TY - JOUR
T1 - Determination of salicylic acid using a magnetic iron oxide nanoparticle-based solid-phase extraction procedure followed by an online concentration technique through micellar electrokinetic capillary chromatography
AU - Chang, Yu Hsuan
AU - Huang, Chang Wei
AU - Fu, Shih Feng
AU - Wu, Mei Yao
AU - Wu, Tsunghsueh
AU - Lin, Yang Wei
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/1/6
Y1 - 2017/1/6
N2 - In this study, a magnetic iron oxide nanoparticle-based solid-phase extraction procedure combined with the online concentration and separation of salicylic acid (SA) through micellar electrokinetic chromatography–UV detection (MEKC–UV) was developed. Under optimal experimental conditions, a good linearity in the range of 0.01–100 μmol L−1 was obtained with a coefficient of correlation of 0.9999. The detection sensitivity of the proposed method exhibited an approximately 1026-fold improvement compared with a single MEKC method without online concentration, and the detection limit (S/N = 3) was 3.80 nmol L−1. The repeatability of the method was evaluated using intraday and interday RSDs (11.5% and 17.0%, respectively). The method was used to determine SA concentrations in tobacco leaves (Nicotiana tabacum L. cv. Samsun) from the NN genotype, nn genotype, and Nt-NahG mutant strains, as well as in shampoo and ointment samples. Rapid extraction and separation ( < 50 min), acceptable repeatability (RSD < 17.0%), and high spiked recoveries (95.8%–102.4%) were observed for plants, detergents, and pharmaceuticals.
AB - In this study, a magnetic iron oxide nanoparticle-based solid-phase extraction procedure combined with the online concentration and separation of salicylic acid (SA) through micellar electrokinetic chromatography–UV detection (MEKC–UV) was developed. Under optimal experimental conditions, a good linearity in the range of 0.01–100 μmol L−1 was obtained with a coefficient of correlation of 0.9999. The detection sensitivity of the proposed method exhibited an approximately 1026-fold improvement compared with a single MEKC method without online concentration, and the detection limit (S/N = 3) was 3.80 nmol L−1. The repeatability of the method was evaluated using intraday and interday RSDs (11.5% and 17.0%, respectively). The method was used to determine SA concentrations in tobacco leaves (Nicotiana tabacum L. cv. Samsun) from the NN genotype, nn genotype, and Nt-NahG mutant strains, as well as in shampoo and ointment samples. Rapid extraction and separation ( < 50 min), acceptable repeatability (RSD < 17.0%), and high spiked recoveries (95.8%–102.4%) were observed for plants, detergents, and pharmaceuticals.
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U2 - 10.1016/j.chroma.2016.12.018
DO - 10.1016/j.chroma.2016.12.018
M3 - Article
C2 - 27988078
AN - SCOPUS:85008263049
VL - 1479
SP - 62
EP - 70
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -