Abstract
Fluorescence is popular in carbohydrate, protein, and DNA arrays; cell imaging; and single molecule detection because of its sensitivity. To achieve high sensitivity, fluorophores possessing high quantum yields are necessary. However, most analytes do not fluoresce strongly. In order to overcome this shortage, techniques based on fluorescence quenching and surface-enhanced fluorescence using novel metal materials have been applied. Fluorescence quenching and enhancements are both dependent on the distance of fluorophores from the metal surface; quenching usually occurs at a distance no greater than 50 Å while fluorescence enhancement commonly takes place at a distance between 70 and 100 Å. In addition to distance, the nature, size, and shape of metal materials are important parameters for fluorescence quenching and enhancement. With advanced nanotechnology, researchers have demonstrated techniques entailing fluorescence quenching and enhancement using novel metal nanomaterials for the sensitive analysis of proteins, DNA, and small solutes.
| Original language | English |
|---|---|
| Title of host publication | From Bioimaging to Biosensors |
| Subtitle of host publication | Noble Metal Nanoparticles in Biodetection |
| Publisher | Pan Stanford Publishing Pte. Ltd. |
| Pages | 211-248 |
| Number of pages | 38 |
| ISBN (Electronic) | 9789814303101 |
| ISBN (Print) | 9789814267243 |
| Publication status | Published - 2012 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Medicine(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Chemistry(all)
- Engineering(all)
- Materials Science(all)
Cite this
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Biodetection based on fluorescence quenching and surface-enhanced fluorescence using noble metal nanoparticles. / Lin, Yang-Wei; Liu, Chi Wei; Chang, Huan Tsung.
From Bioimaging to Biosensors: Noble Metal Nanoparticles in Biodetection. Pan Stanford Publishing Pte. Ltd., 2012. p. 211-248.Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Biodetection based on fluorescence quenching and surface-enhanced fluorescence using noble metal nanoparticles
AU - Lin, Yang-Wei
AU - Liu, Chi Wei
AU - Chang, Huan Tsung
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Fluorescence is popular in carbohydrate, protein, and DNA arrays; cell imaging; and single molecule detection because of its sensitivity. To achieve high sensitivity, fluorophores possessing high quantum yields are necessary. However, most analytes do not fluoresce strongly. In order to overcome this shortage, techniques based on fluorescence quenching and surface-enhanced fluorescence using novel metal materials have been applied. Fluorescence quenching and enhancements are both dependent on the distance of fluorophores from the metal surface; quenching usually occurs at a distance no greater than 50 Å while fluorescence enhancement commonly takes place at a distance between 70 and 100 Å. In addition to distance, the nature, size, and shape of metal materials are important parameters for fluorescence quenching and enhancement. With advanced nanotechnology, researchers have demonstrated techniques entailing fluorescence quenching and enhancement using novel metal nanomaterials for the sensitive analysis of proteins, DNA, and small solutes.
AB - Fluorescence is popular in carbohydrate, protein, and DNA arrays; cell imaging; and single molecule detection because of its sensitivity. To achieve high sensitivity, fluorophores possessing high quantum yields are necessary. However, most analytes do not fluoresce strongly. In order to overcome this shortage, techniques based on fluorescence quenching and surface-enhanced fluorescence using novel metal materials have been applied. Fluorescence quenching and enhancements are both dependent on the distance of fluorophores from the metal surface; quenching usually occurs at a distance no greater than 50 Å while fluorescence enhancement commonly takes place at a distance between 70 and 100 Å. In addition to distance, the nature, size, and shape of metal materials are important parameters for fluorescence quenching and enhancement. With advanced nanotechnology, researchers have demonstrated techniques entailing fluorescence quenching and enhancement using novel metal nanomaterials for the sensitive analysis of proteins, DNA, and small solutes.
UR - http://www.scopus.com/inward/record.url?scp=84886100426&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886100426&partnerID=8YFLogxK
M3 - Chapter
AN - SCOPUS:84886100426
SN - 9789814267243
SP - 211
EP - 248
BT - From Bioimaging to Biosensors
PB - Pan Stanford Publishing Pte. Ltd.
ER -