Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase

Chi Tsai Lin, Wen Chung Tseng, Nai Wan Hsiao, Hsiao Huang Chang, Chuian Fu Ken

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A 977 bp cDNA containing an open reading frame encoding 224 amino acid residues of manganese superoxide dismutase was cloned from zebrafish (zMn-SOD). The deduced amino acid sequence showed high identity with the sequences of Mn-SODs from human (85.1%) to nematode (61.6%). The 3-D structure model was superimposed on the relative domains of human Mn-SOD with the root mean square (rms) deviation of 0.0919 Å. The recombinant mature zMn-SOD with enzyme activity was purified using His-tag technique. The half-life of the enzyme is approximately 48 min and its thermal inactivation rate constant kd is 0.0154 min-1at 70 °C. The enzyme was active under a broad pH (2.2-11.2) and in the presence of up to 4% SDS. Real-time RT-PCR assay was used to detect the zMn-SOD mRNA expression during the developmental stages following a challenge with paraquat. A high level expression of Mn-SOD mRNA was detected at the cleavage stage, but decreased significantly under paraquat treatment. The results indicated that Mn-SOD plays an important role during embryonic development.

Original languageEnglish
Pages (from-to)318-324
Number of pages7
JournalFish and Shellfish Immunology
Volume27
Issue number2
DOIs
Publication statusPublished - 2009 Aug

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Fingerprint Dive into the research topics of 'Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase'. Together they form a unique fingerprint.

  • Cite this