Characterization of in vitro modified human very low-density Lipoprotein particles and phospholipids by capillary electrophoresis

Yi Ning Liu, Ting Yu Shu, Huai Guang Xie, Wei Ting Lai, Yi Han Liao, Mei Yu Su, You Sian Lin, Yen Yi Chen, Yi Jyun Lin, Chin Pong Chong, Mine Yine Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple capillary zone electrophoresis (CZE) method was used to characterize human very low-density lipoprotein (VLDL) particles for four healthy donors. One major peak was observed for native, in vitro oxidized and glycated VLDL particles. The effective mobilities and peak areas of the capillary electrophoresis (CE) profiles showed good reproducibility and precision. The mobility of the oxidized VLDL peak was higher than that of the native VLDL. The mobility of the glycated VLDL peak was similar to that of the native VLDL. Phospholipids isolated from VLDL particles were analyzed by our recently developed micellar electrokinetic chromatography (MEKC) with a high-salt stacking method. At absorbance 200 nm, the native VLDL phospholipids showed a major peak and a minor peak for each donor. For oxidized VLDL phospholipids, the area of the major peak reduced for three donors, possibly due to phospholipid decomposition. For glycated VLDL phospholipids, the peak mobilities were more positive than native VLDL phospholipids for two donors, possibly due to phospholipid-linked advanced glycation end products (AGEs). Very interestingly, at absorbance 234 nm, the major peak of oxidized VLDL phospholipids was resolved as two peaks for each donor, possibly due to conjugated dienes formed upon oxidation.

Original languageEnglish
Pages (from-to)16400-16417
Number of pages18
JournalInternational Journal of Molecular Sciences
Volume13
Issue number12
DOIs
Publication statusPublished - 2012 Dec

Fingerprint

lipoproteins
Lipoproteins
Capillary electrophoresis
VLDL Lipoproteins
Phospholipids
Capillary Electrophoresis
electrophoresis
Advanced Glycosylation End Products
In Vitro Techniques
Chromatography
Electrophoresis
Salts
electrokinetics
dienes
chromatography
Decomposition
oxidized very low density lipoprotein
Oxidation

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Liu, Yi Ning ; Shu, Ting Yu ; Xie, Huai Guang ; Lai, Wei Ting ; Liao, Yi Han ; Su, Mei Yu ; Lin, You Sian ; Chen, Yen Yi ; Lin, Yi Jyun ; Chong, Chin Pong ; Liu, Mine Yine. / Characterization of in vitro modified human very low-density Lipoprotein particles and phospholipids by capillary electrophoresis. In: International Journal of Molecular Sciences. 2012 ; Vol. 13, No. 12. pp. 16400-16417.
@article{8ec0ff6341f846249d6e85bf3b6cf0ea,
title = "Characterization of in vitro modified human very low-density Lipoprotein particles and phospholipids by capillary electrophoresis",
abstract = "A simple capillary zone electrophoresis (CZE) method was used to characterize human very low-density lipoprotein (VLDL) particles for four healthy donors. One major peak was observed for native, in vitro oxidized and glycated VLDL particles. The effective mobilities and peak areas of the capillary electrophoresis (CE) profiles showed good reproducibility and precision. The mobility of the oxidized VLDL peak was higher than that of the native VLDL. The mobility of the glycated VLDL peak was similar to that of the native VLDL. Phospholipids isolated from VLDL particles were analyzed by our recently developed micellar electrokinetic chromatography (MEKC) with a high-salt stacking method. At absorbance 200 nm, the native VLDL phospholipids showed a major peak and a minor peak for each donor. For oxidized VLDL phospholipids, the area of the major peak reduced for three donors, possibly due to phospholipid decomposition. For glycated VLDL phospholipids, the peak mobilities were more positive than native VLDL phospholipids for two donors, possibly due to phospholipid-linked advanced glycation end products (AGEs). Very interestingly, at absorbance 234 nm, the major peak of oxidized VLDL phospholipids was resolved as two peaks for each donor, possibly due to conjugated dienes formed upon oxidation.",
author = "Liu, {Yi Ning} and Shu, {Ting Yu} and Xie, {Huai Guang} and Lai, {Wei Ting} and Liao, {Yi Han} and Su, {Mei Yu} and Lin, {You Sian} and Chen, {Yen Yi} and Lin, {Yi Jyun} and Chong, {Chin Pong} and Liu, {Mine Yine}",
year = "2012",
month = "12",
doi = "10.3390/ijms131216400",
language = "English",
volume = "13",
pages = "16400--16417",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",

