Comparison of early transcriptome responses to copper and cadmium in rice roots

Chung Yi Lin, Ngoc Nam Trinh, Shih Feng Fu, Yu Chyuan Hsiung, Li Chiao Chia, Chung Wen Lin, Hao Jen Huang

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The phytotoxic effects of copper (Cu) and cadmium (Cd) on plant growth are well documented. However, Cu and Cd toxicity targets and the cellular systems contributing to acquisition of tolerance are not fully understood at the molecular level. We aimed to identify genes and pathways that discriminate the actions of Cu and Cd in rice roots (Oryza sativa L. cv. TN67). The transcripts of 1,450 and 1,172 genes were regulated after Cu and Cd treatments, respectively. We identified 882 genes specifically respond to Cu treatment, and 604 unique genes as Cd-responsive by comparison of expression profiles of these two regulated gene groups. Gene ontology analysis for 538 genes involved in primary metabolism, oxidation reduction and response to stimulus was changed in response to both metals. In the individual aspect, Cu specifically altered levels of genes involved in vesicle trafficking transport, fatty acid metabolism and cellular component biogenesis. Cd-regulated genes related to unfolded protein binding and sulfate assimilation. To further characterize the functions of vesicle trafficking transport under Cu stress, interference of excytosis in root tissues was conducted by inhibitors and silencing of Exo70 genes. It was demonstrated that vesicle-trafficking is required for mediation of Cu-induced reactive oxygen species (ROS) production in root tissues. These results may provide new insights into understanding the molecular basis of the early metal stress response in plants.

Original languageEnglish
Pages (from-to)507-522
Number of pages16
JournalPlant Molecular Biology
Volume81
Issue number4-5
DOIs
Publication statusPublished - 2013 Feb 12

Fingerprint

Cadmium
Transcriptome
transcriptome
Copper
cadmium
copper
rice
Genes
genes
Transport Vesicles
Metals
metals
Gene Ontology
Oryza
Gene Silencing
fatty acid metabolism
protein binding
Protein Binding
gene silencing
Sulfates

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Lin, C. Y., Trinh, N. N., Fu, S. F., Hsiung, Y. C., Chia, L. C., Lin, C. W., & Huang, H. J. (2013). Comparison of early transcriptome responses to copper and cadmium in rice roots. Plant Molecular Biology, 81(4-5), 507-522. https://doi.org/10.1007/s11103-013-0020-9
Lin, Chung Yi ; Trinh, Ngoc Nam ; Fu, Shih Feng ; Hsiung, Yu Chyuan ; Chia, Li Chiao ; Lin, Chung Wen ; Huang, Hao Jen. / Comparison of early transcriptome responses to copper and cadmium in rice roots. In: Plant Molecular Biology. 2013 ; Vol. 81, No. 4-5. pp. 507-522.
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Lin, CY, Trinh, NN, Fu, SF, Hsiung, YC, Chia, LC, Lin, CW & Huang, HJ 2013, 'Comparison of early transcriptome responses to copper and cadmium in rice roots', Plant Molecular Biology, vol. 81, no. 4-5, pp. 507-522. https://doi.org/10.1007/s11103-013-0020-9

Comparison of early transcriptome responses to copper and cadmium in rice roots. / Lin, Chung Yi; Trinh, Ngoc Nam; Fu, Shih Feng; Hsiung, Yu Chyuan; Chia, Li Chiao; Lin, Chung Wen; Huang, Hao Jen.

In: Plant Molecular Biology, Vol. 81, No. 4-5, 12.02.2013, p. 507-522.

Research output: Contribution to journalArticle

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