Antioxidant capability of polysaccharides fractionated from submerge-cultured Agaricus blazei mycelia

Yaw Bee Ker, Kuan Chou Chen, Charng Cherng Chyau, Chin Chu Chen, Jia Hsin Guo, Chiu Lan Hsieh, Hui Er Wang, Chiung Chi Peng, Chi Huang Chang, Robert Y. Peng

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Five polysaccharide fractions (AB-1, AB-2, AB-3, AB-4, and AB-5) were obtained after a systemic solvent extraction and precipitation of Agaricus blazei mycelia with yields of 5.20, 9.03, 2.84, 17.47, and 0.44%, respectively. Among which, AB-1 and AB-3 demonstrated great DPPH radical scavenging ability around 85.0 and 72.0%, respectively, at a concentration of 5 mg/mL. The ferrous ion chelating powers were even more excellent at a concentration of 1 mg/mL, reaching almost over 99.65% for fractions AB-2, AB-3, and AB-4 as compared to the reference control of citric acid (35%); at a dosage of 5 mg/mL, fraction AB-1 even showed 105% in efficiency. In terms of the absolute chelating power (ACP), the mole numbers of ferrous (Fe2+) ions chelated were inversely related with the mean molecular mass of the polysaccharides in each fraction. In addition, gel permeation chromatography analysis showed that the more potent fractions were residing in those fractions with lower molecular masses, such as fractions AB-1 (757 kDa), AB-2 (887 kDa), and AB-4 (631 kDa) etc., and surprisingly, the free radical scavenging capability was also not closely correlated with the mean molecular masses, alternately being well-associated with the solubility of polysaccharides.

Original languageEnglish
Pages (from-to)7052-7058
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number18
DOIs
Publication statusPublished - 2005 Sep 7

Fingerprint

Agaricus subrufescens
Agaricus
Mycelium
Molecular mass
mycelium
Polysaccharides
polysaccharides
Antioxidants
Scavenging
molecular weight
Chelation
antioxidants
Ions
ions
Gel permeation chromatography
Solvent extraction
Citric Acid
citric acid
Solubility
Free Radicals

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ker, Yaw Bee ; Chen, Kuan Chou ; Chyau, Charng Cherng ; Chen, Chin Chu ; Guo, Jia Hsin ; Hsieh, Chiu Lan ; Wang, Hui Er ; Peng, Chiung Chi ; Chang, Chi Huang ; Peng, Robert Y. / Antioxidant capability of polysaccharides fractionated from submerge-cultured Agaricus blazei mycelia. In: Journal of Agricultural and Food Chemistry. 2005 ; Vol. 53, No. 18. pp. 7052-7058.
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title = "Antioxidant capability of polysaccharides fractionated from submerge-cultured Agaricus blazei mycelia",
abstract = "Five polysaccharide fractions (AB-1, AB-2, AB-3, AB-4, and AB-5) were obtained after a systemic solvent extraction and precipitation of Agaricus blazei mycelia with yields of 5.20, 9.03, 2.84, 17.47, and 0.44{\%}, respectively. Among which, AB-1 and AB-3 demonstrated great DPPH• radical scavenging ability around 85.0 and 72.0{\%}, respectively, at a concentration of 5 mg/mL. The ferrous ion chelating powers were even more excellent at a concentration of 1 mg/mL, reaching almost over 99.65{\%} for fractions AB-2, AB-3, and AB-4 as compared to the reference control of citric acid (35{\%}); at a dosage of 5 mg/mL, fraction AB-1 even showed 105{\%} in efficiency. In terms of the absolute chelating power (ACP), the mole numbers of ferrous (Fe2+) ions chelated were inversely related with the mean molecular mass of the polysaccharides in each fraction. In addition, gel permeation chromatography analysis showed that the more potent fractions were residing in those fractions with lower molecular masses, such as fractions AB-1 (757 kDa), AB-2 (887 kDa), and AB-4 (631 kDa) etc., and surprisingly, the free radical scavenging capability was also not closely correlated with the mean molecular masses, alternately being well-associated with the solubility of polysaccharides.",
author = "Ker, {Yaw Bee} and Chen, {Kuan Chou} and Chyau, {Charng Cherng} and Chen, {Chin Chu} and Guo, {Jia Hsin} and Hsieh, {Chiu Lan} and Wang, {Hui Er} and Peng, {Chiung Chi} and Chang, {Chi Huang} and Peng, {Robert Y.}",
year = "2005",
month = "9",
day = "7",
doi = "10.1021/jf0510034",
language = "English",
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Ker, YB, Chen, KC, Chyau, CC, Chen, CC, Guo, JH, Hsieh, CL, Wang, HE, Peng, CC, Chang, CH & Peng, RY 2005, 'Antioxidant capability of polysaccharides fractionated from submerge-cultured Agaricus blazei mycelia', Journal of Agricultural and Food Chemistry, vol. 53, no. 18, pp. 7052-7058. https://doi.org/10.1021/jf0510034

