Developmental changes in the level of vitellinimmunoreactive proteins in hemolymph and tissues of the blue crab Callinectes sapidus: Relation to vitellogenesis

Chi Ying Lee, Heidi R. Umphrey, R. Douglas Watson

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Abstract

Female blue crabs (Callinectes sapidus) were assigned to 1 of 6 stages based on oocyte development. An immunohistochemical study showed vitellin (Vn)-immunoreactivity was absent from ovaries in stages I and II, and was present in stages III, IV, V, and VI (postspawning). Vn-immunoreactivity was not detected in hepatopancreas, regardless of stage. Hemolymph vitellogenin (Vg) was quantified by enzyme-linked immunosorbent assay. Vg was not detected in hemolymph of stage I and II animals, but was detected in some animals in stages III, IV, V, and VI. The percentage of crabs having detectable hemolymph Vg was 17% (III), 32% (IV), 73% (V), and 100% (VI). Mean Vg levels were 0.02 ± 0.01 mg/ml (III), 0.02 ± 0.04 mg/ml (IV), 0.12 ± 0.15 mg/ml (V), and 0.14 ± 0.04 mg/ml (VI). The facts that Vg was present at low concentration in only a portion of the vitellogenic crabs, and that the level of Vg did not reach maximum until after spawning, are not consistent with the hypothesis that Vg is synthesized by extraovarian tissues and transported via hemolymph to the ovaries. It is possible that the presence of Vg in hemolymph may be a result of leakage of yolk proteins from the ovary due to oocyte reabsorption. The existence of atypical, possible atretic, oocytes in stage V and VI ovaries is consistent with this hypothesis. Considered together with the results of previous studies, these findings suggest that the ovary is the exclusive site of Vn synthesis in C. sapidus.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalJournal of Crustacean Biology
Volume16
Issue number1
Publication statusPublished - 1996 Feb 1

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Callinectes sapidus
vitellogenin
vitellogenesis
hemolymph
crab
crabs
protein
vitellin
proteins
animal
oocytes
leakage
spawning
assay
enzyme
yolk proteins
tissues
tissue
hepatopancreas
animals

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

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title = "Developmental changes in the level of vitellinimmunoreactive proteins in hemolymph and tissues of the blue crab Callinectes sapidus: Relation to vitellogenesis",
abstract = "Female blue crabs (Callinectes sapidus) were assigned to 1 of 6 stages based on oocyte development. An immunohistochemical study showed vitellin (Vn)-immunoreactivity was absent from ovaries in stages I and II, and was present in stages III, IV, V, and VI (postspawning). Vn-immunoreactivity was not detected in hepatopancreas, regardless of stage. Hemolymph vitellogenin (Vg) was quantified by enzyme-linked immunosorbent assay. Vg was not detected in hemolymph of stage I and II animals, but was detected in some animals in stages III, IV, V, and VI. The percentage of crabs having detectable hemolymph Vg was 17{\%} (III), 32{\%} (IV), 73{\%} (V), and 100{\%} (VI). Mean Vg levels were 0.02 ± 0.01 mg/ml (III), 0.02 ± 0.04 mg/ml (IV), 0.12 ± 0.15 mg/ml (V), and 0.14 ± 0.04 mg/ml (VI). The facts that Vg was present at low concentration in only a portion of the vitellogenic crabs, and that the level of Vg did not reach maximum until after spawning, are not consistent with the hypothesis that Vg is synthesized by extraovarian tissues and transported via hemolymph to the ovaries. It is possible that the presence of Vg in hemolymph may be a result of leakage of yolk proteins from the ovary due to oocyte reabsorption. The existence of atypical, possible atretic, oocytes in stage V and VI ovaries is consistent with this hypothesis. Considered together with the results of previous studies, these findings suggest that the ovary is the exclusive site of Vn synthesis in C. sapidus.",
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Developmental changes in the level of vitellinimmunoreactive proteins in hemolymph and tissues of the blue crab Callinectes sapidus : Relation to vitellogenesis. / Lee, Chi Ying; Umphrey, Heidi R.; Watson, R. Douglas.

In: Journal of Crustacean Biology, Vol. 16, No. 1, 01.02.1996, p. 1-9.

Research output: Contribution to journalArticle

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T1 - Developmental changes in the level of vitellinimmunoreactive proteins in hemolymph and tissues of the blue crab Callinectes sapidus

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AU - Lee, Chi Ying

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N2 - Female blue crabs (Callinectes sapidus) were assigned to 1 of 6 stages based on oocyte development. An immunohistochemical study showed vitellin (Vn)-immunoreactivity was absent from ovaries in stages I and II, and was present in stages III, IV, V, and VI (postspawning). Vn-immunoreactivity was not detected in hepatopancreas, regardless of stage. Hemolymph vitellogenin (Vg) was quantified by enzyme-linked immunosorbent assay. Vg was not detected in hemolymph of stage I and II animals, but was detected in some animals in stages III, IV, V, and VI. The percentage of crabs having detectable hemolymph Vg was 17% (III), 32% (IV), 73% (V), and 100% (VI). Mean Vg levels were 0.02 ± 0.01 mg/ml (III), 0.02 ± 0.04 mg/ml (IV), 0.12 ± 0.15 mg/ml (V), and 0.14 ± 0.04 mg/ml (VI). The facts that Vg was present at low concentration in only a portion of the vitellogenic crabs, and that the level of Vg did not reach maximum until after spawning, are not consistent with the hypothesis that Vg is synthesized by extraovarian tissues and transported via hemolymph to the ovaries. It is possible that the presence of Vg in hemolymph may be a result of leakage of yolk proteins from the ovary due to oocyte reabsorption. The existence of atypical, possible atretic, oocytes in stage V and VI ovaries is consistent with this hypothesis. Considered together with the results of previous studies, these findings suggest that the ovary is the exclusive site of Vn synthesis in C. sapidus.

AB - Female blue crabs (Callinectes sapidus) were assigned to 1 of 6 stages based on oocyte development. An immunohistochemical study showed vitellin (Vn)-immunoreactivity was absent from ovaries in stages I and II, and was present in stages III, IV, V, and VI (postspawning). Vn-immunoreactivity was not detected in hepatopancreas, regardless of stage. Hemolymph vitellogenin (Vg) was quantified by enzyme-linked immunosorbent assay. Vg was not detected in hemolymph of stage I and II animals, but was detected in some animals in stages III, IV, V, and VI. The percentage of crabs having detectable hemolymph Vg was 17% (III), 32% (IV), 73% (V), and 100% (VI). Mean Vg levels were 0.02 ± 0.01 mg/ml (III), 0.02 ± 0.04 mg/ml (IV), 0.12 ± 0.15 mg/ml (V), and 0.14 ± 0.04 mg/ml (VI). The facts that Vg was present at low concentration in only a portion of the vitellogenic crabs, and that the level of Vg did not reach maximum until after spawning, are not consistent with the hypothesis that Vg is synthesized by extraovarian tissues and transported via hemolymph to the ovaries. It is possible that the presence of Vg in hemolymph may be a result of leakage of yolk proteins from the ovary due to oocyte reabsorption. The existence of atypical, possible atretic, oocytes in stage V and VI ovaries is consistent with this hypothesis. Considered together with the results of previous studies, these findings suggest that the ovary is the exclusive site of Vn synthesis in C. sapidus.

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