Thin polystyrene films irradiated by an electron beam to create trapped charge are shown to immobilize the positively-charged protein, avidin-fluorescein. Using electron-beam lithography, fluorescent patterns with a minimum feature size of 0.5 μm were formed. The electrostatic nature of the immobilization was supported by the spatial and temporal features of the immobilization process. Additional evidence for an electrostatic mechanism was obtained from experiments to screen the trapped charge density with poly-L-lysine.
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Mechanics of Materials
- Mechanical Engineering
- Electrical and Electronic Engineering