quantify the inorganic and organomercury species in a sample, we employed selenium nanoparticles (Se NPs) as a masking agent, as they preferentially bind with inorganic mercury species. The ABTS/T60–CuxO/Pt NCs-based assay with the masking agent of Se NPs further provided specificity for the detection of organomercury species in environmental water samples (tap water, river water, and seawater) and fish muscle samples (dogfish muscle DORM-II).",
author = "Lien, {Chia Wen} and Yu, {Po Hsiung} and Chang, {Huan Tsung} and Hsu, {Pang Hung} and Tsunghsueh Wu and Lin, {Yang Wei} and Huang, {Chih Ching} and Lai, {Jui Yang}",
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DNA engineered copper oxide-based nanocomposites with multiple enzyme-like activities for specific detection of mercury species in environmental and biological samples. / Lien, Chia Wen; Yu, Po Hsiung; Chang, Huan Tsung; Hsu, Pang Hung; Wu, Tsunghsueh; Lin, Yang Wei; Huang, Chih Ching; Lai, Jui Yang.

In: Analytica Chimica Acta, Vol. 1084, 25.11.2019, p. 106-115.

Research output: Contribution to journalArticle

TY - JOUR

T1 - DNA engineered copper oxide-based nanocomposites with multiple enzyme-like activities for specific detection of mercury species in environmental and biological samples

AU - Lien, Chia Wen

AU - Yu, Po Hsiung

AU - Chang, Huan Tsung

AU - Hsu, Pang Hung

AU - Wu, Tsunghsueh

AU - Lin, Yang Wei

AU - Huang, Chih Ching

AU - Lai, Jui Yang

PY - 2019/11/25

Y1 - 2019/11/25

N2 - In this paper, we report the synthesis and application of enzyme-like DNA-copper oxide/platinum nanoparticles for the separate quantification of inorganic and organomercury species in various real samples. We synthesized a series of poly(thymine) (T60)–copper oxide/metal nanocomposites (T60–CuxO/M NCs; M = Au, Ag or Pt) that exhibited enzyme-like activities [oxidase (OX), peroxidase (POX), and catalase (CAT)]. The enzyme-like activities are tunable due to the incorporation of various metals into the NCs. Among a series of synthesized CuxO/M NCs, T60–copper oxide-platinum nanocomposites (T60–CuxO/Pt NCs) exhibited the highest OX-like activity via the O2-mediated oxidation of substrates, such as Amplex Red (AR), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), o-phenylenediamine (OPD), and 3,3′,5,5′-tetramethylbenzidine (TMB), to form fluorescent or colored products. Interestingly, inorganic mercury ions (Hg2+) and organomercury species, such as methylmercury (MeHg+), ethylmercury (EtHg+), and phenylmercury (PhHg+), significantly inhibited the OX-like activity of T60–CuxO/Pt NCs. For the selective detection of mercury species, we used ABTS in the T60–CuxO/Pt NCs system, and the ABTS/T60–CuxO/Pt NCs-based assay allowed for the detection of mercury ions at nanomolar concentrations based on the decrease in the catalytic activity caused by the mercury ions. To separately quantify the inorganic and organomercury species in a sample, we employed selenium nanoparticles (Se NPs) as a masking agent, as they preferentially bind with inorganic mercury species. The ABTS/T60–CuxO/Pt NCs-based assay with the masking agent of Se NPs further provided specificity for the detection of organomercury species in environmental water samples (tap water, river water, and seawater) and fish muscle samples (dogfish muscle DORM-II).

AB - In this paper, we report the synthesis and application of enzyme-like DNA-copper oxide/platinum nanoparticles for the separate quantification of inorganic and organomercury species in various real samples. We synthesized a series of poly(thymine) (T60)–copper oxide/metal nanocomposites (T60–CuxO/M NCs; M = Au, Ag or Pt) that exhibited enzyme-like activities [oxidase (OX), peroxidase (POX), and catalase (CAT)]. The enzyme-like activities are tunable due to the incorporation of various metals into the NCs. Among a series of synthesized CuxO/M NCs, T60–copper oxide-platinum nanocomposites (T60–CuxO/Pt NCs) exhibited the highest OX-like activity via the O2-mediated oxidation of substrates, such as Amplex Red (AR), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), o-phenylenediamine (OPD), and 3,3′,5,5′-tetramethylbenzidine (TMB), to form fluorescent or colored products. Interestingly, inorganic mercury ions (Hg2+) and organomercury species, such as methylmercury (MeHg+), ethylmercury (EtHg+), and phenylmercury (PhHg+), significantly inhibited the OX-like activity of T60–CuxO/Pt NCs. For the selective detection of mercury species, we used ABTS in the T60–CuxO/Pt NCs system, and the ABTS/T60–CuxO/Pt NCs-based assay allowed for the detection of mercury ions at nanomolar concentrations based on the decrease in the catalytic activity caused by the mercury ions. To separately quantify the inorganic and organomercury species in a sample, we employed selenium nanoparticles (Se NPs) as a masking agent, as they preferentially bind with inorganic mercury species. The ABTS/T60–CuxO/Pt NCs-based assay with the masking agent of Se NPs further provided specificity for the detection of organomercury species in environmental water samples (tap water, river water, and seawater) and fish muscle samples (dogfish muscle DORM-II).

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