Abstract
A 977 bp cDNA containing an open reading frame encoding 224 amino acid residues of manganese superoxide dismutase was cloned from zebrafish (zMn-SOD). The deduced amino acid sequence showed high identity with the sequences of Mn-SODs from human (85.1%) to nematode (61.6%). The 3-D structure model was superimposed on the relative domains of human Mn-SOD with the root mean square (rms) deviation of 0.0919 Å. The recombinant mature zMn-SOD with enzyme activity was purified using His-tag technique. The half-life of the enzyme is approximately 48 min and its thermal inactivation rate constant kd is 0.0154 min-1at 70 °C. The enzyme was active under a broad pH (2.2-11.2) and in the presence of up to 4% SDS. Real-time RT-PCR assay was used to detect the zMn-SOD mRNA expression during the developmental stages following a challenge with paraquat. A high level expression of Mn-SOD mRNA was detected at the cleavage stage, but decreased significantly under paraquat treatment. The results indicated that Mn-SOD plays an important role during embryonic development.
| Original language | English |
|---|---|
| Pages (from-to) | 318-324 |
| Number of pages | 7 |
| Journal | Fish and Shellfish Immunology |
| Volume | 27 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Aug |
Fingerprint
All Science Journal Classification (ASJC) codes
- Environmental Chemistry
- Aquatic Science
Cite this
}
Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase. / Lin, Chi Tsai; Tseng, Wen Chung; Hsiao, Nai Wan; Chang, Hsiao Huang; Ken, Chuian Fu.
In: Fish and Shellfish Immunology, Vol. 27, No. 2, 08.2009, p. 318-324.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase
AU - Lin, Chi Tsai
AU - Tseng, Wen Chung
AU - Hsiao, Nai Wan
AU - Chang, Hsiao Huang
AU - Ken, Chuian Fu
PY - 2009/8
Y1 - 2009/8
N2 - A 977 bp cDNA containing an open reading frame encoding 224 amino acid residues of manganese superoxide dismutase was cloned from zebrafish (zMn-SOD). The deduced amino acid sequence showed high identity with the sequences of Mn-SODs from human (85.1%) to nematode (61.6%). The 3-D structure model was superimposed on the relative domains of human Mn-SOD with the root mean square (rms) deviation of 0.0919 Å. The recombinant mature zMn-SOD with enzyme activity was purified using His-tag technique. The half-life of the enzyme is approximately 48 min and its thermal inactivation rate constant kd is 0.0154 min-1at 70 °C. The enzyme was active under a broad pH (2.2-11.2) and in the presence of up to 4% SDS. Real-time RT-PCR assay was used to detect the zMn-SOD mRNA expression during the developmental stages following a challenge with paraquat. A high level expression of Mn-SOD mRNA was detected at the cleavage stage, but decreased significantly under paraquat treatment. The results indicated that Mn-SOD plays an important role during embryonic development.
AB - A 977 bp cDNA containing an open reading frame encoding 224 amino acid residues of manganese superoxide dismutase was cloned from zebrafish (zMn-SOD). The deduced amino acid sequence showed high identity with the sequences of Mn-SODs from human (85.1%) to nematode (61.6%). The 3-D structure model was superimposed on the relative domains of human Mn-SOD with the root mean square (rms) deviation of 0.0919 Å. The recombinant mature zMn-SOD with enzyme activity was purified using His-tag technique. The half-life of the enzyme is approximately 48 min and its thermal inactivation rate constant kd is 0.0154 min-1at 70 °C. The enzyme was active under a broad pH (2.2-11.2) and in the presence of up to 4% SDS. Real-time RT-PCR assay was used to detect the zMn-SOD mRNA expression during the developmental stages following a challenge with paraquat. A high level expression of Mn-SOD mRNA was detected at the cleavage stage, but decreased significantly under paraquat treatment. The results indicated that Mn-SOD plays an important role during embryonic development.
UR - http://www.scopus.com/inward/record.url?scp=67650477815&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650477815&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2009.05.015
DO - 10.1016/j.fsi.2009.05.015
M3 - Article
C2 - 19501168
AN - SCOPUS:67650477815
VL - 27
SP - 318
EP - 324
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
SN - 1050-4648
IS - 2
ER -