Antioxidant capability of polysaccharides fractionated from submerge-cultured Agaricus blazei mycelia. / Ker, Yaw Bee; Chen, Kuan Chou; Chyau, Charng Cherng; Chen, Chin Chu; Guo, Jia Hsin; Hsieh, Chiu Lan; Wang, Hui Er; Peng, Chiung Chi; Chang, Chi Huang; Peng, Robert Y.

In: Journal of Agricultural and Food Chemistry, Vol. 53, No. 18, 07.09.2005, p. 7052-7058.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant capability of polysaccharides fractionated from submerge-cultured Agaricus blazei mycelia

AU - Ker, Yaw Bee

AU - Chen, Kuan Chou

AU - Chyau, Charng Cherng

AU - Chen, Chin Chu

AU - Guo, Jia Hsin

AU - Hsieh, Chiu Lan

AU - Wang, Hui Er

AU - Peng, Chiung Chi

AU - Chang, Chi Huang

AU - Peng, Robert Y.

PY - 2005/9/7

Y1 - 2005/9/7

N2 - Five polysaccharide fractions (AB-1, AB-2, AB-3, AB-4, and AB-5) were obtained after a systemic solvent extraction and precipitation of Agaricus blazei mycelia with yields of 5.20, 9.03, 2.84, 17.47, and 0.44%, respectively. Among which, AB-1 and AB-3 demonstrated great DPPH• radical scavenging ability around 85.0 and 72.0%, respectively, at a concentration of 5 mg/mL. The ferrous ion chelating powers were even more excellent at a concentration of 1 mg/mL, reaching almost over 99.65% for fractions AB-2, AB-3, and AB-4 as compared to the reference control of citric acid (35%); at a dosage of 5 mg/mL, fraction AB-1 even showed 105% in efficiency. In terms of the absolute chelating power (ACP), the mole numbers of ferrous (Fe2+) ions chelated were inversely related with the mean molecular mass of the polysaccharides in each fraction. In addition, gel permeation chromatography analysis showed that the more potent fractions were residing in those fractions with lower molecular masses, such as fractions AB-1 (757 kDa), AB-2 (887 kDa), and AB-4 (631 kDa) etc., and surprisingly, the free radical scavenging capability was also not closely correlated with the mean molecular masses, alternately being well-associated with the solubility of polysaccharides.

AB - Five polysaccharide fractions (AB-1, AB-2, AB-3, AB-4, and AB-5) were obtained after a systemic solvent extraction and precipitation of Agaricus blazei mycelia with yields of 5.20, 9.03, 2.84, 17.47, and 0.44%, respectively. Among which, AB-1 and AB-3 demonstrated great DPPH• radical scavenging ability around 85.0 and 72.0%, respectively, at a concentration of 5 mg/mL. The ferrous ion chelating powers were even more excellent at a concentration of 1 mg/mL, reaching almost over 99.65% for fractions AB-2, AB-3, and AB-4 as compared to the reference control of citric acid (35%); at a dosage of 5 mg/mL, fraction AB-1 even showed 105% in efficiency. In terms of the absolute chelating power (ACP), the mole numbers of ferrous (Fe2+) ions chelated were inversely related with the mean molecular mass of the polysaccharides in each fraction. In addition, gel permeation chromatography analysis showed that the more potent fractions were residing in those fractions with lower molecular masses, such as fractions AB-1 (757 kDa), AB-2 (887 kDa), and AB-4 (631 kDa) etc., and surprisingly, the free radical scavenging capability was also not closely correlated with the mean molecular masses, alternately being well-associated with the solubility of polysaccharides.

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