Detection of proteins and protein-ligand complexes using hgte nanostructure matrixes in surface-assisted laser desorption/ionization mass spectrometry

Cheng Kang Chiang, Zusing Yang, Yang Wei Lin, Wen Tsen Chen, Han Jia Lin, Huan Tsung Chang

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We have analyzed peptides, proteins, and protein-drug complexes through surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using HgTe nanostructures as matrixes. We investigated the effects of several parameters, including the concentration of the HgTe nanostructures, the pH of the buffer, and the concentration of salt, on the performance of this system. When HgTe nanostructures are used as matrixes, [M + H]+ ions were the dominant signals. Relative to other commonly used nanomaterials, HgTe nanostructures provided lower background signals from metal clusters, fewer fragment ions, less interference from alkali-adducted analyte ions, and a higher mass range (up to 150-000 Da). The present approach provides limits of detection for angiotensin I and bovine serum albumin of 200 pM and 14 nM, respectively, with great reproducibility (RSD: <25%). We validated the applicability of this method through the detections of (i) the recombinant proteins that were transformed in E. coli, (ii) the specific complex between bovine serum albumin and l-tryptophan, and (iii) a carbonic anhydrase- acetazolamide complex. Our results suggest that this novel and simple SALDI-MS approach using HgTe nanostructures as matrixes might open several new ways for proteomics and the analysis of drug-protein complexes.

Original languageEnglish
Pages (from-to)4543-4550
Number of pages8
JournalAnalytical Chemistry
Volume82
Issue number11
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Ionization
Mass spectrometry
Nanostructures
Desorption
Ligands
Lasers
Ions
Bovine Serum Albumin
Proteins
Angiotensin I
Acetazolamide
Carbonic Anhydrases
Alkalies
Recombinant Proteins
Nanostructured materials
Tryptophan
Pharmaceutical Preparations
Escherichia coli
Buffers
Salts

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Chiang, Cheng Kang ; Yang, Zusing ; Lin, Yang Wei ; Chen, Wen Tsen ; Lin, Han Jia ; Chang, Huan Tsung. / Detection of proteins and protein-ligand complexes using hgte nanostructure matrixes in surface-assisted laser desorption/ionization mass spectrometry. In: Analytical Chemistry. 2010 ; Vol. 82, No. 11. pp. 4543-4550.
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Detection of proteins and protein-ligand complexes using hgte nanostructure matrixes in surface-assisted laser desorption/ionization mass spectrometry. / Chiang, Cheng Kang; Yang, Zusing; Lin, Yang Wei; Chen, Wen Tsen; Lin, Han Jia; Chang, Huan Tsung.

In: Analytical Chemistry, Vol. 82, No. 11, 01.06.2010, p. 4543-4550.

Research output: Contribution to journalArticle

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