Abstract
Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. A gene encoding a novel 2-Cys Prx was identified based on sequence homology in an expressed sequence tag database of the Antrodia camphorata, a medicinal mushroom found only in Taiwan. The 2-Cys Prx cDNA (940 bp) encodes a protein of 188 amino acid residues with calculated molecular mass of 20,965 Da and a pI of 5.89. The coding region was subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein. The purified enzyme was characterized under various conditions. The Prx retained 68% activity after being heated at 60°C for 2 min. It was stable under a broad pH range from 5 to 11. The enzyme activity was slightly decreased in the presence of 1% sodium dodecyl sulfate. The enzyme was somewhat susceptible to chymotrypsin treatment but resistant to digestion by trypsin.
| Original language | English |
|---|---|
| Pages (from-to) | 84-92 |
| Number of pages | 9 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 74 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2007 Feb 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
}
Biochemical characterization of a novel 2-Cys peroxiredoxin from Antrodia camphorata. / Huang, Jenq Kuen; Ken, Chuian-Fu; Huang, Hui Ming; Lin, Chi Tsai.
In: Applied Microbiology and Biotechnology, Vol. 74, No. 1, 01.02.2007, p. 84-92.Research output: Contribution to journal › Article
TY - JOUR
T1 - Biochemical characterization of a novel 2-Cys peroxiredoxin from Antrodia camphorata
AU - Huang, Jenq Kuen
AU - Ken, Chuian-Fu
AU - Huang, Hui Ming
AU - Lin, Chi Tsai
PY - 2007/2/1
Y1 - 2007/2/1
N2 - Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. A gene encoding a novel 2-Cys Prx was identified based on sequence homology in an expressed sequence tag database of the Antrodia camphorata, a medicinal mushroom found only in Taiwan. The 2-Cys Prx cDNA (940 bp) encodes a protein of 188 amino acid residues with calculated molecular mass of 20,965 Da and a pI of 5.89. The coding region was subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein. The purified enzyme was characterized under various conditions. The Prx retained 68% activity after being heated at 60°C for 2 min. It was stable under a broad pH range from 5 to 11. The enzyme activity was slightly decreased in the presence of 1% sodium dodecyl sulfate. The enzyme was somewhat susceptible to chymotrypsin treatment but resistant to digestion by trypsin.
AB - Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. A gene encoding a novel 2-Cys Prx was identified based on sequence homology in an expressed sequence tag database of the Antrodia camphorata, a medicinal mushroom found only in Taiwan. The 2-Cys Prx cDNA (940 bp) encodes a protein of 188 amino acid residues with calculated molecular mass of 20,965 Da and a pI of 5.89. The coding region was subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein. The purified enzyme was characterized under various conditions. The Prx retained 68% activity after being heated at 60°C for 2 min. It was stable under a broad pH range from 5 to 11. The enzyme activity was slightly decreased in the presence of 1% sodium dodecyl sulfate. The enzyme was somewhat susceptible to chymotrypsin treatment but resistant to digestion by trypsin.
UR - http://www.scopus.com/inward/record.url?scp=33846641609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846641609&partnerID=8YFLogxK
U2 - 10.1007/s00253-006-0626-9
DO - 10.1007/s00253-006-0626-9
M3 - Article
C2 - 17031636
AN - SCOPUS:33846641609
VL - 74
SP - 84
EP - 92
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 1
ER -