Characterization of in vitro modified human high-density lipoprotein particles and phospholipids by capillary zone electrophoresis and LC ESI-MS

Chung Yu Wu, Yu Nong Peng, Jing Huei Chiu, Yu Ling Ho, Chin Pong Chong, Ying Ling Yang, Mine-Yine Liu

Research output: Contribution to journalArticle

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Abstract

A simple capillary zone electrophoresis (CZE) method was used to characterize native, in vitro oxidized and glycated human high-density lipoprotein (HDL) particles. Both native and in vitro oxidized HDL capillary electrophoresis (CE) profiles showed a major peak, but the oxidized HDL particles had higher effective mobilities. The in vitro glycated HDL particles showed a major peak and one or two minor peaks. The effective mobility of the major peak of glycated HDL was similar to that of the major peak of native HDL, whereas the effective mobilities of the two minor peaks were much lower. For the analysis of HDL phospholipids, a solid phase extraction procedure was optimized and a LC ESI-MS method was developed. Several possible HDL phospholipid molecular species including phosphatidylcholine (PC 16:0/18:2, 16:0/18:1, 18:0/18:2 and 18:0/18:1), sphingomyelin (SM 16:0) and lyso-phosphatidylcholine (lysoPC 16:0 and 18:0) were found. It appeared that the ion intensity ratios of hydroperoxy-PC or epoxyhydroxy-PC (16:0/hydroperoxy-18:2 or 16:0/epoxyhydroxy-18:2, m/z 790.4) and trihydroxy-PC (16:0/trihydroxy-18:2, m/z 808.3) relative to PC (C16:0/C18:2, m/z 758.5) were higher for oxidized HDL than for native and glycated HDL. It should be helpful to use both CZE and LC ESI-MS methods for analyzing high-density lipoproteins from patients of cardiovascular disease. Their combination may be also useful for further studies concerning the role of oxidized and glycated HDLs in the development of atherosclerosis.

Original languageEnglish
Pages (from-to)3495-3505
Number of pages11
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number29
DOIs
Publication statusPublished - 2009 Nov 1

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Capillary Electrophoresis
HDL Lipoproteins
Electrophoresis
Phospholipids
Phosphatidylcholines
In Vitro Techniques
Capillary electrophoresis
Sphingomyelins
Solid Phase Extraction
Atherosclerosis
Cardiovascular Diseases
Ions

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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title = "Characterization of in vitro modified human high-density lipoprotein particles and phospholipids by capillary zone electrophoresis and LC ESI-MS",
abstract = "A simple capillary zone electrophoresis (CZE) method was used to characterize native, in vitro oxidized and glycated human high-density lipoprotein (HDL) particles. Both native and in vitro oxidized HDL capillary electrophoresis (CE) profiles showed a major peak, but the oxidized HDL particles had higher effective mobilities. The in vitro glycated HDL particles showed a major peak and one or two minor peaks. The effective mobility of the major peak of glycated HDL was similar to that of the major peak of native HDL, whereas the effective mobilities of the two minor peaks were much lower. For the analysis of HDL phospholipids, a solid phase extraction procedure was optimized and a LC ESI-MS method was developed. Several possible HDL phospholipid molecular species including phosphatidylcholine (PC 16:0/18:2, 16:0/18:1, 18:0/18:2 and 18:0/18:1), sphingomyelin (SM 16:0) and lyso-phosphatidylcholine (lysoPC 16:0 and 18:0) were found. It appeared that the ion intensity ratios of hydroperoxy-PC or epoxyhydroxy-PC (16:0/hydroperoxy-18:2 or 16:0/epoxyhydroxy-18:2, m/z 790.4) and trihydroxy-PC (16:0/trihydroxy-18:2, m/z 808.3) relative to PC (C16:0/C18:2, m/z 758.5) were higher for oxidized HDL than for native and glycated HDL. It should be helpful to use both CZE and LC ESI-MS methods for analyzing high-density lipoproteins from patients of cardiovascular disease. Their combination may be also useful for further studies concerning the role of oxidized and glycated HDLs in the development of atherosclerosis.",
author = "Wu, {Chung Yu} and Peng, {Yu Nong} and Chiu, {Jing Huei} and Ho, {Yu Ling} and Chong, {Chin Pong} and Yang, {Ying Ling} and Mine-Yine Liu",
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Characterization of in vitro modified human high-density lipoprotein particles and phospholipids by capillary zone electrophoresis and LC ESI-MS. / Wu, Chung Yu; Peng, Yu Nong; Chiu, Jing Huei; Ho, Yu Ling; Chong, Chin Pong; Yang, Ying Ling; Liu, Mine-Yine.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 29, 01.11.2009, p. 3495-3505.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of in vitro modified human high-density lipoprotein particles and phospholipids by capillary zone electrophoresis and LC ESI-MS

