Cell flow analysis with a two-photon fluorescence fiber probe

Yu Chung Chang, Jing Yong Ye, Thommey P. Thomas, James R. Baker, Theodore B. Norris

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


We report the use of a sensitive double-clad fiber (DCF) probe for in situ cell flow velocity measurements and cell analysis by means of two-photon excited fluorescence correlation spectroscopy (FCS). We have demonstrated the feasibility to use this fiber probe for in vivo two-photon flow cytometry previously. However, because of the viscosity of blood and the non-uniform flow nature in vivo, it is problematic to use the detected cell numbers to estimate the sampled blood volume. To precisely calibrate the sampled blood volume, it is necessary to conduct real time flow velocity measurement. We propose to use FCS technique to measure the flow velocity. The ability to measure the flow velocities of labeled cells in whole blood has been demonstrated. Our two-photon fluorescence fiber probe has the ability to monitor multiple fluorescent biomarkers simultaneously. We demonstrate that we can distinguish differently labeled cells by their distinct features on the correlation curves. The ability to conduct in situ cell flow analysis using the fiber probe may be useful in disease diagnosis or further comprehension of the circulation system.

Original languageEnglish
Title of host publicationOptics in Health Care and Biomedical Optics IV
Publication statusPublished - 2010 Dec 29
EventOptics in Health Care and Biomedical Optics IV - Beijing, China
Duration: 2010 Oct 182010 Oct 20

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherOptics in Health Care and Biomedical Optics IV

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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