Biochemical characterization of a functional recombinant aryl-alcohol dehydrogenase from Taiwanofungus camphorata

Chuian-Fu Ken, Che Chi Chang, Lisa Wen, Jenq Kuen Huang, Chi Tsai Lin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Aryl-alcohol dehydrogenases (AADs) have been known to involve in the metabolism of aromatic compounds. Results: One TcAAD cDNA (GenBank HQ453361) encoding a putative aryl-alcohol dehydrogenase (AAD) was cloned from Taiwanofungus camphorata. The deduced amino acid sequence is conserved among the reported AADs. A 3-D structural model of the TcAAD has been created based on the known structure of voltage-dependent potassium channels subunit beta-2 (PDB code: 3EAU). To characterize the TcAAD, the coding region was subcloned into an expression vector and transformed into Saccharomyces cerevisiae. The recombinant His6-tagged TcAAD was overexpressed and purified by Ni affinity chromatography. The purified enzyme showed a band of approximately 39 kDa on a 12% SDS-PAGE. The molecular mass determined by MALDI-TOF is 40.58 kDa which suggests that the purified enzyme is a monomeric enzyme. Using veratraldehyde as a substrate, the KM, Vmax of TcADD was determined at pH 6.0. Using benzyl alcohol derivatives as substrates, the oxidizing power of TcADD via NAD+ at pH 9.6 was studied. Conclusions: The coding sequence of the TcAAD cDNA was introduced into an S. cerevisiae expression system and the active enzyme purified and characterized. Understanding the properties of this TcAAD will be beneficial for its potential in xenobiotic detoxification or production of natural flavors.

Original languageEnglish
JournalBotanical Studies
Volume55
Issue number1
DOIs
Publication statusPublished - 2014 Feb 2

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Taiwanofungus
alcohol dehydrogenase
enzymes
Saccharomyces cerevisiae
benzyl alcohol
potassium channels
xenobiotics
affinity chromatography
aromatic compounds
amino acid sequences
flavor
chemical derivatives
molecular weight
metabolism

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Ken, Chuian-Fu ; Chang, Che Chi ; Wen, Lisa ; Huang, Jenq Kuen ; Lin, Chi Tsai. / Biochemical characterization of a functional recombinant aryl-alcohol dehydrogenase from Taiwanofungus camphorata. In: Botanical Studies. 2014 ; Vol. 55, No. 1.
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abstract = "Background: Aryl-alcohol dehydrogenases (AADs) have been known to involve in the metabolism of aromatic compounds. Results: One TcAAD cDNA (GenBank HQ453361) encoding a putative aryl-alcohol dehydrogenase (AAD) was cloned from Taiwanofungus camphorata. The deduced amino acid sequence is conserved among the reported AADs. A 3-D structural model of the TcAAD has been created based on the known structure of voltage-dependent potassium channels subunit beta-2 (PDB code: 3EAU). To characterize the TcAAD, the coding region was subcloned into an expression vector and transformed into Saccharomyces cerevisiae. The recombinant His6-tagged TcAAD was overexpressed and purified by Ni affinity chromatography. The purified enzyme showed a band of approximately 39 kDa on a 12{\%} SDS-PAGE. The molecular mass determined by MALDI-TOF is 40.58 kDa which suggests that the purified enzyme is a monomeric enzyme. Using veratraldehyde as a substrate, the KM, Vmax of TcADD was determined at pH 6.0. Using benzyl alcohol derivatives as substrates, the oxidizing power of TcADD via NAD+ at pH 9.6 was studied. Conclusions: The coding sequence of the TcAAD cDNA was introduced into an S. cerevisiae expression system and the active enzyme purified and characterized. Understanding the properties of this TcAAD will be beneficial for its potential in xenobiotic detoxification or production of natural flavors.",
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Biochemical characterization of a functional recombinant aryl-alcohol dehydrogenase from Taiwanofungus camphorata. / Ken, Chuian-Fu; Chang, Che Chi; Wen, Lisa; Huang, Jenq Kuen; Lin, Chi Tsai.

In: Botanical Studies, Vol. 55, No. 1, 02.02.2014.

Research output: Contribution to journalArticle

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AU - Lin, Chi Tsai

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AB - Background: Aryl-alcohol dehydrogenases (AADs) have been known to involve in the metabolism of aromatic compounds. Results: One TcAAD cDNA (GenBank HQ453361) encoding a putative aryl-alcohol dehydrogenase (AAD) was cloned from Taiwanofungus camphorata. The deduced amino acid sequence is conserved among the reported AADs. A 3-D structural model of the TcAAD has been created based on the known structure of voltage-dependent potassium channels subunit beta-2 (PDB code: 3EAU). To characterize the TcAAD, the coding region was subcloned into an expression vector and transformed into Saccharomyces cerevisiae. The recombinant His6-tagged TcAAD was overexpressed and purified by Ni affinity chromatography. The purified enzyme showed a band of approximately 39 kDa on a 12% SDS-PAGE. The molecular mass determined by MALDI-TOF is 40.58 kDa which suggests that the purified enzyme is a monomeric enzyme. Using veratraldehyde as a substrate, the KM, Vmax of TcADD was determined at pH 6.0. Using benzyl alcohol derivatives as substrates, the oxidizing power of TcADD via NAD+ at pH 9.6 was studied. Conclusions: The coding sequence of the TcAAD cDNA was introduced into an S. cerevisiae expression system and the active enzyme purified and characterized. Understanding the properties of this TcAAD will be beneficial for its potential in xenobiotic detoxification or production of natural flavors.

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