AU - Wu, Chung Yu

AU - Peng, Yu Nong

AU - Chiu, Jing Huei

AU - Ho, Yu Ling

AU - Chong, Chin Pong

AU - Yang, Ying Ling

AU - Liu, Mine-Yine

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N2 - A simple capillary zone electrophoresis (CZE) method was used to characterize native, in vitro oxidized and glycated human high-density lipoprotein (HDL) particles. Both native and in vitro oxidized HDL capillary electrophoresis (CE) profiles showed a major peak, but the oxidized HDL particles had higher effective mobilities. The in vitro glycated HDL particles showed a major peak and one or two minor peaks. The effective mobility of the major peak of glycated HDL was similar to that of the major peak of native HDL, whereas the effective mobilities of the two minor peaks were much lower. For the analysis of HDL phospholipids, a solid phase extraction procedure was optimized and a LC ESI-MS method was developed. Several possible HDL phospholipid molecular species including phosphatidylcholine (PC 16:0/18:2, 16:0/18:1, 18:0/18:2 and 18:0/18:1), sphingomyelin (SM 16:0) and lyso-phosphatidylcholine (lysoPC 16:0 and 18:0) were found. It appeared that the ion intensity ratios of hydroperoxy-PC or epoxyhydroxy-PC (16:0/hydroperoxy-18:2 or 16:0/epoxyhydroxy-18:2, m/z 790.4) and trihydroxy-PC (16:0/trihydroxy-18:2, m/z 808.3) relative to PC (C16:0/C18:2, m/z 758.5) were higher for oxidized HDL than for native and glycated HDL. It should be helpful to use both CZE and LC ESI-MS methods for analyzing high-density lipoproteins from patients of cardiovascular disease. Their combination may be also useful for further studies concerning the role of oxidized and glycated HDLs in the development of atherosclerosis.

AB - A simple capillary zone electrophoresis (CZE) method was used to characterize native, in vitro oxidized and glycated human high-density lipoprotein (HDL) particles. Both native and in vitro oxidized HDL capillary electrophoresis (CE) profiles showed a major peak, but the oxidized HDL particles had higher effective mobilities. The in vitro glycated HDL particles showed a major peak and one or two minor peaks. The effective mobility of the major peak of glycated HDL was similar to that of the major peak of native HDL, whereas the effective mobilities of the two minor peaks were much lower. For the analysis of HDL phospholipids, a solid phase extraction procedure was optimized and a LC ESI-MS method was developed. Several possible HDL phospholipid molecular species including phosphatidylcholine (PC 16:0/18:2, 16:0/18:1, 18:0/18:2 and 18:0/18:1), sphingomyelin (SM 16:0) and lyso-phosphatidylcholine (lysoPC 16:0 and 18:0) were found. It appeared that the ion intensity ratios of hydroperoxy-PC or epoxyhydroxy-PC (16:0/hydroperoxy-18:2 or 16:0/epoxyhydroxy-18:2, m/z 790.4) and trihydroxy-PC (16:0/trihydroxy-18:2, m/z 808.3) relative to PC (C16:0/C18:2, m/z 758.5) were higher for oxidized HDL than for native and glycated HDL. It should be helpful to use both CZE and LC ESI-MS methods for analyzing high-density lipoproteins from patients of cardiovascular disease. Their combination may be also useful for further studies concerning the role of oxidized and glycated HDLs in the development of atherosclerosis.

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