Detection of C-reactive protein based on magnetic nanoparticles and capillary zone electrophoresis with laser-induced fluorescence detection

Yi Jyun Lin, Jian Ying Yang, Ting Yu Shu, Ting Yu Lin, Yen Yi Chen, Mei Yu Su, Wen Jie Li, Mine-Yine Liu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A simple and fast method based on magnetic nanoparticles (MNPs) and capillary zone electrophoresis (CZE) with laser-induced fluorescence (LIF) detection was developed for the detection of C-reactive protein (CRP). To optimize the CZE conditions, several factors including buffer compositions, buffer ionic strength, buffer pH, applied voltage and capillary temperature have been examined. The optimal separation buffer selected was a 30. mM sodium phosphate (PB) buffer, pH 8.0. The optimal CE applied voltage and temperature selected were 20. kV and 35. °C, respectively. The CZE profile of the MNP-1°Ab-CRP-2°Ab/FITC bioconjugates showed good reproducibility. One major peak was observed for the MNP bioconjugates. The quantitative analysis also showed good results. The coefficient of variation (CV%) for the major peak area was 8.7%, and the CV% for the major peak migration time was 2.5%. The linear range for CRP analysis was 10-150. μg/mL, and the concentration limit of detection (LOD) was 9.2. μg/mL. Non-specific interactions between bovine serum albumin (BSA) and the system can be prevented by including 10% (v/v) of human plasma in the binding buffers. The CE/LIF method might be helpful for analyzing high concentrations of CRP in a patient's plasma after an acute-phase inflammation. This new method demonstrated the possibility of using MNPs and CE/LIF for the detection of proteins, and provided information for the establishment of appropriate CE conditions.

Original languageEnglish
Pages (from-to)188-194
Number of pages7
JournalJournal of Chromatography A
Volume1315
DOIs
Publication statusPublished - 2013 Nov 8

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Capillary Electrophoresis
Electrophoresis
C-Reactive Protein
Nanoparticles
Buffers
Lasers
Fluorescence
Plasma (human)
Temperature
Fluorescein-5-isothiocyanate
Electric potential
Bovine Serum Albumin
Ionic strength
Chemical analysis
Osmolar Concentration
Limit of Detection
Inflammation
Plasmas
Proteins

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Lin, Yi Jyun ; Yang, Jian Ying ; Shu, Ting Yu ; Lin, Ting Yu ; Chen, Yen Yi ; Su, Mei Yu ; Li, Wen Jie ; Liu, Mine-Yine. / Detection of C-reactive protein based on magnetic nanoparticles and capillary zone electrophoresis with laser-induced fluorescence detection. In: Journal of Chromatography A. 2013 ; Vol. 1315. pp. 188-194.
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abstract = "A simple and fast method based on magnetic nanoparticles (MNPs) and capillary zone electrophoresis (CZE) with laser-induced fluorescence (LIF) detection was developed for the detection of C-reactive protein (CRP). To optimize the CZE conditions, several factors including buffer compositions, buffer ionic strength, buffer pH, applied voltage and capillary temperature have been examined. The optimal separation buffer selected was a 30. mM sodium phosphate (PB) buffer, pH 8.0. The optimal CE applied voltage and temperature selected were 20. kV and 35. °C, respectively. The CZE profile of the MNP-1°Ab-CRP-2°Ab/FITC bioconjugates showed good reproducibility. One major peak was observed for the MNP bioconjugates. The quantitative analysis also showed good results. The coefficient of variation (CV{\%}) for the major peak area was 8.7{\%}, and the CV{\%} for the major peak migration time was 2.5{\%}. The linear range for CRP analysis was 10-150. μg/mL, and the concentration limit of detection (LOD) was 9.2. μg/mL. Non-specific interactions between bovine serum albumin (BSA) and the system can be prevented by including 10{\%} (v/v) of human plasma in the binding buffers. The CE/LIF method might be helpful for analyzing high concentrations of CRP in a patient's plasma after an acute-phase inflammation. This new method demonstrated the possibility of using MNPs and CE/LIF for the detection of proteins, and provided information for the establishment of appropriate CE conditions.",
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Detection of C-reactive protein based on magnetic nanoparticles and capillary zone electrophoresis with laser-induced fluorescence detection. / Lin, Yi Jyun; Yang, Jian Ying; Shu, Ting Yu; Lin, Ting Yu; Chen, Yen Yi; Su, Mei Yu; Li, Wen Jie; Liu, Mine-Yine.

