Characterization of in vitro modified human very low-density Lipoprotein particles and phospholipids by capillary electrophoresis

Yi Ning Liu, Ting Yu Shu, Huai Guang Xie, Wei Ting Lai, Yi Han Liao, Mei Yu Su, You Sian Lin, Yen Yi Chen, Yi Jyun Lin, Chin Pong Chong, Mine Yine Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple capillary zone electrophoresis (CZE) method was used to characterize human very low-density lipoprotein (VLDL) particles for four healthy donors. One major peak was observed for native, in vitro oxidized and glycated VLDL particles. The effective mobilities and peak areas of the capillary electrophoresis (CE) profiles showed good reproducibility and precision. The mobility of the oxidized VLDL peak was higher than that of the native VLDL. The mobility of the glycated VLDL peak was similar to that of the native VLDL. Phospholipids isolated from VLDL particles were analyzed by our recently developed micellar electrokinetic chromatography (MEKC) with a high-salt stacking method. At absorbance 200 nm, the native VLDL phospholipids showed a major peak and a minor peak for each donor. For oxidized VLDL phospholipids, the area of the major peak reduced for three donors, possibly due to phospholipid decomposition. For glycated VLDL phospholipids, the peak mobilities were more positive than native VLDL phospholipids for two donors, possibly due to phospholipid-linked advanced glycation end products (AGEs). Very interestingly, at absorbance 234 nm, the major peak of oxidized VLDL phospholipids was resolved as two peaks for each donor, possibly due to conjugated dienes formed upon oxidation.

Original languageEnglish
Pages (from-to)16400-16417
Number of pages18
JournalInternational Journal of Molecular Sciences
Volume13
Issue number12
DOIs
Publication statusPublished - 2012 Dec

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this