Abstract
A DNA probe labeled with a 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) quencher and a carboxyfluorescein (FAM) donor at its 5′- and 3′-termini can be used for the detection of Hg2+ ions and phenylmercury ions (PhHg+). This DNA probe possesses a random coil structure that changes into a hairpin-like structure upon binding Hg 2+ and PhHg+ ions. As a result, the fluorescence of the FAM unit decreased through quenching between the donor and the quencher. In the presence of ethylenediaminetetraacetic acid (EDTA), the DNA probe allowed the selective detection of PhHg+ ions at concentrations as low as 70.0 nM, mainly as a result of T-Hg2+-T coordination and π-π stacking between the Ph unit and DNA bases. A linear correlation existed between the fluorescence intensity and the concentration of PhHg+ ions over the range from 0.10 to 1.0 μM (R2 = 0.99). After acid hydrolysis and neutralization of the samples, all of the mercury species are converted to Hg2+ ions, allowing us to use the DNA-based probe to determine the concentrations of total mercury species at the nM level. The practicality of this probe has been validated by the analyses of pond water and fish samples, showing its advantages of sensitivity, selectivity, and simplicity.
| Original language | English |
|---|---|
| Pages (from-to) | 3323-3328 |
| Number of pages | 6 |
| Journal | Analyst |
| Volume | 136 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 2011 Aug 21 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry
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Detection of mercury and phenylmercury ions using DNA-based fluorescent probe. / Lin, Yang Wei; Chang, Huan Tsung.
In: Analyst, Vol. 136, No. 16, 21.08.2011, p. 3323-3328.Research output: Contribution to journal › Article
TY - JOUR
T1 - Detection of mercury and phenylmercury ions using DNA-based fluorescent probe
AU - Lin, Yang Wei
AU - Chang, Huan Tsung
PY - 2011/8/21
Y1 - 2011/8/21
N2 - A DNA probe labeled with a 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) quencher and a carboxyfluorescein (FAM) donor at its 5′- and 3′-termini can be used for the detection of Hg2+ ions and phenylmercury ions (PhHg+). This DNA probe possesses a random coil structure that changes into a hairpin-like structure upon binding Hg 2+ and PhHg+ ions. As a result, the fluorescence of the FAM unit decreased through quenching between the donor and the quencher. In the presence of ethylenediaminetetraacetic acid (EDTA), the DNA probe allowed the selective detection of PhHg+ ions at concentrations as low as 70.0 nM, mainly as a result of T-Hg2+-T coordination and π-π stacking between the Ph unit and DNA bases. A linear correlation existed between the fluorescence intensity and the concentration of PhHg+ ions over the range from 0.10 to 1.0 μM (R2 = 0.99). After acid hydrolysis and neutralization of the samples, all of the mercury species are converted to Hg2+ ions, allowing us to use the DNA-based probe to determine the concentrations of total mercury species at the nM level. The practicality of this probe has been validated by the analyses of pond water and fish samples, showing its advantages of sensitivity, selectivity, and simplicity.
AB - A DNA probe labeled with a 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) quencher and a carboxyfluorescein (FAM) donor at its 5′- and 3′-termini can be used for the detection of Hg2+ ions and phenylmercury ions (PhHg+). This DNA probe possesses a random coil structure that changes into a hairpin-like structure upon binding Hg 2+ and PhHg+ ions. As a result, the fluorescence of the FAM unit decreased through quenching between the donor and the quencher. In the presence of ethylenediaminetetraacetic acid (EDTA), the DNA probe allowed the selective detection of PhHg+ ions at concentrations as low as 70.0 nM, mainly as a result of T-Hg2+-T coordination and π-π stacking between the Ph unit and DNA bases. A linear correlation existed between the fluorescence intensity and the concentration of PhHg+ ions over the range from 0.10 to 1.0 μM (R2 = 0.99). After acid hydrolysis and neutralization of the samples, all of the mercury species are converted to Hg2+ ions, allowing us to use the DNA-based probe to determine the concentrations of total mercury species at the nM level. The practicality of this probe has been validated by the analyses of pond water and fish samples, showing its advantages of sensitivity, selectivity, and simplicity.
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U2 - 10.1039/c1an15117g
DO - 10.1039/c1an15117g
M3 - Article
VL - 136
SP - 3323
EP - 3328
JO - The Analyst
JF - The Analyst
SN - 0003-2654
IS - 16
ER -