Chromium stress response effect on signal transduction and expression of signaling genes in rice

Ngoc Nam Trinh, Tsai Lien Huang, Wen Chang Chi, Shih-Feng Fu, Chi Chien Chen, Hao Jen Huang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Hexavalent chromium [Cr(VI)] is a non-essential metal for normal plants and is toxic to plants at high concentrations. However, signaling pathways and molecular mechanisms of its action on cell function and gene expression remain elusive. In this study, we found that Cr(VI) induced endogenous reactive oxygen species (ROS) generation and Ca2+ accumulation and activated NADPH oxidase and calcium-dependent protein kinase. We investigated global transcriptional changes in rice roots by microarray analysis. Gene expression profiling indicated activation of abscisic acid-, ethylene- and jasmonic acid-mediated signaling and inactivation of gibberellic acid-related pathways in Cr(VI) stress-treated rice roots. Genes encoding signaling components such as the protein kinases domain of unknown function 26, receptor-like cytoplasmic kinase, LRK10-like kinase type 2 and protein phosphatase 2C, as well as transcription factors WRKY and apetala2/ethylene response factor were predominant during Cr(VI) stress. Genes involved in vesicle trafficking were subjected to functional characterization. Pretreating rice roots with a vesicle trafficking inhibitor, brefeldin A, effectively reduced Cr(VI)-induced ROS production. Suppression of the vesicle trafficking gene, Exo70, by virus-induced gene silencing strategies revealed that vesicle trafficking is required for mediation of Cr(VI)-induced ROS production. Taken together, these findings shed light on the molecular mechanisms in signaling pathways and transcriptional regulation in response to Cr stress in plants.

Original languageEnglish
Pages (from-to)205-224
Number of pages20
JournalPhysiologia Plantarum
Volume150
Issue number2
DOIs
Publication statusPublished - 2014 Feb 1

Fingerprint

Chromium
chromium
signal transduction
reactive oxygen species
Signal Transduction
stress response
Gene Expression
rice
protein kinases
ethylene
phosphotransferases (kinases)
brefeldin A
calcium
gene expression
genes
gene silencing
jasmonic acid
gibberellic acid
Reactive Oxygen Species
abscisic acid

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology
  • Genetics
  • Physiology

Cite this

Trinh, Ngoc Nam ; Huang, Tsai Lien ; Chi, Wen Chang ; Fu, Shih-Feng ; Chen, Chi Chien ; Huang, Hao Jen. / Chromium stress response effect on signal transduction and expression of signaling genes in rice. In: Physiologia Plantarum. 2014 ; Vol. 150, No. 2. pp. 205-224.
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Chromium stress response effect on signal transduction and expression of signaling genes in rice. / Trinh, Ngoc Nam; Huang, Tsai Lien; Chi, Wen Chang; Fu, Shih-Feng; Chen, Chi Chien; Huang, Hao Jen.

In: Physiologia Plantarum, Vol. 150, No. 2, 01.02.2014, p. 205-224.

Research output: Contribution to journalArticle

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