}

Characterization of in vitro modified human very low-density Lipoprotein particles and phospholipids by capillary electrophoresis. / Liu, Yi Ning; Shu, Ting Yu; Xie, Huai Guang; Lai, Wei Ting; Liao, Yi Han; Su, Mei Yu; Lin, You Sian; Chen, Yen Yi; Lin, Yi Jyun; Chong, Chin Pong; Liu, Mine Yine.

In: International Journal of Molecular Sciences, Vol. 13, No. 12, 12.2012, p. 16400-16417.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of in vitro modified human very low-density Lipoprotein particles and phospholipids by capillary electrophoresis

AU - Liu, Yi Ning

AU - Shu, Ting Yu

AU - Xie, Huai Guang

AU - Lai, Wei Ting

AU - Liao, Yi Han

AU - Su, Mei Yu

AU - Lin, You Sian

AU - Chen, Yen Yi

AU - Lin, Yi Jyun

AU - Chong, Chin Pong

AU - Liu, Mine Yine

PY - 2012/12

Y1 - 2012/12

N2 - A simple capillary zone electrophoresis (CZE) method was used to characterize human very low-density lipoprotein (VLDL) particles for four healthy donors. One major peak was observed for native, in vitro oxidized and glycated VLDL particles. The effective mobilities and peak areas of the capillary electrophoresis (CE) profiles showed good reproducibility and precision. The mobility of the oxidized VLDL peak was higher than that of the native VLDL. The mobility of the glycated VLDL peak was similar to that of the native VLDL. Phospholipids isolated from VLDL particles were analyzed by our recently developed micellar electrokinetic chromatography (MEKC) with a high-salt stacking method. At absorbance 200 nm, the native VLDL phospholipids showed a major peak and a minor peak for each donor. For oxidized VLDL phospholipids, the area of the major peak reduced for three donors, possibly due to phospholipid decomposition. For glycated VLDL phospholipids, the peak mobilities were more positive than native VLDL phospholipids for two donors, possibly due to phospholipid-linked advanced glycation end products (AGEs). Very interestingly, at absorbance 234 nm, the major peak of oxidized VLDL phospholipids was resolved as two peaks for each donor, possibly due to conjugated dienes formed upon oxidation.

AB - A simple capillary zone electrophoresis (CZE) method was used to characterize human very low-density lipoprotein (VLDL) particles for four healthy donors. One major peak was observed for native, in vitro oxidized and glycated VLDL particles. The effective mobilities and peak areas of the capillary electrophoresis (CE) profiles showed good reproducibility and precision. The mobility of the oxidized VLDL peak was higher than that of the native VLDL. The mobility of the glycated VLDL peak was similar to that of the native VLDL. Phospholipids isolated from VLDL particles were analyzed by our recently developed micellar electrokinetic chromatography (MEKC) with a high-salt stacking method. At absorbance 200 nm, the native VLDL phospholipids showed a major peak and a minor peak for each donor. For oxidized VLDL phospholipids, the area of the major peak reduced for three donors, possibly due to phospholipid decomposition. For glycated VLDL phospholipids, the peak mobilities were more positive than native VLDL phospholipids for two donors, possibly due to phospholipid-linked advanced glycation end products (AGEs). Very interestingly, at absorbance 234 nm, the major peak of oxidized VLDL phospholipids was resolved as two peaks for each donor, possibly due to conjugated dienes formed upon oxidation.

UR - http://www.scopus.com/inward/record.url?scp=84871678380&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871678380&partnerID=8YFLogxK

U2 - 10.3390/ijms131216400

DO - 10.3390/ijms131216400

M3 - Article

C2 - 23208377

AN - SCOPUS:84871678380

VL - 13

SP - 16400

EP - 16417

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 12

ER -