Creation of biomolecule arrays by electrostatic immobilization on electron-beam-irradiated polystyrene thin films

M. N. Wybourne, Mingdi Yan, John F.W. Keana, J. C. Wu

Research output: Contribution to journalArticle

19 Citations (Scopus)


Thin polystyrene films irradiated by an electron beam to create trapped charge are shown to immobilize the positively-charged protein, avidin-fluorescein. Using electron-beam lithography, fluorescent patterns with a minimum feature size of 0.5 μm were formed. The electrostatic nature of the immobilization was supported by the spatial and temporal features of the immobilization process. Additional evidence for an electrostatic mechanism was obtained from experiments to screen the trapped charge density with poly-L-lysine.

Original languageEnglish
Pages (from-to)302-305
Number of pages4
Issue number3
Publication statusPublished - 1996 Sep 1


All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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