Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering

Yu Fen Huang, Yang-Wei Lin, Zong Hong Lin, Huan Tsung Chang

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

In this study, we demonstrated the potential use of nucleic acid ligand (aptamers) conjugated gold nanoparticles (AuNPs) for cancer cell detection. Through specific binding of the aptamers toward platelet-derived growth factor (PDGF), MDA-MB-231 and Hs578T cells (cancer cells) that over-express PDGF, interact with Apt-AuNPs to a greater extent than do H184B5F5/M10 cells (normal cells). These results were confirmed through inductively coupled plasma mass spectrometry measurements of the gold ion concentrations within these cells. Aggregation of the Apt-AuNPs in the cytoplasm of the cancer cells led to the generation of an intense scattered light upon photo-illumination; this phenomenon allows the differentiation of cancer cells from normal cells using a dark field optical microscope. The presence of Apt-AuNPs suppressed the proliferation of MDA-MB-231 cancer cells, but not H184B5F5/M10 cells.

Original languageEnglish
Pages (from-to)775-783
Number of pages9
JournalJournal of Nanoparticle Research
Volume11
Issue number4
DOIs
Publication statusPublished - 2009 May 1

Fingerprint

Gold Nanoparticles
Light Scattering
Breast Cancer
breast
Gold
Light scattering
light scattering
cancer
Cells
gold
Nanoparticles
nanoparticles
Cell
cells
multiple docking adapters
Platelet-Derived Growth Factor
Platelets
Cancer
platelets
Inductively coupled plasma mass spectrometry

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Atomic and Molecular Physics, and Optics
  • Modelling and Simulation
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Huang, Yu Fen ; Lin, Yang-Wei ; Lin, Zong Hong ; Chang, Huan Tsung. / Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering. In: Journal of Nanoparticle Research. 2009 ; Vol. 11, No. 4. pp. 775-783.
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Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering. / Huang, Yu Fen; Lin, Yang-Wei; Lin, Zong Hong; Chang, Huan Tsung.

In: Journal of Nanoparticle Research, Vol. 11, No. 4, 01.05.2009, p. 775-783.

Research output: Contribution to journalArticle

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