Characterization of fish Cu/Zn-superoxide dismutase and its protection from oxidative stress

Chuian-Fu Ken, Chi Tsai Lin, Jei Fu Shaw, Jen Leih Wu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Copper/zinc superoxide dismutase was cloned from the zebrafish (Danio rerio). The full coding region of the zebrafish superoxide dismutase (ZSOD) complementary DNA was ligated with pET-20b(+) and successfully expressed in Escherichia coli strain AD494(DE3)pLysS. The active enzyme was purified by His tagging. The ZSOD yield was 6 mg from 0.2 L of E. coli culture, and the specific activity was 2000 U/mg as assayed using a RANSOD kit. The enzyme stability was characterized by reaction to temperature, pH, and detergent treatment. The results showed enzyme activity was still active after heat treatment at 70°C for 10 minutes, resistant to pH treatment from 2.3 to 12, and resistant to treatment with sodium dodecyl sulfate (SDS) under 4%. In addition, the recombinant ZSOD was used to protect fish from 100 ppm of paraquat-induced oxidative injury, by soaking fish larva in 55 μg/ml SOD enzyme. The results were significant.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalMarine Biotechnology
Volume5
Issue number2
DOIs
Publication statusPublished - 2003 Mar 1

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Zebrafish
Danio rerio
superoxide dismutase
Fishes
Oxidative Stress
oxidative stress
Superoxide Dismutase
enzyme
fish
paraquat
tagging
detergent
Enzymes
enzyme activity
Escherichia coli
enzyme stability
Enzyme Stability
zinc
complementary DNA
sodium

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Biotechnology

Cite this

Ken, Chuian-Fu ; Lin, Chi Tsai ; Shaw, Jei Fu ; Wu, Jen Leih. / Characterization of fish Cu/Zn-superoxide dismutase and its protection from oxidative stress. In: Marine Biotechnology. 2003 ; Vol. 5, No. 2. pp. 167-173.
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Characterization of fish Cu/Zn-superoxide dismutase and its protection from oxidative stress. / Ken, Chuian-Fu; Lin, Chi Tsai; Shaw, Jei Fu; Wu, Jen Leih.

In: Marine Biotechnology, Vol. 5, No. 2, 01.03.2003, p. 167-173.

Research output: Contribution to journalArticle

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