Determination of tertiary amines based on pH junctions and field amplification in capillary electrophoresis with electrochemiluminescence detection

Mallipattu Sreedhar, Yang-Wei Lin, Wei Lung Tseng, Huan Tsung Chang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A stacking approach based on pH junction and field amplification has been developed for determining amines by capillary electrophoresis (CE) with electrochemiluminescence (ECL) detection. A two-electrode configuration was employed with an indium/tin oxide-coated glass as a working electrode and a platinum wire as a pseudoreference electrode. The ECL system also contains a flow cell (poly(dimethylsiloxane)-aluminum oxide) that was made from a mixture of Sylgard 184 silicone elastomer, a curing agent, and aluminum oxide. In order to improve the sensitivity of the present CE-ECL system using tris(2,2′-bipyridyl) ruthenium(II) (Ru(bpy)32+), a stacking approach based on pH junctions and field amplification has been tested for the analysis of triethylamine (TEA), tripropylamine (TPA), and tributylamine (TBA). Once amines (cations) prepared in citric acid solution (pH < 4.0) migrate towards the background electrolyte (15 mM sodium borate at pH 8.0), they slow down and are stacked at the boundary as a result of deprotonation and decreases in the electric field. By applying hydrodynamic injection of the sample for 60 s, this method provides the concentration limits of detection (signal-to-noise ratio = 3) of 24, 20, and 32 nM for TEA, TPA, and TBA, respectively. The results indicate that the stacking CE-ECL system is better than CE-ECL systems using a two-electrode configuration and comparable to those using a three-electrode configuration. The potential applicability of the new and low-cost CE-ECL system has been demonstrated by the determination of 1.0 μM lidocaine, a local anesthetic drug, in urine without any tedious sample preparation.

Original languageEnglish
Pages (from-to)2984-2990
Number of pages7
JournalElectrophoresis
Volume26
Issue number15
DOIs
Publication statusPublished - 2005 Aug 1

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Amines
Amplification
Electrodes
Aluminum Oxide
Silicone Elastomers
2,2'-Dipyridyl
Deprotonation
Ruthenium
Signal-To-Noise Ratio
Hydrodynamics
Lidocaine
Local Anesthetics
Platinum
Citric Acid
Electrolytes
Glass
Curing
Anesthetics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry

Cite this

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title = "Determination of tertiary amines based on pH junctions and field amplification in capillary electrophoresis with electrochemiluminescence detection",
abstract = "A stacking approach based on pH junction and field amplification has been developed for determining amines by capillary electrophoresis (CE) with electrochemiluminescence (ECL) detection. A two-electrode configuration was employed with an indium/tin oxide-coated glass as a working electrode and a platinum wire as a pseudoreference electrode. The ECL system also contains a flow cell (poly(dimethylsiloxane)-aluminum oxide) that was made from a mixture of Sylgard 184 silicone elastomer, a curing agent, and aluminum oxide. In order to improve the sensitivity of the present CE-ECL system using tris(2,2′-bipyridyl) ruthenium(II) (Ru(bpy)32+), a stacking approach based on pH junctions and field amplification has been tested for the analysis of triethylamine (TEA), tripropylamine (TPA), and tributylamine (TBA). Once amines (cations) prepared in citric acid solution (pH < 4.0) migrate towards the background electrolyte (15 mM sodium borate at pH 8.0), they slow down and are stacked at the boundary as a result of deprotonation and decreases in the electric field. By applying hydrodynamic injection of the sample for 60 s, this method provides the concentration limits of detection (signal-to-noise ratio = 3) of 24, 20, and 32 nM for TEA, TPA, and TBA, respectively. The results indicate that the stacking CE-ECL system is better than CE-ECL systems using a two-electrode configuration and comparable to those using a three-electrode configuration. The potential applicability of the new and low-cost CE-ECL system has been demonstrated by the determination of 1.0 μM lidocaine, a local anesthetic drug, in urine without any tedious sample preparation.",
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Determination of tertiary amines based on pH junctions and field amplification in capillary electrophoresis with electrochemiluminescence detection. / Sreedhar, Mallipattu; Lin, Yang-Wei; Tseng, Wei Lung; Chang, Huan Tsung.

In: Electrophoresis, Vol. 26, No. 15, 01.08.2005, p. 2984-2990.

Research output: Contribution to journalArticle

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