Abstract
Xylophagous leafhoppers are vectors of xylem-limited plant pathogens such as citrus variegated chlorosis (CVC) and Pierce's disease, which cause Xylella diseases. Currently, no cure for Xylella diseases exists. The objective of endosymbiont control using Wolbachia pipientis is to reduce the populations of insect vectors, potentially preventing the expansion of Xylella diseases. The purpose of this study was to establish primary cell cultures from adult xylem-feeding leafhoppers to study Wolbachia biology. Cells from adult male and female Kolla paulula (Walker) (Hemiptera: Membracoidea: Cicadellinae) were successfully cultured in Dulbecco's Modified Eagle Medium containing 20% fetal bovine serum and were maintained for more than 6 months. Cells of both male and female adults are round and semi-attached. The doubling times for male and female K. paulula cells are approximately 8 and 10 days, respectively. The presence of Wolbachia in K. paulula cell cultures was detected by polymerase chain reaction (PCR) amplification of Wolbachia surface protein (wsp) gene. The cell cultures developed in this study may be useful in studying interactions between Wolbachia and its hosts of different genders.
| Original language | English |
|---|---|
| Pages (from-to) | 503-507 |
| Number of pages | 5 |
| Journal | Journal of Asia-Pacific Entomology |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2011 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Insect Science
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Development of primary cell cultures from the adult xylem-feeding leafhopper, Kolla paulula, as a tool for studying Wolbachia biology. / Shiau, Rong Jen; Shih, Hsien Tzung; Chen, Shin Yi; Su, Chiou Chu; Tsai, Wei Huang; Wen, Yu-Der.
In: Journal of Asia-Pacific Entomology, Vol. 14, No. 4, 01.12.2011, p. 503-507.Research output: Contribution to journal › Article
TY - JOUR
T1 - Development of primary cell cultures from the adult xylem-feeding leafhopper, Kolla paulula, as a tool for studying Wolbachia biology
AU - Shiau, Rong Jen
AU - Shih, Hsien Tzung
AU - Chen, Shin Yi
AU - Su, Chiou Chu
AU - Tsai, Wei Huang
AU - Wen, Yu-Der
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Xylophagous leafhoppers are vectors of xylem-limited plant pathogens such as citrus variegated chlorosis (CVC) and Pierce's disease, which cause Xylella diseases. Currently, no cure for Xylella diseases exists. The objective of endosymbiont control using Wolbachia pipientis is to reduce the populations of insect vectors, potentially preventing the expansion of Xylella diseases. The purpose of this study was to establish primary cell cultures from adult xylem-feeding leafhoppers to study Wolbachia biology. Cells from adult male and female Kolla paulula (Walker) (Hemiptera: Membracoidea: Cicadellinae) were successfully cultured in Dulbecco's Modified Eagle Medium containing 20% fetal bovine serum and were maintained for more than 6 months. Cells of both male and female adults are round and semi-attached. The doubling times for male and female K. paulula cells are approximately 8 and 10 days, respectively. The presence of Wolbachia in K. paulula cell cultures was detected by polymerase chain reaction (PCR) amplification of Wolbachia surface protein (wsp) gene. The cell cultures developed in this study may be useful in studying interactions between Wolbachia and its hosts of different genders.
AB - Xylophagous leafhoppers are vectors of xylem-limited plant pathogens such as citrus variegated chlorosis (CVC) and Pierce's disease, which cause Xylella diseases. Currently, no cure for Xylella diseases exists. The objective of endosymbiont control using Wolbachia pipientis is to reduce the populations of insect vectors, potentially preventing the expansion of Xylella diseases. The purpose of this study was to establish primary cell cultures from adult xylem-feeding leafhoppers to study Wolbachia biology. Cells from adult male and female Kolla paulula (Walker) (Hemiptera: Membracoidea: Cicadellinae) were successfully cultured in Dulbecco's Modified Eagle Medium containing 20% fetal bovine serum and were maintained for more than 6 months. Cells of both male and female adults are round and semi-attached. The doubling times for male and female K. paulula cells are approximately 8 and 10 days, respectively. The presence of Wolbachia in K. paulula cell cultures was detected by polymerase chain reaction (PCR) amplification of Wolbachia surface protein (wsp) gene. The cell cultures developed in this study may be useful in studying interactions between Wolbachia and its hosts of different genders.
UR - http://www.scopus.com/inward/record.url?scp=80051905477&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051905477&partnerID=8YFLogxK
U2 - 10.1016/j.aspen.2011.07.011
DO - 10.1016/j.aspen.2011.07.011
M3 - Article
AN - SCOPUS:80051905477
VL - 14
SP - 503
EP - 507
JO - Journal of Asia-Pacific Entomology
JF - Journal of Asia-Pacific Entomology
SN - 1226-8615
IS - 4
ER -