In: Journal of Chromatography A, Vol. 1315, 08.11.2013, p. 188-194.

Research output: Contribution to journalArticle

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T1 - Detection of C-reactive protein based on magnetic nanoparticles and capillary zone electrophoresis with laser-induced fluorescence detection

AU - Lin, Yi Jyun

AU - Yang, Jian Ying

AU - Shu, Ting Yu

AU - Lin, Ting Yu

AU - Chen, Yen Yi

AU - Su, Mei Yu

AU - Li, Wen Jie

AU - Liu, Mine-Yine

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N2 - A simple and fast method based on magnetic nanoparticles (MNPs) and capillary zone electrophoresis (CZE) with laser-induced fluorescence (LIF) detection was developed for the detection of C-reactive protein (CRP). To optimize the CZE conditions, several factors including buffer compositions, buffer ionic strength, buffer pH, applied voltage and capillary temperature have been examined. The optimal separation buffer selected was a 30. mM sodium phosphate (PB) buffer, pH 8.0. The optimal CE applied voltage and temperature selected were 20. kV and 35. °C, respectively. The CZE profile of the MNP-1°Ab-CRP-2°Ab/FITC bioconjugates showed good reproducibility. One major peak was observed for the MNP bioconjugates. The quantitative analysis also showed good results. The coefficient of variation (CV%) for the major peak area was 8.7%, and the CV% for the major peak migration time was 2.5%. The linear range for CRP analysis was 10-150. μg/mL, and the concentration limit of detection (LOD) was 9.2. μg/mL. Non-specific interactions between bovine serum albumin (BSA) and the system can be prevented by including 10% (v/v) of human plasma in the binding buffers. The CE/LIF method might be helpful for analyzing high concentrations of CRP in a patient's plasma after an acute-phase inflammation. This new method demonstrated the possibility of using MNPs and CE/LIF for the detection of proteins, and provided information for the establishment of appropriate CE conditions.

AB - A simple and fast method based on magnetic nanoparticles (MNPs) and capillary zone electrophoresis (CZE) with laser-induced fluorescence (LIF) detection was developed for the detection of C-reactive protein (CRP). To optimize the CZE conditions, several factors including buffer compositions, buffer ionic strength, buffer pH, applied voltage and capillary temperature have been examined. The optimal separation buffer selected was a 30. mM sodium phosphate (PB) buffer, pH 8.0. The optimal CE applied voltage and temperature selected were 20. kV and 35. °C, respectively. The CZE profile of the MNP-1°Ab-CRP-2°Ab/FITC bioconjugates showed good reproducibility. One major peak was observed for the MNP bioconjugates. The quantitative analysis also showed good results. The coefficient of variation (CV%) for the major peak area was 8.7%, and the CV% for the major peak migration time was 2.5%. The linear range for CRP analysis was 10-150. μg/mL, and the concentration limit of detection (LOD) was 9.2. μg/mL. Non-specific interactions between bovine serum albumin (BSA) and the system can be prevented by including 10% (v/v) of human plasma in the binding buffers. The CE/LIF method might be helpful for analyzing high concentrations of CRP in a patient's plasma after an acute-phase inflammation. This new method demonstrated the possibility of using MNPs and CE/LIF for the detection of proteins, and provided information for the establishment of appropriate CE conditions.

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