Development of primary cell cultures from the adult xylem-feeding leafhopper, Kolla paulula, as a tool for studying Wolbachia biology

Rong Jen Shiau, Hsien Tzung Shih, Shin Yi Chen, Chiou Chu Su, Wei Huang Tsai, Yu-Der Wen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Xylophagous leafhoppers are vectors of xylem-limited plant pathogens such as citrus variegated chlorosis (CVC) and Pierce's disease, which cause Xylella diseases. Currently, no cure for Xylella diseases exists. The objective of endosymbiont control using Wolbachia pipientis is to reduce the populations of insect vectors, potentially preventing the expansion of Xylella diseases. The purpose of this study was to establish primary cell cultures from adult xylem-feeding leafhoppers to study Wolbachia biology. Cells from adult male and female Kolla paulula (Walker) (Hemiptera: Membracoidea: Cicadellinae) were successfully cultured in Dulbecco's Modified Eagle Medium containing 20% fetal bovine serum and were maintained for more than 6 months. Cells of both male and female adults are round and semi-attached. The doubling times for male and female K. paulula cells are approximately 8 and 10 days, respectively. The presence of Wolbachia in K. paulula cell cultures was detected by polymerase chain reaction (PCR) amplification of Wolbachia surface protein (wsp) gene. The cell cultures developed in this study may be useful in studying interactions between Wolbachia and its hosts of different genders.

Original languageEnglish
Pages (from-to)503-507
Number of pages5
JournalJournal of Asia-Pacific Entomology
Volume14
Issue number4
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Xylella
Wolbachia
Cicadellidae
xylem
cell culture
Biological Sciences
Membracoidea
citrus variegated chlorosis
Wolbachia pipientis
Pierce's disease
insect vectors
fetal bovine serum
surface proteins
endosymbionts
eagles
cells
plant pathogens
Hemiptera
polymerase chain reaction
gender

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

Shiau, Rong Jen ; Shih, Hsien Tzung ; Chen, Shin Yi ; Su, Chiou Chu ; Tsai, Wei Huang ; Wen, Yu-Der. / Development of primary cell cultures from the adult xylem-feeding leafhopper, Kolla paulula, as a tool for studying Wolbachia biology. In: Journal of Asia-Pacific Entomology. 2011 ; Vol. 14, No. 4. pp. 503-507.
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abstract = "Xylophagous leafhoppers are vectors of xylem-limited plant pathogens such as citrus variegated chlorosis (CVC) and Pierce's disease, which cause Xylella diseases. Currently, no cure for Xylella diseases exists. The objective of endosymbiont control using Wolbachia pipientis is to reduce the populations of insect vectors, potentially preventing the expansion of Xylella diseases. The purpose of this study was to establish primary cell cultures from adult xylem-feeding leafhoppers to study Wolbachia biology. Cells from adult male and female Kolla paulula (Walker) (Hemiptera: Membracoidea: Cicadellinae) were successfully cultured in Dulbecco's Modified Eagle Medium containing 20{\%} fetal bovine serum and were maintained for more than 6 months. Cells of both male and female adults are round and semi-attached. The doubling times for male and female K. paulula cells are approximately 8 and 10 days, respectively. The presence of Wolbachia in K. paulula cell cultures was detected by polymerase chain reaction (PCR) amplification of Wolbachia surface protein (wsp) gene. The cell cultures developed in this study may be useful in studying interactions between Wolbachia and its hosts of different genders.",
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Development of primary cell cultures from the adult xylem-feeding leafhopper, Kolla paulula, as a tool for studying Wolbachia biology. / Shiau, Rong Jen; Shih, Hsien Tzung; Chen, Shin Yi; Su, Chiou Chu; Tsai, Wei Huang; Wen, Yu-Der.

In: Journal of Asia-Pacific Entomology, Vol. 14, No. 4, 01.12.2011, p. 503-507.

Research output: Contribution to journalArticle

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