Biochemical characterization of a novel 2-Cys peroxiredoxin from Antrodia camphorata

Jenq Kuen Huang, Chuian-Fu Ken, Hui Ming Huang, Chi Tsai Lin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. A gene encoding a novel 2-Cys Prx was identified based on sequence homology in an expressed sequence tag database of the Antrodia camphorata, a medicinal mushroom found only in Taiwan. The 2-Cys Prx cDNA (940 bp) encodes a protein of 188 amino acid residues with calculated molecular mass of 20,965 Da and a pI of 5.89. The coding region was subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein. The purified enzyme was characterized under various conditions. The Prx retained 68% activity after being heated at 60°C for 2 min. It was stable under a broad pH range from 5 to 11. The enzyme activity was slightly decreased in the presence of 1% sodium dodecyl sulfate. The enzyme was somewhat susceptible to chymotrypsin treatment but resistant to digestion by trypsin.

Original languageEnglish
Pages (from-to)84-92
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume74
Issue number1
DOIs
Publication statusPublished - 2007 Feb 1

Fingerprint

Antrodia
Peroxiredoxins
Enzymes
Agaricales
Expressed Sequence Tags
Chymotrypsin
Sequence Homology
Taiwan
Sodium Dodecyl Sulfate
Trypsin
Digestion
Proteins
Complementary DNA
Databases
Escherichia coli
Amino Acids
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Huang, Jenq Kuen ; Ken, Chuian-Fu ; Huang, Hui Ming ; Lin, Chi Tsai. / Biochemical characterization of a novel 2-Cys peroxiredoxin from Antrodia camphorata. In: Applied Microbiology and Biotechnology. 2007 ; Vol. 74, No. 1. pp. 84-92.
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abstract = "Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. A gene encoding a novel 2-Cys Prx was identified based on sequence homology in an expressed sequence tag database of the Antrodia camphorata, a medicinal mushroom found only in Taiwan. The 2-Cys Prx cDNA (940 bp) encodes a protein of 188 amino acid residues with calculated molecular mass of 20,965 Da and a pI of 5.89. The coding region was subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein. The purified enzyme was characterized under various conditions. The Prx retained 68{\%} activity after being heated at 60°C for 2 min. It was stable under a broad pH range from 5 to 11. The enzyme activity was slightly decreased in the presence of 1{\%} sodium dodecyl sulfate. The enzyme was somewhat susceptible to chymotrypsin treatment but resistant to digestion by trypsin.",
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Biochemical characterization of a novel 2-Cys peroxiredoxin from Antrodia camphorata. / Huang, Jenq Kuen; Ken, Chuian-Fu; Huang, Hui Ming; Lin, Chi Tsai.

In: Applied Microbiology and Biotechnology, Vol. 74, No. 1, 01.02.2007, p. 84-92.

Research output: Contribution to journalArticle

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