Abstract
In this study, a magnetic iron oxide nanoparticle-based solid-phase extraction procedure combined with the online concentration and separation of salicylic acid (SA) through micellar electrokinetic chromatography–UV detection (MEKC–UV) was developed. Under optimal experimental conditions, a good linearity in the range of 0.01–100 μmol L−1 was obtained with a coefficient of correlation of 0.9999. The detection sensitivity of the proposed method exhibited an approximately 1026-fold improvement compared with a single MEKC method without online concentration, and the detection limit (S/N = 3) was 3.80 nmol L−1. The repeatability of the method was evaluated using intraday and interday RSDs (11.5% and 17.0%, respectively). The method was used to determine SA concentrations in tobacco leaves (Nicotiana tabacum L. cv. Samsun) from the NN genotype, nn genotype, and Nt-NahG mutant strains, as well as in shampoo and ointment samples. Rapid extraction and separation ( < 50 min), acceptable repeatability (RSD < 17.0%), and high spiked recoveries (95.8%–102.4%) were observed for plants, detergents, and pharmaceuticals.
| Original language | English |
|---|---|
| Pages (from-to) | 62-70 |
| Number of pages | 9 |
| Journal | Journal of Chromatography A |
| Volume | 1479 |
| DOIs | |
| Publication status | Published - 2017 Jan 6 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
Cite this
}
Determination of salicylic acid using a magnetic iron oxide nanoparticle-based solid-phase extraction procedure followed by an online concentration technique through micellar electrokinetic capillary chromatography. / Chang, Yu Hsuan; Huang, Chang Wei; Fu, Shih-Feng; Wu, Mei Yao; Wu, Tsunghsueh; Lin, Yang-Wei.
In: Journal of Chromatography A, Vol. 1479, 06.01.2017, p. 62-70.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination of salicylic acid using a magnetic iron oxide nanoparticle-based solid-phase extraction procedure followed by an online concentration technique through micellar electrokinetic capillary chromatography
AU - Chang, Yu Hsuan
AU - Huang, Chang Wei
AU - Fu, Shih-Feng
AU - Wu, Mei Yao
AU - Wu, Tsunghsueh
AU - Lin, Yang-Wei
PY - 2017/1/6
Y1 - 2017/1/6
N2 - In this study, a magnetic iron oxide nanoparticle-based solid-phase extraction procedure combined with the online concentration and separation of salicylic acid (SA) through micellar electrokinetic chromatography–UV detection (MEKC–UV) was developed. Under optimal experimental conditions, a good linearity in the range of 0.01–100 μmol L−1 was obtained with a coefficient of correlation of 0.9999. The detection sensitivity of the proposed method exhibited an approximately 1026-fold improvement compared with a single MEKC method without online concentration, and the detection limit (S/N = 3) was 3.80 nmol L−1. The repeatability of the method was evaluated using intraday and interday RSDs (11.5% and 17.0%, respectively). The method was used to determine SA concentrations in tobacco leaves (Nicotiana tabacum L. cv. Samsun) from the NN genotype, nn genotype, and Nt-NahG mutant strains, as well as in shampoo and ointment samples. Rapid extraction and separation ( < 50 min), acceptable repeatability (RSD < 17.0%), and high spiked recoveries (95.8%–102.4%) were observed for plants, detergents, and pharmaceuticals.
AB - In this study, a magnetic iron oxide nanoparticle-based solid-phase extraction procedure combined with the online concentration and separation of salicylic acid (SA) through micellar electrokinetic chromatography–UV detection (MEKC–UV) was developed. Under optimal experimental conditions, a good linearity in the range of 0.01–100 μmol L−1 was obtained with a coefficient of correlation of 0.9999. The detection sensitivity of the proposed method exhibited an approximately 1026-fold improvement compared with a single MEKC method without online concentration, and the detection limit (S/N = 3) was 3.80 nmol L−1. The repeatability of the method was evaluated using intraday and interday RSDs (11.5% and 17.0%, respectively). The method was used to determine SA concentrations in tobacco leaves (Nicotiana tabacum L. cv. Samsun) from the NN genotype, nn genotype, and Nt-NahG mutant strains, as well as in shampoo and ointment samples. Rapid extraction and separation ( < 50 min), acceptable repeatability (RSD < 17.0%), and high spiked recoveries (95.8%–102.4%) were observed for plants, detergents, and pharmaceuticals.
UR - http://www.scopus.com/inward/record.url?scp=85008263049&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85008263049&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2016.12.018
DO - 10.1016/j.chroma.2016.12.018
M3 - Article
C2 - 27988078
AN - SCOPUS:85008263049
VL - 1479
SP - 62
EP - 70
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
ER -