Detection of mercury and phenylmercury ions using DNA-based fluorescent probe

Yang Wei Lin, Huan Tsung Chang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A DNA probe labeled with a 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) quencher and a carboxyfluorescein (FAM) donor at its 5′- and 3′-termini can be used for the detection of Hg2+ ions and phenylmercury ions (PhHg+). This DNA probe possesses a random coil structure that changes into a hairpin-like structure upon binding Hg 2+ and PhHg+ ions. As a result, the fluorescence of the FAM unit decreased through quenching between the donor and the quencher. In the presence of ethylenediaminetetraacetic acid (EDTA), the DNA probe allowed the selective detection of PhHg+ ions at concentrations as low as 70.0 nM, mainly as a result of T-Hg2+-T coordination and π-π stacking between the Ph unit and DNA bases. A linear correlation existed between the fluorescence intensity and the concentration of PhHg+ ions over the range from 0.10 to 1.0 μM (R2 = 0.99). After acid hydrolysis and neutralization of the samples, all of the mercury species are converted to Hg2+ ions, allowing us to use the DNA-based probe to determine the concentrations of total mercury species at the nM level. The practicality of this probe has been validated by the analyses of pond water and fish samples, showing its advantages of sensitivity, selectivity, and simplicity.

Original languageEnglish
Pages (from-to)3323-3328
Number of pages6
JournalAnalyst
Volume136
Issue number16
DOIs
Publication statusPublished - 2011 Aug 21

Fingerprint

Mercury
Fluorescent Dyes
DNA
DNA Probes
probe
Ions
ion
fluorescence
Fluorescence
Benzoic Acid
Benzoic acid
acid
Ethylenediaminetetraacetic acid
Ponds
neutralization
EDTA
stacking
Edetic Acid
Fish
mercury

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Lin, Yang Wei ; Chang, Huan Tsung. / Detection of mercury and phenylmercury ions using DNA-based fluorescent probe. In: Analyst. 2011 ; Vol. 136, No. 16. pp. 3323-3328.
@article{940d5b1570e742a8a43cc2e3c3e75b69,
title = "Detection of mercury and phenylmercury ions using DNA-based fluorescent probe",
abstract = "A DNA probe labeled with a 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) quencher and a carboxyfluorescein (FAM) donor at its 5′- and 3′-termini can be used for the detection of Hg2+ ions and phenylmercury ions (PhHg+). This DNA probe possesses a random coil structure that changes into a hairpin-like structure upon binding Hg 2+ and PhHg+ ions. As a result, the fluorescence of the FAM unit decreased through quenching between the donor and the quencher. In the presence of ethylenediaminetetraacetic acid (EDTA), the DNA probe allowed the selective detection of PhHg+ ions at concentrations as low as 70.0 nM, mainly as a result of T-Hg2+-T coordination and π-π stacking between the Ph unit and DNA bases. A linear correlation existed between the fluorescence intensity and the concentration of PhHg+ ions over the range from 0.10 to 1.0 μM (R2 = 0.99). After acid hydrolysis and neutralization of the samples, all of the mercury species are converted to Hg2+ ions, allowing us to use the DNA-based probe to determine the concentrations of total mercury species at the nM level. The practicality of this probe has been validated by the analyses of pond water and fish samples, showing its advantages of sensitivity, selectivity, and simplicity.",
author = "Lin, {Yang Wei} and Chang, {Huan Tsung}",
year = "2011",
month = "8",
day = "21",
doi = "10.1039/c1an15117g",
language = "English",
volume = "136",
pages = "3323--3328",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "16",

}

Detection of mercury and phenylmercury ions using DNA-based fluorescent probe. / Lin, Yang Wei; Chang, Huan Tsung.

In: Analyst, Vol. 136, No. 16, 21.08.2011, p. 3323-3328.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of mercury and phenylmercury ions using DNA-based fluorescent probe

AU - Lin, Yang Wei

AU - Chang, Huan Tsung

PY - 2011/8/21

Y1 - 2011/8/21

N2 - A DNA probe labeled with a 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) quencher and a carboxyfluorescein (FAM) donor at its 5′- and 3′-termini can be used for the detection of Hg2+ ions and phenylmercury ions (PhHg+). This DNA probe possesses a random coil structure that changes into a hairpin-like structure upon binding Hg 2+ and PhHg+ ions. As a result, the fluorescence of the FAM unit decreased through quenching between the donor and the quencher. In the presence of ethylenediaminetetraacetic acid (EDTA), the DNA probe allowed the selective detection of PhHg+ ions at concentrations as low as 70.0 nM, mainly as a result of T-Hg2+-T coordination and π-π stacking between the Ph unit and DNA bases. A linear correlation existed between the fluorescence intensity and the concentration of PhHg+ ions over the range from 0.10 to 1.0 μM (R2 = 0.99). After acid hydrolysis and neutralization of the samples, all of the mercury species are converted to Hg2+ ions, allowing us to use the DNA-based probe to determine the concentrations of total mercury species at the nM level. The practicality of this probe has been validated by the analyses of pond water and fish samples, showing its advantages of sensitivity, selectivity, and simplicity.

AB - A DNA probe labeled with a 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) quencher and a carboxyfluorescein (FAM) donor at its 5′- and 3′-termini can be used for the detection of Hg2+ ions and phenylmercury ions (PhHg+). This DNA probe possesses a random coil structure that changes into a hairpin-like structure upon binding Hg 2+ and PhHg+ ions. As a result, the fluorescence of the FAM unit decreased through quenching between the donor and the quencher. In the presence of ethylenediaminetetraacetic acid (EDTA), the DNA probe allowed the selective detection of PhHg+ ions at concentrations as low as 70.0 nM, mainly as a result of T-Hg2+-T coordination and π-π stacking between the Ph unit and DNA bases. A linear correlation existed between the fluorescence intensity and the concentration of PhHg+ ions over the range from 0.10 to 1.0 μM (R2 = 0.99). After acid hydrolysis and neutralization of the samples, all of the mercury species are converted to Hg2+ ions, allowing us to use the DNA-based probe to determine the concentrations of total mercury species at the nM level. The practicality of this probe has been validated by the analyses of pond water and fish samples, showing its advantages of sensitivity, selectivity, and simplicity.

UR - http://www.scopus.com/inward/record.url?scp=79960933060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960933060&partnerID=8YFLogxK

U2 - 10.1039/c1an15117g

DO - 10.1039/c1an15117g

M3 - Article

VL - 136

SP - 3323

EP - 3328

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 16

ER -