Charge density profiling of circulating human low-density lipoprotein particles by capillary zone electrophoresis

Mine Yine Liu, Catherine J. McNeal, Ronald D. Macfarlane

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Capillary zone electrophoresis (CZE) has been utilized to profile the low-density (LDL) particles in human blood serum in this study. A 5 mM sodium phosphate buffer, pH 7.40, was chosen as the most suitable CE buffer and an extensive ultrafiltration (UF) procedure was applied to purify the LDL sample. Two LDL particle species, LDL with lower mobility and LDL- with higher mobility were observed. The electropherograms were highly reproducible with good precision of effective mobilities, corrected peak areas (CPAs) and CPA ratio of LDL-/LDL. LDL particles shown on the electropherogram were also characterized by several procedures. The applications of Sigma HDL cholesterol reagent and CE on-line 2-propanol precipitation indicated that the two particle species shown in the electropherogram belong to LDL. The LDL particles were found to associate with the buoyant LDL fraction and the LDL- particles associate with the dense LDLfraction. This study utilizes CZE forthe profiling of LDL isoforms and provides a new analytical method for the resolution of LDL subspecies. It demonstrates a high-mobility LDL particle which circulates in healthy subjects and diminishes in atherosclerotic patients. Diminution of the high-mobility LDL subspecies may be linked to minimal formation of arterial plaque in atherosclerotic patients.

Original languageEnglish
Pages (from-to)2985-2995
Number of pages11
JournalElectrophoresis
Volume25
Issue number17
DOIs
Publication statusPublished - 2004 Sep 1

Fingerprint

Capillary Electrophoresis
Charge density
Electrophoresis
LDL Lipoproteins
oxidized low density lipoprotein
Buffers
2-Propanol
Ultrafiltration
Atherosclerotic Plaques
HDL Cholesterol
Healthy Volunteers
Protein Isoforms

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Liu, Mine Yine ; McNeal, Catherine J. ; Macfarlane, Ronald D. / Charge density profiling of circulating human low-density lipoprotein particles by capillary zone electrophoresis. In: Electrophoresis. 2004 ; Vol. 25, No. 17. pp. 2985-2995.
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Charge density profiling of circulating human low-density lipoprotein particles by capillary zone electrophoresis. / Liu, Mine Yine; McNeal, Catherine J.; Macfarlane, Ronald D.

In: Electrophoresis, Vol. 25, No. 17, 01.09.2004, p. 2985-2995.

Research output: Contribution to journalArticle

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